RNA splicing: a split consensus reveals two major 5′ splice site classes
The established consensus sequence for human 5′ splice sites masks the presence of two major splice site classes defined by preferential base-pairing potentials with either U5 snRNA loop 1 or the U6 snRNA ACAGA box. The two 5′ splice site classes are separable in genome sequences, sensitized by spec...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Royal Society
2025-01-01
|
| Series: | Open Biology |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsob.240293 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832593698493300736 |
|---|---|
| author | Matthew T. Parker Sebastian M. Fica Gordon G. Simpson |
| author_facet | Matthew T. Parker Sebastian M. Fica Gordon G. Simpson |
| author_sort | Matthew T. Parker |
| collection | DOAJ |
| description | The established consensus sequence for human 5′ splice sites masks the presence of two major splice site classes defined by preferential base-pairing potentials with either U5 snRNA loop 1 or the U6 snRNA ACAGA box. The two 5′ splice site classes are separable in genome sequences, sensitized by specific genotypes and associated with splicing complexity. The two classes reflect the commitment to 5′ splice site usage occurring primarily during 5′ splice site transfer to U6 snRNA. Separating the human 5′ splice site consensus into its two major constituents can help us understand fundamental features of eukaryote genome architecture and splicing mechanisms and inform treatment design for diseases caused by genetic variation affecting splicing. |
| format | Article |
| id | doaj-art-b8bbbe92b84149449392fdb0868b6e5c |
| institution | Kabale University |
| issn | 2046-2441 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj-art-b8bbbe92b84149449392fdb0868b6e5c2025-01-20T10:18:56ZengThe Royal SocietyOpen Biology2046-24412025-01-0115110.1098/rsob.240293RNA splicing: a split consensus reveals two major 5′ splice site classesMatthew T. Parker0Sebastian M. Fica1Gordon G. Simpson2Max Planck Institute for Plant Breeding Research, Cologne, GermanyDepartment of Biochemistry, University of Oxford, Oxford, UKSchool of Life Sciences, University of Dundee, Dundee, UKThe established consensus sequence for human 5′ splice sites masks the presence of two major splice site classes defined by preferential base-pairing potentials with either U5 snRNA loop 1 or the U6 snRNA ACAGA box. The two 5′ splice site classes are separable in genome sequences, sensitized by specific genotypes and associated with splicing complexity. The two classes reflect the commitment to 5′ splice site usage occurring primarily during 5′ splice site transfer to U6 snRNA. Separating the human 5′ splice site consensus into its two major constituents can help us understand fundamental features of eukaryote genome architecture and splicing mechanisms and inform treatment design for diseases caused by genetic variation affecting splicing.https://royalsocietypublishing.org/doi/10.1098/rsob.240293splicingMETTL16SNRNP27Km6AT-loopReNU syndrome |
| spellingShingle | Matthew T. Parker Sebastian M. Fica Gordon G. Simpson RNA splicing: a split consensus reveals two major 5′ splice site classes Open Biology splicing METTL16 SNRNP27K m6A T-loop ReNU syndrome |
| title | RNA splicing: a split consensus reveals two major 5′ splice site classes |
| title_full | RNA splicing: a split consensus reveals two major 5′ splice site classes |
| title_fullStr | RNA splicing: a split consensus reveals two major 5′ splice site classes |
| title_full_unstemmed | RNA splicing: a split consensus reveals two major 5′ splice site classes |
| title_short | RNA splicing: a split consensus reveals two major 5′ splice site classes |
| title_sort | rna splicing a split consensus reveals two major 5 splice site classes |
| topic | splicing METTL16 SNRNP27K m6A T-loop ReNU syndrome |
| url | https://royalsocietypublishing.org/doi/10.1098/rsob.240293 |
| work_keys_str_mv | AT matthewtparker rnasplicingasplitconsensusrevealstwomajor5splicesiteclasses AT sebastianmfica rnasplicingasplitconsensusrevealstwomajor5splicesiteclasses AT gordongsimpson rnasplicingasplitconsensusrevealstwomajor5splicesiteclasses |