Effect of EGCG–Methacrylate-Functionalized Resin Infiltrant on White Spot Lesions: An In Vitro Study

Effect of EGCG–Methacrylate-Functionalized Resin Infiltrant on White Spot Lesions: An In Vitro Study

This study evaluated the color change (ΔE<sub>00</sub>) and penetration depth (PD) of white spot lesions (WSLs) infiltrated with the resin infiltrant (Icon<sup>®</sup>) functionalized with methacrylate epigallocatechin-3-gallate (EGCG). To introduce polymerizable double bonds...

Full description

Saved in:
Bibliographic Details
Main Authors: Karin Landmayer, Bruna de Oliveira Iatarola, Talita Portela Pereira, Raquel Shimizu Mori, Alyssa Teixeira Obeid, Mariele Vertuan, Daniela Alvim Chrisostomo, Ana Carolina Magalhães, Lulwah Alreshaid, Paulo Henrique dos Santos, Anuradha Prakki, Luciana Fávaro Francisconi-dos-Rios
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Journal of Functional Biomaterials
Subjects:
antimicrobial agents
confocal laser scanning microscopy
dental white spots
catechin
icon infiltrant
ultraviolet spectrophotometry
Online Access:https://www.mdpi.com/2079-4983/16/1/6
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study evaluated the color change (ΔE<sub>00</sub>) and penetration depth (PD) of white spot lesions (WSLs) infiltrated with the resin infiltrant (Icon<sup>®</sup>) functionalized with methacrylate epigallocatechin-3-gallate (EGCG). To introduce polymerizable double bonds, EGCG was reacted with methacryloyl chloride (EM). Subsequently, the Icon resin infiltrant (I) was loaded with neat EGCG (IE) or EGCG–methacrylate (IEM) at 2 wt% each. WSLs were created on bovine enamel blocks and treated with I, IE, or IEM. Sound and untreated enamel surfaces were used as controls (C). Infiltrant PD (%) was determined by Confocal Laser Scanning Microscopy (CLSM, n = 12) analysis. For color change (ΔE<sub>00</sub>) determination (n = 14), ΔL, Δa, and Δb, half of each sample was kept sound as a reference area. The color was determined with a spectrophotometer. Data were statistically evaluated (<i>p</i> = 0.05). Surface morphology was obtained as a qualitative response variable using 3D CLSM. PD (%) did not differ statistically for I, IE, and IEM (<i>p</i> = 0.780). Groups I and IEM showed similar performance on color change (ΔE<sub>00</sub>) compared to the control group, while IE exhibited intermediate results, with no significant difference observed between the untreated, I, and IEM groups (<i>p</i> < 0.001). IEM promoted the masking of the WSL color without interfering with the PD.
ISSN:2079-4983

Similar Items