Transcriptomic analysis of melatonin on the mechanism of embryonic gonadal development in female Jilin white geese
The goose industry represents a significant sector within the broader waterfowl industry in China, with early gonadal development playing a pivotal role in enhancing population productivity. Melatonin plays a crucial role in regulating early gonadal development, but the molecular mechanism underlyin...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011052 |
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| author | Jin Yu Ichraf Mabrouk Qiuyuan Liu Yuxuan Zhou Yupu Song Jingyun Ma Fengshuo Liu Xiangman Hu Zhiyi Yang Yuxia Zeng Jingtao Hu Yongfeng Sun |
| author_facet | Jin Yu Ichraf Mabrouk Qiuyuan Liu Yuxuan Zhou Yupu Song Jingyun Ma Fengshuo Liu Xiangman Hu Zhiyi Yang Yuxia Zeng Jingtao Hu Yongfeng Sun |
| author_sort | Jin Yu |
| collection | DOAJ |
| description | The goose industry represents a significant sector within the broader waterfowl industry in China, with early gonadal development playing a pivotal role in enhancing population productivity. Melatonin plays a crucial role in regulating early gonadal development, but the molecular mechanism underlying the effect of melatonin as a regulator remains unclear. In this study, the relationship between melatonin and egg production performance in Jilin white goose was first established, then, an in Ovo injection of melatonin in goose embryos on the embryonic day 12 (E12) was performed to evaluate the impact of melatonin on the gonadal development in Jilin white goose. The results showed that the in Ovo injection of melatonin increased the expression of FOXL2 in the gonads while decreasing the expression of CYP19A1. Transcriptome analyses revealed that a total of 1184 differentially expressed genes were screened in Jilin white goose embryonic gonads among the two comparison groups. These DEGs were enriched in Gene Ontology (GO) terms related to the microtubule cytoskeleton, cytoskeleton, cell adhesion, and biological adhesion and the KEGG enrichment analysis suggested that the DEGs were mainly enriched in “MAPK signaling pathway”, “Wnt signaling pathway”, “Calcium signaling pathway”, “Focal adhesion”, “Neuroactive ligand-receptor interaction”, “Melanogenesis”, “mTOR signaling pathway”, “ECM-receptor interaction”, and “TGF-beta signaling pathway” in the two comparison groups. The protein-protein interaction network analysis (PPI) indicated that selected DEGs, such as PDIA3, ATCB, PKM, PGAM1, NME2, LDHB, and CALM2, were highly related to the regulation of the reproduction system development. Additionally, the expression trends of 4 identified DEGs were validated by RT-qPCR. In conclusion, this study elucidates the potential regulatory role of melatonin in gonadal development in geese, identifying candidate genes involved in early gonadal differentiation and maturation. Furthermore, the findings offer valuable practical implications for the goose industry, providing professionals with scientifically grounded strategies to enhance reproductive efficiency and overall population productivity. |
| format | Article |
| id | doaj-art-b8408d426f0445cb9a5a0ed93e43c156 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-b8408d426f0445cb9a5a0ed93e43c1562025-01-22T05:40:21ZengElsevierPoultry Science0032-57912025-01-011041104527Transcriptomic analysis of melatonin on the mechanism of embryonic gonadal development in female Jilin white geeseJin Yu0Ichraf Mabrouk1Qiuyuan Liu2Yuxuan Zhou3Yupu Song4Jingyun Ma5Fengshuo Liu6Xiangman Hu7Zhiyi Yang8Yuxia Zeng9Jingtao Hu10Yongfeng Sun11College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China; Corresponding author.The goose industry represents a significant sector within the broader waterfowl industry in China, with early gonadal development playing a pivotal role in enhancing population productivity. Melatonin plays a crucial role in regulating early gonadal development, but the molecular mechanism underlying the effect of melatonin as a regulator remains unclear. In this study, the relationship between melatonin and egg production performance in Jilin white goose was first established, then, an in Ovo injection of melatonin in goose embryos on the embryonic day 12 (E12) was performed to evaluate the impact of melatonin on the gonadal development in Jilin white goose. The results showed that the in Ovo injection of melatonin increased the expression of FOXL2 in the gonads while decreasing the expression of CYP19A1. Transcriptome analyses revealed that a total of 1184 differentially expressed genes were screened in Jilin white goose embryonic gonads among the two comparison groups. These DEGs were enriched in Gene Ontology (GO) terms related to the microtubule cytoskeleton, cytoskeleton, cell adhesion, and biological adhesion and the KEGG enrichment analysis suggested that the DEGs were mainly enriched in “MAPK signaling pathway”, “Wnt signaling pathway”, “Calcium signaling pathway”, “Focal adhesion”, “Neuroactive ligand-receptor interaction”, “Melanogenesis”, “mTOR signaling pathway”, “ECM-receptor interaction”, and “TGF-beta signaling pathway” in the two comparison groups. The protein-protein interaction network analysis (PPI) indicated that selected DEGs, such as PDIA3, ATCB, PKM, PGAM1, NME2, LDHB, and CALM2, were highly related to the regulation of the reproduction system development. Additionally, the expression trends of 4 identified DEGs were validated by RT-qPCR. In conclusion, this study elucidates the potential regulatory role of melatonin in gonadal development in geese, identifying candidate genes involved in early gonadal differentiation and maturation. Furthermore, the findings offer valuable practical implications for the goose industry, providing professionals with scientifically grounded strategies to enhance reproductive efficiency and overall population productivity.http://www.sciencedirect.com/science/article/pii/S0032579124011052MelatoninIn Ovo injectionGonadal developmentTranscriptomics |
| spellingShingle | Jin Yu Ichraf Mabrouk Qiuyuan Liu Yuxuan Zhou Yupu Song Jingyun Ma Fengshuo Liu Xiangman Hu Zhiyi Yang Yuxia Zeng Jingtao Hu Yongfeng Sun Transcriptomic analysis of melatonin on the mechanism of embryonic gonadal development in female Jilin white geese Poultry Science Melatonin In Ovo injection Gonadal development Transcriptomics |
| title | Transcriptomic analysis of melatonin on the mechanism of embryonic gonadal development in female Jilin white geese |
| title_full | Transcriptomic analysis of melatonin on the mechanism of embryonic gonadal development in female Jilin white geese |
| title_fullStr | Transcriptomic analysis of melatonin on the mechanism of embryonic gonadal development in female Jilin white geese |
| title_full_unstemmed | Transcriptomic analysis of melatonin on the mechanism of embryonic gonadal development in female Jilin white geese |
| title_short | Transcriptomic analysis of melatonin on the mechanism of embryonic gonadal development in female Jilin white geese |
| title_sort | transcriptomic analysis of melatonin on the mechanism of embryonic gonadal development in female jilin white geese |
| topic | Melatonin In Ovo injection Gonadal development Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011052 |
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