Antimicrobial and Antibiofilm Activities of 4,5-Dihydro-1H-pyrazole-1-carboximidamide Hydrochloride against Salmonella spp.
In the present study, the antimicrobial and antibiofilm activities of two 4,5-dihydro-1H-pyrazole-1-carboximidamide hydrochloride, (trifluoromethyl) phenyl-substituted (compound 1) and bromophenyl-substituted (compound 2), were evaluated against four Salmonella spp. serotypes through broth microdilu...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2021/5587318 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832557049357008896 |
|---|---|
| author | Andressa C. Damim Danilo Y. de Albuquerque Fabiana G. S. Dantas Fernanda Galvão Melyssa Negri Lucas Pizzuti Gleison A. Casagrande Sumbal Saba Jamal Rafique Kelly M. P. de Oliveira |
| author_facet | Andressa C. Damim Danilo Y. de Albuquerque Fabiana G. S. Dantas Fernanda Galvão Melyssa Negri Lucas Pizzuti Gleison A. Casagrande Sumbal Saba Jamal Rafique Kelly M. P. de Oliveira |
| author_sort | Andressa C. Damim |
| collection | DOAJ |
| description | In the present study, the antimicrobial and antibiofilm activities of two 4,5-dihydro-1H-pyrazole-1-carboximidamide hydrochloride, (trifluoromethyl) phenyl-substituted (compound 1) and bromophenyl-substituted (compound 2), were evaluated against four Salmonella spp. serotypes through broth microdilution and biofilm-forming activity. Further, the cytotoxicity of the compounds was evaluated by cell viability assays using cultures of HeLa and Vero cell lines, and the mutagenic potential was assessed by the Ames test. In the broth microdilution test, compound 1 inhibited 90% of the strains tested at the minimum inhibitory concentration of 62.5 μg mL−1. Furthermore, both compounds prevented biofilm formation, with a reduction of up to 5.2 log10. HeLa and Vero cells exhibited 100% viability in the presence of compound 1. In contrast, low cell viability was observed in the presence of 15 µg mL−1 of compound 2. Furthermore, no mutagenic potential was detected at any of the tested concentrations of compound 1. |
| format | Article |
| id | doaj-art-b8b314417edc481788471f223ad82b8d |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-b8b314417edc481788471f223ad82b8d2025-02-03T05:43:46ZengWileyJournal of Chemistry2090-90632090-90712021-01-01202110.1155/2021/55873185587318Antimicrobial and Antibiofilm Activities of 4,5-Dihydro-1H-pyrazole-1-carboximidamide Hydrochloride against Salmonella spp.Andressa C. Damim0Danilo Y. de Albuquerque1Fabiana G. S. Dantas2Fernanda Galvão3Melyssa Negri4Lucas Pizzuti5Gleison A. Casagrande6Sumbal Saba7Jamal Rafique8Kelly M. P. de Oliveira9Faculty of Exact Sciences and Technology, Federal University of Grande Dourados (UFGD, Dourados 79804970, MS, BrazilFaculty of Exact Sciences and Technology, Federal University of Grande Dourados (UFGD, Dourados 79804970, MS, BrazilFaculty of Biological and Environmental Sciences, Federal University of Grande Dourados (UFGD), Dourados 79804970, MS, BrazilFaculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados 79804970, MS, BrazilDepartment of Clinical Analysis and Biomedicine, State University of Maringá (UEM), Maringá 87020-900, PR, BrazilFaculty of Exact Sciences and Technology, Federal University of Grande Dourados (UFGD, Dourados 79804970, MS, BrazilInstitute of Chemistry (INQUI), Federal University of Mato Grosso Do Sul (UFMS), Campo Grande 79074-460, MS, BrazilInstitute of Chemistry (IQ), Federal University of Goiás (UFG), Goiânia 74690-900, GO, BrazilInstitute of Chemistry (INQUI), Federal University of Mato Grosso Do Sul (UFMS), Campo Grande 79074-460, MS, BrazilFaculty of Biological and Environmental