Expression of VEGF-A, Otx Homeobox and p53 Family Genes in Proliferative Vitreoretinopathy
Introduction. Proliferative vitreoretinopathy (PVR) is a severe inflammatory complication of retinal detachment. Pathological epiretinal membranes grow on the retina surface leading to contraction, and surgery fails in 5% to 10% of the cases. We evaluated the expression of VEGF-A, Otx1, Otx2, Otx3,...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/857380 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832563727200681984 |
|---|---|
| author | Claudio Azzolini Ilaria Stefania Pagani Cristina Pirrone Davide Borroni Simone Donati Muna Al Oum Diana Pigni Anna Maria Chiaravalli Riccardo Vinciguerra Francesca Simonelli Giovanni Porta |
| author_facet | Claudio Azzolini Ilaria Stefania Pagani Cristina Pirrone Davide Borroni Simone Donati Muna Al Oum Diana Pigni Anna Maria Chiaravalli Riccardo Vinciguerra Francesca Simonelli Giovanni Porta |
| author_sort | Claudio Azzolini |
| collection | DOAJ |
| description | Introduction. Proliferative vitreoretinopathy (PVR) is a severe inflammatory complication of retinal detachment. Pathological epiretinal membranes grow on the retina surface leading to contraction, and surgery fails in 5% to 10% of the cases. We evaluated the expression of VEGF-A, Otx1, Otx2, Otx3, and p53 family members from PVR specimens to correlate their role in inducing or preventing the pathology. Methods. Twelve retinal samples were taken from patients affected by PVR during therapeutic retinectomies in vitreoretinal surgery. Gene expression was evaluated using quantitative real-time reverse transcriptase PCR analysis and immunohistochemistry, using four healthy human retinae as control. Result. Controls showed basal expression of all genes. PVR samples showed little or no expression of Otx1 and variable expression of VEGF-A, Otx2, Otx3, p53, and p63 genes. Significant correlation was found among VEGF-A, Otx2, p53, and p63 and between Otx1 and Otx3. Conclusions. Otx homeobox, p53 family, and VEGF-A genes are expressed in PVR human retina. We individuated two possible pathways (VEGF-A, Otx2, p53, p63 and Otx1 and Otx3) involved in PVR progression that could influence in different manners the course of the pathology. Individuating the genetic pathways of PVR represents a novel approach to PVR therapies. |
| format | Article |
| id | doaj-art-b8acc0711f864d0280a614aaeb696322 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-b8acc0711f864d0280a614aaeb6963222025-02-03T01:12:52ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/857380857380Expression of VEGF-A, Otx Homeobox and p53 Family Genes in Proliferative VitreoretinopathyClaudio Azzolini0Ilaria Stefania Pagani1Cristina Pirrone2Davide Borroni3Simone Donati4Muna Al Oum5Diana Pigni6Anna Maria Chiaravalli7Riccardo Vinciguerra8Francesca Simonelli9Giovanni Porta10Department of Surgical and Morphological Sciences, Section of Ophthalmology, University of Insubria, Ospedale di Circolo, Via F. Guicciardini 9, 21100 Varese, ItalyDepartment of Clinical and Experimental Medicine, University of Insubria, Via O. Rossi 9, 21100 Varese, ItalyDepartment of Clinical and Experimental Medicine, University of Insubria, Via O. Rossi 9, 21100 Varese, ItalyDepartment of Surgical and Morphological Sciences, Section of Ophthalmology, University of Insubria, Ospedale di Circolo, Via F. Guicciardini 9, 21100 Varese, ItalyDepartment of Surgical and Morphological Sciences, Section of Ophthalmology, University of Insubria, Ospedale di Circolo, Via F. Guicciardini 9, 21100 Varese, ItalyDepartment of Surgical and Morphological Sciences, Section of Ophthalmology, University of Insubria, Ospedale di Circolo, Via F. Guicciardini 9, 21100 Varese, ItalyDepartment of Clinical and Experimental Medicine, University of Insubria, Via O. Rossi 9, 21100 Varese, ItalyPathology Institute, Ospedale di