Role of CFT and PCR in diagnosis of Chlamydia psittaci in experimentally infected rabbits
Specific antibodies against сhlamydia weredetectedusingcomplement fixation test and сhlamydiagenome wasdetectedusingpolymerase chain reaction in pregnant rabbits experimentally infected with Chlamydiapsittaci. The infectedrabbitsdevelopeda fever andrespiratory signs andthe infection was confirmed by...
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Da Vinci Media
2023-09-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/742 |
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| author | V. V. Evstifeev F. M. Khusainov S. I. Yakovlev R. I. Shangaraev V. I. Eremets |
| author_facet | V. V. Evstifeev F. M. Khusainov S. I. Yakovlev R. I. Shangaraev V. I. Eremets |
| author_sort | V. V. Evstifeev |
| collection | DOAJ |
| description | Specific antibodies against сhlamydia weredetectedusingcomplement fixation test and сhlamydiagenome wasdetectedusingpolymerase chain reaction in pregnant rabbits experimentally infected with Chlamydiapsittaci. The infectedrabbitsdevelopeda fever andrespiratory signs andthe infection was confirmed by specificantibodiesagainst сhlamydiadetectedin theirbloodandbyabnormalities in rabbitkindling. Complement fixation testofpairedrabbit serarevealed an increasein thetitersof specificantibodiesagainst сhlamydia, which on Day 7 post infection varied within 1:7.5; on Day 14, mean concentration was 1:40 and by Day 30 mean titer increasedto 1:60. However, when pathological materials from theurogenital tractof theexperimentalanimals weretestedin polymerase chain reaction andin smear microscopy, it was impossibletoconfirm that thereisan etiological linkbetween сhlamydiaandkindlingproblems in experimental animals. At thesametime, molecularandgenetictestsof internalorgans (liver) sampledfromstillborn babyrabbitsrevealedthe сhlamydiagenome, thus, proving сhlamydia involvement into thepathological kindling. Therefore, such a retrospective methodas complement fixation test with a сhlamydia antigen is of high diagnosticvaluefor lifetime сhlamydiadiagnosis. |
| format | Article |
| id | doaj-art-b86719fad2a641fdb10c1726e04b450e |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-b86719fad2a641fdb10c1726e04b450e2025-02-06T09:52:10ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592023-09-0112322222710.29326/2304-196X-2023-12-3-222-227625Role of CFT and PCR in diagnosis of Chlamydia psittaci in experimentally infected rabbitsV. V. Evstifeev0F. M. Khusainov1S. I. Yakovlev2R. I. Shangaraev3V. I. Eremets4FSBSI “Federal Center for Toxicological, Radiation, and Biological Safety” (FSBSI “FCTRBS-ARRVI”); FSBEI HE “Kazan State Academy of Veterinary Medicine by N. E. Bauman” (FSBEI HE Kazan SAVM)FSBSI “Federal Center for Toxicological, Radiation, and Biological Safety” (FSBSI “FCTRBS-ARRVI”)FSBSI “Federal Center for Toxicological, Radiation, and Biological Safety” (FSBSI “FCTRBS-ARRVI”)FSBI “All-Russian Research and Technological Institute of Biological Industry” (VNITIBP)ФГБНУ «Всероссийский научно-исследовательский и технологический институт биологической промышленности» (ФГБНУ ВНИТИБП)Specific antibodies against сhlamydia weredetectedusingcomplement fixation test and сhlamydiagenome wasdetectedusingpolymerase chain reaction in pregnant rabbits experimentally infected with Chlamydiapsittaci. The infectedrabbitsdevelopeda fever andrespiratory signs andthe infection was confirmed by specificantibodiesagainst сhlamydiadetectedin theirbloodandbyabnormalities in rabbitkindling. Complement fixation testofpairedrabbit serarevealed an increasein thetitersof specificantibodiesagainst сhlamydia, which on Day 7 post infection varied within 1:7.5; on Day 14, mean concentration was 1:40 and by Day 30 mean titer increasedto 1:60. However, when pathological materials from theurogenital tractof theexperimentalanimals weretestedin polymerase chain reaction andin smear microscopy, it was impossibletoconfirm that thereisan etiological linkbetween сhlamydiaandkindlingproblems in experimental animals. At thesametime, molecularandgenetictestsof internalorgans (liver) sampledfromstillborn babyrabbitsrevealedthe сhlamydiagenome, thus, proving сhlamydia involvement into thepathological kindling. Therefore, such a retrospective methodas complement fixation test with a сhlamydia antigen is of high diagnosticvaluefor lifetime сhlamydiadiagnosis.https://veterinary.arriah.ru/jour/article/view/742chlamydiosischlamydia psittacipolymerase chain reactionrabbitsserological tests |
| spellingShingle | V. V. Evstifeev F. M. Khusainov S. I. Yakovlev R. I. Shangaraev V. I. Eremets Role of CFT and PCR in diagnosis of Chlamydia psittaci in experimentally infected rabbits Ветеринария сегодня chlamydiosis chlamydia psittaci polymerase chain reaction rabbits serological tests |
| title | Role of CFT and PCR in diagnosis of Chlamydia psittaci in experimentally infected rabbits |
| title_full | Role of CFT and PCR in diagnosis of Chlamydia psittaci in experimentally infected rabbits |
| title_fullStr | Role of CFT and PCR in diagnosis of Chlamydia psittaci in experimentally infected rabbits |
| title_full_unstemmed | Role of CFT and PCR in diagnosis of Chlamydia psittaci in experimentally infected rabbits |
| title_short | Role of CFT and PCR in diagnosis of Chlamydia psittaci in experimentally infected rabbits |
| title_sort | role of cft and pcr in diagnosis of chlamydia psittaci in experimentally infected rabbits |
| topic | chlamydiosis chlamydia psittaci polymerase chain reaction rabbits serological tests |
| url | https://veterinary.arriah.ru/jour/article/view/742 |
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