In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulations
The aim of the study was to examine the effect of topical vehicles on the in vivo human stratum corneum penetration of the antioxidant and skin photoprotective agent (-)-epigallocatechin-3-gallate (EGCG). Model oil-in-water (o/w) emulsion and gel formulations containing 1 % (m/m) EGCG were prepared...
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| Format: | Article |
| Language: | English |
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Sciendo
2014-06-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | http://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0017/acph-2014-0017.xml?format=INT |
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| author | Scalia Santo Trotta Valentina Bianchi Anna |
| author_facet | Scalia Santo Trotta Valentina Bianchi Anna |
| author_sort | Scalia Santo |
| collection | DOAJ |
| description | The aim of the study was to examine the effect of topical vehicles on the in vivo human stratum corneum penetration of the antioxidant and skin photoprotective agent (-)-epigallocatechin-3-gallate (EGCG). Model oil-in-water (o/w) emulsion and gel formulations containing 1 % (m/m) EGCG were prepared and subjected to photodegradation studies in order to select excipients that minimize the light instability of EGCG. The optimized emulsion and gel were applied to human volunteers and the EGCG percutaneous permeation was evaluated in vivo by the tape- -stripping technique. No significant differences in the percentage of the applied EGCG dose diffused into the stratum corneum were observed between the o/w emulsion (36.1 ± 7.5 %) and gel (35.5 ± 8.1 %) preparations. However, the amount of EGCG permeated into the deeper region of human stratum corneum was significantly larger for the o/w emulsion compared to the gel. Therefore, the emulsion represents a suitable vehicle for topical delivery of EGCG. |
| format | Article |
| id | doaj-art-b851145469314bb3affef56e24a9e260 |
| institution | Kabale University |
| issn | 1330-0075 1846-9558 |
| language | English |
| publishDate | 2014-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-b851145469314bb3affef56e24a9e2602025-02-02T20:50:34ZengSciendoActa Pharmaceutica1330-00751846-95582014-06-0164225726510.2478/acph-2014-0017acph-2014-0017In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulationsScalia Santo0Trotta Valentina1Bianchi Anna2Department of Chemical and Pharmaceutical Sciences, University of Ferrara 44121 Ferrara, ItalyDepartment of Chemical and Pharmaceutical Sciences, University of Ferrara 44121 Ferrara, ItalyDepartment of Chemical and Pharmaceutical Sciences, University of Ferrara 44121 Ferrara, ItalyThe aim of the study was to examine the effect of topical vehicles on the in vivo human stratum corneum penetration of the antioxidant and skin photoprotective agent (-)-epigallocatechin-3-gallate (EGCG). Model oil-in-water (o/w) emulsion and gel formulations containing 1 % (m/m) EGCG were prepared and subjected to photodegradation studies in order to select excipients that minimize the light instability of EGCG. The optimized emulsion and gel were applied to human volunteers and the EGCG percutaneous permeation was evaluated in vivo by the tape- -stripping technique. No significant differences in the percentage of the applied EGCG dose diffused into the stratum corneum were observed between the o/w emulsion (36.1 ± 7.5 %) and gel (35.5 ± 8.1 %) preparations. However, the amount of EGCG permeated into the deeper region of human stratum corneum was significantly larger for the o/w emulsion compared to the gel. Therefore, the emulsion represents a suitable vehicle for topical delivery of EGCG.http://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0017/acph-2014-0017.xml?format=INT(-)-epigallocatechin-3-gallatetopical applicationin vivo human studytape-strippingemulsiongel |
| spellingShingle | Scalia Santo Trotta Valentina Bianchi Anna In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulations Acta Pharmaceutica (-)-epigallocatechin-3-gallate topical application in vivo human study tape-stripping emulsion gel |
| title | In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulations |
| title_full | In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulations |
| title_fullStr | In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulations |
| title_full_unstemmed | In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulations |
| title_short | In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulations |
| title_sort | in vivo human skin penetration of epigallocatechin 3 gallate from topical formulations |
| topic | (-)-epigallocatechin-3-gallate topical application in vivo human study tape-stripping emulsion gel |
| url | http://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0017/acph-2014-0017.xml?format=INT |
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