Recognition Code of ZNF191(243-368) and Its Interaction with DNA
ZNF191(243-368) is the C-terminal region of ZNF191 which contains a putative DNA-binding domain of four Cys2His2 zinc finger motifs. In this study, an expression vector of a fusion protein of ZNF191(243-368) with glutathione-S-transferase (GST) was constructed and transformed into Escherichia coli B...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2015/416751 |
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| author | Dongxin Zhao Zhongxian Huang |
| author_facet | Dongxin Zhao Zhongxian Huang |
| author_sort | Dongxin Zhao |
| collection | DOAJ |
| description | ZNF191(243-368) is the C-terminal region of ZNF191 which contains a putative DNA-binding domain of four Cys2His2 zinc finger motifs. In this study, an expression vector of a fusion protein of ZNF191(243-368) with glutathione-S-transferase (GST) was constructed and transformed into Escherichia coli BL21. The fusion protein GST-ZNF191(243-368) was expressed using this vector to investigate the protein-DNA binding reaction through an affinity selection strategy on the basis of the binding quality of the zinc finger domain. Results showed that ZNF191(243-368) can selectively bind with sequences and react with genes which contain an AGGG core. However, the recognition mechanism of Cys2His2 zinc finger proteins to DNA warrants further investigation. |
| format | Article |
| id | doaj-art-b886b25f7c2b41b39dcc103305c2a266 |
| institution | Kabale University |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-b886b25f7c2b41b39dcc103305c2a2662025-02-03T01:11:26ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2015-01-01201510.1155/2015/416751416751Recognition Code of ZNF191(243-368) and Its Interaction with DNADongxin Zhao0Zhongxian Huang1School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, ChinaDepartment of Chemistry, Fudan University, Shanghai 200433, ChinaZNF191(243-368) is the C-terminal region of ZNF191 which contains a putative DNA-binding domain of four Cys2His2 zinc finger motifs. In this study, an expression vector of a fusion protein of ZNF191(243-368) with glutathione-S-transferase (GST) was constructed and transformed into Escherichia coli BL21. The fusion protein GST-ZNF191(243-368) was expressed using this vector to investigate the protein-DNA binding reaction through an affinity selection strategy on the basis of the binding quality of the zinc finger domain. Results showed that ZNF191(243-368) can selectively bind with sequences and react with genes which contain an AGGG core. However, the recognition mechanism of Cys2His2 zinc finger proteins to DNA warrants further investigation.http://dx.doi.org/10.1155/2015/416751 |
| spellingShingle | Dongxin Zhao Zhongxian Huang Recognition Code of ZNF191(243-368) and Its Interaction with DNA Bioinorganic Chemistry and Applications |
| title | Recognition Code of ZNF191(243-368) and Its Interaction with DNA |
| title_full | Recognition Code of ZNF191(243-368) and Its Interaction with DNA |
| title_fullStr | Recognition Code of ZNF191(243-368) and Its Interaction with DNA |
| title_full_unstemmed | Recognition Code of ZNF191(243-368) and Its Interaction with DNA |
| title_short | Recognition Code of ZNF191(243-368) and Its Interaction with DNA |
| title_sort | recognition code of znf191 243 368 and its interaction with dna |
| url | http://dx.doi.org/10.1155/2015/416751 |
| work_keys_str_mv | AT dongxinzhao recognitioncodeofznf191243368anditsinteractionwithdna AT zhongxianhuang recognitioncodeofznf191243368anditsinteractionwithdna |