Structural characteristics, anticoagulant and antithrombotic mechanism of a novel polysaccharide from Rosa Chinensis Flos
This is the first report on a polysaccharide (RCJ2-Ib) isolated from Rosa Chinensis Flos. RCJ2-Ib was obtained through the extraction with water, precipitation with ethanol, separation with DEAE-52 column and purification with DEAE-Sepharose Fast Flow column and Sephadex G100 column. GC, FT-IR and N...
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| Language: | English |
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Tsinghua University Press
2023-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001549 |
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| author | Xiaofeng Zhang Zhenhua Liang Geoffrey Ivan Neil Waterhouse Shengjun Jiang Dongxiao Sun-Waterhouse Jinmei Wang Changyang Ma Wenyi Kang |
| author_facet | Xiaofeng Zhang Zhenhua Liang Geoffrey Ivan Neil Waterhouse Shengjun Jiang Dongxiao Sun-Waterhouse Jinmei Wang Changyang Ma Wenyi Kang |
| author_sort | Xiaofeng Zhang |
| collection | DOAJ |
| description | This is the first report on a polysaccharide (RCJ2-Ib) isolated from Rosa Chinensis Flos. RCJ2-Ib was obtained through the extraction with water, precipitation with ethanol, separation with DEAE-52 column and purification with DEAE-Sepharose Fast Flow column and Sephadex G100 column. GC, FT-IR and NMR analyses revealed that RCJ2-Ib (3.3 kDa) was a 1,4-linked polymannuronic acid containing substantial β-D-anomers units. The anticoagulant effect of RCJ2-Ib evaluated by using rabbit ear venous blood and an acute blood stasis rat model showed that RCJ2-Ib had obvious anticoagulant activity in regulating endogenous and exogenous coagulation pathways and reducing serum thromboxane B2 and endothelin-1. In addition, RCJ2-Ib could also increase the number of Lactobacillus and Escherichia coli. As a result, RCJ2-Ib has the potential to inhibit thrombosis and maintain the intestinal environment. |
| format | Article |
| id | doaj-art-b8c231739b794a5dbb0b46f2120ef26c |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-b8c231739b794a5dbb0b46f2120ef26c2025-02-03T06:55:49ZengTsinghua University PressFood Science and Human Wellness2213-45302023-03-01122407415Structural characteristics, anticoagulant and antithrombotic mechanism of a novel polysaccharide from Rosa Chinensis FlosXiaofeng Zhang0Zhenhua Liang1Geoffrey Ivan Neil Waterhouse2Shengjun Jiang3Dongxiao Sun-Waterhouse4Jinmei Wang5Changyang Ma6Wenyi Kang7National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Kaifeng 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Kaifeng 475004, ChinaSchool of Chemical Sciences, University of Auckland, Auckland 1142, New ZealandNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, ChinaSchool of Chemical Sciences, University of Auckland, Auckland 1142, New ZealandNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Kaifeng 475004, China; Corresponding authors.National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, China; Corresponding authors.National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Kaifeng 475004, China; Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, China; Corresponding authors.This is the first report on a polysaccharide (RCJ2-Ib) isolated from Rosa Chinensis Flos. RCJ2-Ib was obtained through the extraction with water, precipitation with ethanol, separation with DEAE-52 column and purification with DEAE-Sepharose Fast Flow column and Sephadex G100 column. GC, FT-IR and NMR analyses revealed that RCJ2-Ib (3.3 kDa) was a 1,4-linked polymannuronic acid containing substantial β-D-anomers units. The anticoagulant effect of RCJ2-Ib evaluated by using rabbit ear venous blood and an acute blood stasis rat model showed that RCJ2-Ib had obvious anticoagulant activity in regulating endogenous and exogenous coagulation pathways and reducing serum thromboxane B2 and endothelin-1. In addition, RCJ2-Ib could also increase the number of Lactobacillus and Escherichia coli. As a result, RCJ2-Ib has the potential to inhibit thrombosis and maintain the intestinal environment.http://www.sciencedirect.com/science/article/pii/S2213453022001549Rosa Chinensis Flospolysaccharide isolationantithrombotic activityintestinal flora |
| spellingShingle | Xiaofeng Zhang Zhenhua Liang Geoffrey Ivan Neil Waterhouse Shengjun Jiang Dongxiao Sun-Waterhouse Jinmei Wang Changyang Ma Wenyi Kang Structural characteristics, anticoagulant and antithrombotic mechanism of a novel polysaccharide from Rosa Chinensis Flos Food Science and Human Wellness Rosa Chinensis Flos polysaccharide isolation antithrombotic activity intestinal flora |
| title | Structural characteristics, anticoagulant and antithrombotic mechanism of a novel polysaccharide from Rosa Chinensis Flos |
| title_full | Structural characteristics, anticoagulant and antithrombotic mechanism of a novel polysaccharide from Rosa Chinensis Flos |
| title_fullStr | Structural characteristics, anticoagulant and antithrombotic mechanism of a novel polysaccharide from Rosa Chinensis Flos |
| title_full_unstemmed | Structural characteristics, anticoagulant and antithrombotic mechanism of a novel polysaccharide from Rosa Chinensis Flos |
| title_short | Structural characteristics, anticoagulant and antithrombotic mechanism of a novel polysaccharide from Rosa Chinensis Flos |
| title_sort | structural characteristics anticoagulant and antithrombotic mechanism of a novel polysaccharide from rosa chinensis flos |
| topic | Rosa Chinensis Flos polysaccharide isolation antithrombotic activity intestinal flora |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001549 |
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