Proteomics and phosphoproteomics of freshwater mollusk carcasses reveal novel insights as potential food source
Cipangopaludina chinensis, Radix auricularia, and Nodularia douglasiae are common freshwater mollusks widely distributed in lakes and rivers across China. In this study, (DIA) Data - independent acquisition method was used to analyze the proteomics and phosphoproteomics among three species. The resu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1568349/full |
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| author | Gongzhen Liu Shengkang Wang Tielin Wang Changan Zhu Changlong Li Xinyi Zhang |
| author_facet | Gongzhen Liu Shengkang Wang Tielin Wang Changan Zhu Changlong Li Xinyi Zhang |
| author_sort | Gongzhen Liu |
| collection | DOAJ |
| description | Cipangopaludina chinensis, Radix auricularia, and Nodularia douglasiae are common freshwater mollusks widely distributed in lakes and rivers across China. In this study, (DIA) Data - independent acquisition method was used to analyze the proteomics and phosphoproteomics among three species. The results showed that a total of 1,382 proteins were identified from mollusk carcasses, with 492 proteins were quantitively analyzed. These proteins are mainly involved in amino acid nutrition and metabolism, including glutamate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, and tyrosinase copper-binding domain-containing protein. Bioinformatics analysis revealed that the identified proteins are primarily involved in carbon metabolism, microbial metabolism, ubiquitin-mediated proteolysis, and RAS signaling pathways. Notably, this study offers valuable insights into the differential protein expression of proteins across species. Furthermore, proteomics analysis revealed several novel proteins, which helps to improve our understanding of amino acid metabolism, protein diversity, and signaling pathways in these freshwater mollusks. |
| format | Article |
| id | doaj-art-b8b9b4d6dd6e4d59a079f16366d9baf0 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-b8b9b4d6dd6e4d59a079f16366d9baf02025-08-20T04:00:56ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-08-011510.3389/fcimb.2025.15683491568349Proteomics and phosphoproteomics of freshwater mollusk carcasses reveal novel insights as potential food sourceGongzhen LiuShengkang WangTielin WangChangan ZhuChanglong LiXinyi ZhangCipangopaludina chinensis, Radix auricularia, and Nodularia douglasiae are common freshwater mollusks widely distributed in lakes and rivers across China. In this study, (DIA) Data - independent acquisition method was used to analyze the proteomics and phosphoproteomics among three species. The results showed that a total of 1,382 proteins were identified from mollusk carcasses, with 492 proteins were quantitively analyzed. These proteins are mainly involved in amino acid nutrition and metabolism, including glutamate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, and tyrosinase copper-binding domain-containing protein. Bioinformatics analysis revealed that the identified proteins are primarily involved in carbon metabolism, microbial metabolism, ubiquitin-mediated proteolysis, and RAS signaling pathways. Notably, this study offers valuable insights into the differential protein expression of proteins across species. Furthermore, proteomics analysis revealed several novel proteins, which helps to improve our understanding of amino acid metabolism, protein diversity, and signaling pathways in these freshwater mollusks.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1568349/fullfreshwatermollusks carcassesproteomicsphosphoproteomicscellular componentsmolecular functions |
| spellingShingle | Gongzhen Liu Shengkang Wang Tielin Wang Changan Zhu Changlong Li Xinyi Zhang Proteomics and phosphoproteomics of freshwater mollusk carcasses reveal novel insights as potential food source Frontiers in Cellular and Infection Microbiology freshwater mollusks carcasses proteomics phosphoproteomics cellular components molecular functions |
| title | Proteomics and phosphoproteomics of freshwater mollusk carcasses reveal novel insights as potential food source |
| title_full | Proteomics and phosphoproteomics of freshwater mollusk carcasses reveal novel insights as potential food source |
| title_fullStr | Proteomics and phosphoproteomics of freshwater mollusk carcasses reveal novel insights as potential food source |
| title_full_unstemmed | Proteomics and phosphoproteomics of freshwater mollusk carcasses reveal novel insights as potential food source |
| title_short | Proteomics and phosphoproteomics of freshwater mollusk carcasses reveal novel insights as potential food source |
| title_sort | proteomics and phosphoproteomics of freshwater mollusk carcasses reveal novel insights as potential food source |
| topic | freshwater mollusks carcasses proteomics phosphoproteomics cellular components molecular functions |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1568349/full |
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