Degradation Parameters from Pulse-Chase Experiments.
Pulse-chase experiments are often used to study the degradation of macromolecules such as proteins or mRNA. Considerations for the choice of pulse length include the toxicity of the pulse to the cell and maximization of labeling. In the general case of non-exponential decay, varying the length of th...
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| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0155028&type=printable |
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| _version_ | 1849723418943946752 |
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| author | Celine Sin Davide Chiarugi Angelo Valleriani |
| author_facet | Celine Sin Davide Chiarugi Angelo Valleriani |
| author_sort | Celine Sin |
| collection | DOAJ |
| description | Pulse-chase experiments are often used to study the degradation of macromolecules such as proteins or mRNA. Considerations for the choice of pulse length include the toxicity of the pulse to the cell and maximization of labeling. In the general case of non-exponential decay, varying the length of the pulse results in decay patterns that look different. Analysis of these patterns without consideration to pulse length would yield incorrect degradation parameters. Here we propose a method that constructively includes pulse length in the analysis of decay patterns and extracts the parameters of the underlying degradation process. We also show how to extract decay parameters reliably from measurements taken during the pulse phase. |
| format | Article |
| id | doaj-art-b922dca8bfe740d5b8f8acb6fba7f54c |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-b922dca8bfe740d5b8f8acb6fba7f54c2025-08-20T03:11:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01115e015502810.1371/journal.pone.0155028Degradation Parameters from Pulse-Chase Experiments.Celine SinDavide ChiarugiAngelo VallerianiPulse-chase experiments are often used to study the degradation of macromolecules such as proteins or mRNA. Considerations for the choice of pulse length include the toxicity of the pulse to the cell and maximization of labeling. In the general case of non-exponential decay, varying the length of the pulse results in decay patterns that look different. Analysis of these patterns without consideration to pulse length would yield incorrect degradation parameters. Here we propose a method that constructively includes pulse length in the analysis of decay patterns and extracts the parameters of the underlying degradation process. We also show how to extract decay parameters reliably from measurements taken during the pulse phase.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0155028&type=printable |
| spellingShingle | Celine Sin Davide Chiarugi Angelo Valleriani Degradation Parameters from Pulse-Chase Experiments. PLoS ONE |
| title | Degradation Parameters from Pulse-Chase Experiments. |
| title_full | Degradation Parameters from Pulse-Chase Experiments. |
| title_fullStr | Degradation Parameters from Pulse-Chase Experiments. |
| title_full_unstemmed | Degradation Parameters from Pulse-Chase Experiments. |
| title_short | Degradation Parameters from Pulse-Chase Experiments. |
| title_sort | degradation parameters from pulse chase experiments |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0155028&type=printable |
| work_keys_str_mv | AT celinesin degradationparametersfrompulsechaseexperiments AT davidechiarugi degradationparametersfrompulsechaseexperiments AT angelovalleriani degradationparametersfrompulsechaseexperiments |