Deletion of the waaf gene affects O antigen synthesis and pathogenicity in Vibrio parahaemolyticus from shellfish
ibrio parahaemolyticus is the main cause of foodborne gastroenteritis, which is widely distributed in shellfish and other seafood. Most V. parahaemolyticus are nonpathogenic, and only a few types, such as serotype O3:K6, are pathogenic, which is also the most prevalent strain in Asia. However, the r...
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Tsinghua University Press
2022-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021001233 |
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| author | Feng Zhao Guoying Ding Qilong Wang Huihui Du Guosheng Xiao Deqing Zhou |
| author_facet | Feng Zhao Guoying Ding Qilong Wang Huihui Du Guosheng Xiao Deqing Zhou |
| author_sort | Feng Zhao |
| collection | DOAJ |
| description | ibrio parahaemolyticus is the main cause of foodborne gastroenteritis, which is widely distributed in shellfish and other seafood. Most V. parahaemolyticus are nonpathogenic, and only a few types, such as serotype O3:K6, are pathogenic, which is also the most prevalent strain in Asia. However, the relationship between this serotype and pathogenicity has yet to be established. The waaf gene is located in the O antigen synthesis gene cluster. Thus, we constructed a waaf gene deletion mutant (i.e., △waaf) of wild-type (WT) which isolated from shellfish serotype O3:K6 via chitin-mediated transformation technology. We then constructed the △waaf complementary strain (i.e., C-Δwaaf) via the Escherichia coli S17 λpir strain by conjugation. The basic physiological characteristics, adhesion to Caco2 cells, and pathogenicity of the WT, Δwaaf, and C-Δwaaf strains were compared. Growth curves showed no remarkable differences between the WT and Δwaaf strains. However, the Δwaaf strain non-reactive to O3 antisera and other 12 O-group antisera of V. parahaemolyticus. Moreover, the number of flagella and extracellular polysaccharides decreased, the adhesion decreased, and the pathogenicity weakened. These characteristics of the C-Δwaaf strain were similar to those of the WT strain. These results indicated that the waaf gene is vital to the serotype in V. parahaemolyticus, and changes in O antigen could affect the pathogenicity of this bacterium. This study will be helpful to understand the pathogenic mechanism of V. parahaemolyticus. |
| format | Article |
| id | doaj-art-b8471467dd794ccb84bd70694ebe06f1 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-b8471467dd794ccb84bd70694ebe06f12025-02-03T06:48:59ZengTsinghua University PressFood Science and Human Wellness2213-45302022-03-01112418426Deletion of the waaf gene affects O antigen synthesis and pathogenicity in Vibrio parahaemolyticus from shellfishFeng Zhao0Guoying Ding1Qilong Wang2Huihui Du3Guosheng Xiao4Deqing Zhou5College of biology and food engineering, Chongqing Three Gorges University, Wanzhou 404100, ChinaCollege of biology and food engineering, Chongqing Three Gorges University, Wanzhou 404100, ChinaCollege of biology and food engineering, Chongqing Three Gorges University, Wanzhou 404100, ChinaCollege of biology and food engineering, Chongqing Three Gorges University, Wanzhou 404100, ChinaCollege of biology and food engineering, Chongqing Three Gorges University, Wanzhou 404100, China; Corresponding author.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Drugs and Bioproducts of Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071, China; Corresponding author.ibrio parahaemolyticus is the main cause of foodborne gastroenteritis, which is widely distributed in shellfish and other seafood. Most V. parahaemolyticus are nonpathogenic, and only a few types, such as serotype O3:K6, are pathogenic, which is also the most prevalent strain in Asia. However, the relationship between this serotype and pathogenicity has yet to be established. The waaf gene is located in the O antigen synthesis gene cluster. Thus, we constructed a waaf gene deletion mutant (i.e., △waaf) of wild-type (WT) which isolated from shellfish serotype O3:K6 via chitin-mediated transformation technology. We then constructed the △waaf complementary strain (i.e., C-Δwaaf) via the Escherichia coli S17 λpir strain by conjugation. The basic physiological characteristics, adhesion to Caco2 cells, and pathogenicity of the WT, Δwaaf, and C-Δwaaf strains were compared. Growth curves showed no remarkable differences between the WT and Δwaaf strains. However, the Δwaaf strain non-reactive to O3 antisera and other 12 O-group antisera of V. parahaemolyticus. Moreover, the number of flagella and extracellular polysaccharides decreased, the adhesion decreased, and the pathogenicity weakened. These characteristics of the C-Δwaaf strain were similar to those of the WT strain. These results indicated that the waaf gene is vital to the serotype in V. parahaemolyticus, and changes in O antigen could affect the pathogenicity of this bacterium. This study will be helpful to understand the pathogenic mechanism of V. parahaemolyticus.http://www.sciencedirect.com/science/article/pii/S2213453021001233Vibrio parahaemolyticusWaaf geneLPSO antigenPathogenicity |
| spellingShingle | Feng Zhao Guoying Ding Qilong Wang Huihui Du Guosheng Xiao Deqing Zhou Deletion of the waaf gene affects O antigen synthesis and pathogenicity in Vibrio parahaemolyticus from shellfish Food Science and Human Wellness Vibrio parahaemolyticus Waaf gene LPS O antigen Pathogenicity |
| title | Deletion of the waaf gene affects O antigen synthesis and pathogenicity in Vibrio parahaemolyticus from shellfish |
| title_full | Deletion of the waaf gene affects O antigen synthesis and pathogenicity in Vibrio parahaemolyticus from shellfish |
| title_fullStr | Deletion of the waaf gene affects O antigen synthesis and pathogenicity in Vibrio parahaemolyticus from shellfish |
| title_full_unstemmed | Deletion of the waaf gene affects O antigen synthesis and pathogenicity in Vibrio parahaemolyticus from shellfish |
| title_short | Deletion of the waaf gene affects O antigen synthesis and pathogenicity in Vibrio parahaemolyticus from shellfish |
| title_sort | deletion of the waaf gene affects o antigen synthesis and pathogenicity in vibrio parahaemolyticus from shellfish |
| topic | Vibrio parahaemolyticus Waaf gene LPS O antigen Pathogenicity |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021001233 |
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