Circolo, Via F. Guicciardini 9, 21100 Varese, ItalyDepartment of Surgical and Morphological Sciences, Section of Ophthalmology, University of Insubria, Ospedale di Circolo, Via F. Guicciardini 9, 21100 Varese, ItalyEye Clinic, Second University of Napoli, Via S. Pansini 5, 80131 Napoli, ItalyDepartment of Clinical and Experimental Medicine, University of Insubria, Via O. Rossi 9, 21100 Varese, ItalyIntroduction. Proliferative vitreoretinopathy (PVR) is a severe inflammatory complication of retinal detachment. Pathological epiretinal membranes grow on the retina surface leading to contraction, and surgery fails in 5% to 10% of the cases. We evaluated the expression of VEGF-A, Otx1, Otx2, Otx3, and p53 family members from PVR specimens to correlate their role in inducing or preventing the pathology. Methods. Twelve retinal samples were taken from patients affected by PVR during therapeutic retinectomies in vitreoretinal surgery. Gene expression was evaluated using quantitative real-time reverse transcriptase PCR analysis and immunohistochemistry, using four healthy human retinae as control. Result. Controls showed basal expression of all genes. PVR samples showed little or no expression of Otx1 and variable expression of VEGF-A, Otx2, Otx3, p53, and p63 genes. Significant correlation was found among VEGF-A, Otx2, p53, and p63 and between Otx1 and Otx3. Conclusions. Otx homeobox, p53 family, and VEGF-A genes are expressed in PVR human retina. We individuated two possible pathways (VEGF-A, Otx2, p53, p63 and Otx1 and Otx3) involved in PVR progression that could influence in different manners the course of the pathology. Individuating the genetic pathways of PVR represents a novel approach to PVR therapies.http://dx.doi.org/10.1155/2013/857380 |
| spellingShingle | Claudio Azzolini Ilaria Stefania Pagani Cristina Pirrone Davide Borroni Simone Donati Muna Al Oum Diana Pigni Anna Maria Chiaravalli Riccardo Vinciguerra Francesca Simonelli Giovanni Porta Expression of VEGF-A, Otx Homeobox and p53 Family Genes in Proliferative Vitreoretinopathy Mediators of Inflammation |
| title | Expression of VEGF-A, Otx Homeobox and p53 Family Genes in Proliferative Vitreoretinopathy |
| title_full | Expression of VEGF-A, Otx Homeobox and p53 Family Genes in Proliferative Vitreoretinopathy |
| title_fullStr | Expression of VEGF-A, Otx Homeobox and p53 Family Genes in Proliferative Vitreoretinopathy |
| title_full_unstemmed | Expression of VEGF-A, Otx Homeobox and p53 Family Genes in Proliferative Vitreoretinopathy |
| title_short | Expression of VEGF-A, Otx Homeobox and p53 Family Genes in Proliferative Vitreoretinopathy |
| title_sort | expression of vegf a otx homeobox and p53 family genes in proliferative vitreoretinopathy |
| url | http://dx.doi.org/10.1155/2013/857380 |
| work_keys_str_mv | AT claudioazzolini expressionofvegfaotxhomeoboxandp53familygenesinproliferativevitreoretinopathy AT ilariastefaniapagani expressionofvegfaotxhomeoboxandp53familygenesinproliferativevitreoretinopathy AT cristinapirrone expressionofvegfaotxhomeoboxandp53familygenesinproliferativevitreoretinopathy AT davideborroni expressionofvegfaotxhomeoboxandp53familygenesinproliferativevitreoretinopathy AT simonedonati expressionofvegfaotxhomeoboxandp53familygenesinproliferativevitreoretinopathy AT munaaloum expressionofvegfaotxhomeoboxandp53familygenesinproliferativevitreoretinopathy AT dianapigni expressionofvegfaotxhomeoboxandp53familygenesinproliferativevitreoretinopathy AT annamariachiaravalli expressionofvegfaotxhomeoboxandp53familygenesinproliferativevitreoretinopathy AT riccardovinciguerra expressionofvegfaotxhomeoboxandp53familygenesinproliferativevitreoretinopathy AT francescasimonelli expressionofvegfaotxhomeoboxandp53familygenesinproliferativevitreoretinopathy AT giovanniporta expressionofvegfaotxhomeoboxandp53familygenesinproliferativevitreoretinopathy |