Sciences, Federal University of Grande Dourados (UFGD), Dourados 79804970, MS, BrazilIn the present study, the antimicrobial and antibiofilm activities of two 4,5-dihydro-1H-pyrazole-1-carboximidamide hydrochloride, (trifluoromethyl) phenyl-substituted (compound 1) and bromophenyl-substituted (compound 2), were evaluated against four Salmonella spp. serotypes through broth microdilution and biofilm-forming activity. Further, the cytotoxicity of the compounds was evaluated by cell viability assays using cultures of HeLa and Vero cell lines, and the mutagenic potential was assessed by the Ames test. In the broth microdilution test, compound 1 inhibited 90% of the strains tested at the minimum inhibitory concentration of 62.5 μg mL−1. Furthermore, both compounds prevented biofilm formation, with a reduction of up to 5.2 log10. HeLa and Vero cells exhibited 100% viability in the presence of compound 1. In contrast, low cell viability was observed in the presence of 15 µg mL−1 of compound 2. Furthermore, no mutagenic potential was detected at any of the tested concentrations of compound 1.http://dx.doi.org/10.1155/2021/5587318 |
| spellingShingle | Andressa C. Damim Danilo Y. de Albuquerque Fabiana G. S. Dantas Fernanda Galvão Melyssa Negri Lucas Pizzuti Gleison A. Casagrande Sumbal Saba Jamal Rafique Kelly M. P. de Oliveira Antimicrobial and Antibiofilm Activities of 4,5-Dihydro-1H-pyrazole-1-carboximidamide Hydrochloride against Salmonella spp. Journal of Chemistry |
| title | Antimicrobial and Antibiofilm Activities of 4,5-Dihydro-1H-pyrazole-1-carboximidamide Hydrochloride against Salmonella spp. |
| title_full | Antimicrobial and Antibiofilm Activities of 4,5-Dihydro-1H-pyrazole-1-carboximidamide Hydrochloride against Salmonella spp. |
| title_fullStr | Antimicrobial and Antibiofilm Activities of 4,5-Dihydro-1H-pyrazole-1-carboximidamide Hydrochloride against Salmonella spp. |
| title_full_unstemmed | Antimicrobial and Antibiofilm Activities of 4,5-Dihydro-1H-pyrazole-1-carboximidamide Hydrochloride against Salmonella spp. |
| title_short | Antimicrobial and Antibiofilm Activities of 4,5-Dihydro-1H-pyrazole-1-carboximidamide Hydrochloride against Salmonella spp. |
| title_sort | antimicrobial and antibiofilm activities of 4 5 dihydro 1h pyrazole 1 carboximidamide hydrochloride against salmonella spp |
| url | http://dx.doi.org/10.1155/2021/5587318 |
| work_keys_str_mv | AT andressacdamim antimicrobialandantibiofilmactivitiesof45dihydro1hpyrazole1carboximidamidehydrochlorideagainstsalmonellaspp AT daniloydealbuquerque antimicrobialandantibiofilmactivitiesof45dihydro1hpyrazole1carboximidamidehydrochlorideagainstsalmonellaspp AT fabianagsdantas antimicrobialandantibiofilmactivitiesof45dihydro1hpyrazole1carboximidamidehydrochlorideagainstsalmonellaspp AT fernandagalvao antimicrobialandantibiofilmactivitiesof45dihydro1hpyrazole1carboximidamidehydrochlorideagainstsalmonellaspp AT melyssanegri antimicrobialandantibiofilmactivitiesof45dihydro1hpyrazole1carboximidamidehydrochlorideagainstsalmonellaspp AT lucaspizzuti antimicrobialandantibiofilmactivitiesof45dihydro1hpyrazole1carboximidamidehydrochlorideagainstsalmonellaspp AT gleisonacasagrande antimicrobialandantibiofilmactivitiesof45dihydro1hpyrazole1carboximidamidehydrochlorideagainstsalmonellaspp AT sumbalsaba antimicrobialandantibiofilmactivitiesof45dihydro1hpyrazole1carboximidamidehydrochlorideagainstsalmonellaspp AT jamalrafique antimicrobialandantibiofilmactivitiesof45dihydro1hpyrazole1carboximidamidehydrochlorideagainstsalmonellaspp AT kellympdeoliveira antimicrobialandantibiofilmactivitiesof45dihydro1hpyrazole1carboximidamidehydrochlorideagainstsalmonellaspp |