Food nutrition and toxicology targeting on specific organs in the era of single-cell sequencing
Due to the complex natures of dietary food components, it is difficult to elucidate how the compounds affect host health. Dietary food often selectively presents its mechanism of action on different cell types, and participates in the modulation of targeted cells and their microenvironments within o...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2024-01-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023001544 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832553420453576704 |
|---|---|
| author | Xiaofei Wang Xiaowen Cheng Huiling Liu Xiaohuan Mu Hao Zheng |
| author_facet | Xiaofei Wang Xiaowen Cheng Huiling Liu Xiaohuan Mu Hao Zheng |
| author_sort | Xiaofei Wang |
| collection | DOAJ |
| description | Due to the complex natures of dietary food components, it is difficult to elucidate how the compounds affect host health. Dietary food often selectively presents its mechanism of action on different cell types, and participates in the modulation of targeted cells and their microenvironments within organs. However, the limitations of traditional in vitro assays or in vivo animal experiments cannot comprehensively examine cellular heterogeneity and the tissue-biased influences. Single-cell RNA sequencing (scRNA-seq) has emerged as an indispensable methodology to decompose tissues into different cell types for the demonstration of transcriptional profiles of individual cells. ScRNA-seq applications has been summarized on three typical organs (brain, liver, kidney), and two representative immune- and tumor related health problems. The ever-increasing role of scRNA-seq in dietary food research with further improvement can provide sub-cellular information and the coupling between other cellular modalities. In this review, we propose utilizing scRNA-seq to more effectively capture the subtle and complex effects of food chemicals, and how they may lead to health problems at single-cell resolution. This novel technique will be valuable to elucidate the underlying mechanism of both the health benefits of food nutrients and the detrimental consequences food toxicants at the cellular level. |
| format | Article |
| id | doaj-art-b832f25402b746e1907ad741074d826f |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-b832f25402b746e1907ad741074d826f2025-02-03T05:54:06ZengTsinghua University PressFood Science and Human Wellness2213-45302024-01-011317589Food nutrition and toxicology targeting on specific organs in the era of single-cell sequencingXiaofei Wang0Xiaowen Cheng1Huiling Liu2Xiaohuan Mu3Hao Zheng4National Engineering Technology Research Center for Fruit and Vegetable Processing, Key Open Laboratory of Fruit and Vegetable Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Food Non-Thermal Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaNational Engineering Technology Research Center for Fruit and Vegetable Processing, Key Open Laboratory of Fruit and Vegetable Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Food Non-Thermal Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaNational Engineering Technology Research Center for Fruit and Vegetable Processing, Key Open Laboratory of Fruit and Vegetable Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Food Non-Thermal Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaNational Engineering Technology Research Center for Fruit and Vegetable Processing, Key Open Laboratory of Fruit and Vegetable Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Food Non-Thermal Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCorresponding author at: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.; National Engineering Technology Research Center for Fruit and Vegetable Processing, Key Open Laboratory of Fruit and Vegetable Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Food Non-Thermal Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaDue to the complex natures of dietary food components, it is difficult to elucidate how the compounds affect host health. Dietary food often selectively presents its mechanism of action on different cell types, and participates in the modulation of targeted cells and their microenvironments within organs. However, the limitations of traditional in vitro assays or in vivo animal experiments cannot comprehensively examine cellular heterogeneity and the tissue-biased influences. Single-cell RNA sequencing (scRNA-seq) has emerged as an indispensable methodology to decompose tissues into different cell types for the demonstration of transcriptional profiles of individual cells. ScRNA-seq applications has been summarized on three typical organs (brain, liver, kidney), and two representative immune- and tumor related health problems. The ever-increasing role of scRNA-seq in dietary food research with further improvement can provide sub-cellular information and the coupling between other cellular modalities. In this review, we propose utilizing scRNA-seq to more effectively capture the subtle and complex effects of food chemicals, and how they may lead to health problems at single-cell resolution. This novel technique will be valuable to elucidate the underlying mechanism of both the health benefits of food nutrients and the detrimental consequences food toxicants at the cellular level.http://www.sciencedirect.com/science/article/pii/S2213453023001544Dietary foodCellular heterogeneitySingle-cell RNA sequencingFood nutrientsFood toxicants |
| spellingShingle | Xiaofei Wang Xiaowen Cheng Huiling Liu Xiaohuan Mu Hao Zheng Food nutrition and toxicology targeting on specific organs in the era of single-cell sequencing Food Science and Human Wellness Dietary food Cellular heterogeneity Single-cell RNA sequencing Food nutrients Food toxicants |
| title | Food nutrition and toxicology targeting on specific organs in the era of single-cell sequencing |
| title_full | Food nutrition and toxicology targeting on specific organs in the era of single-cell sequencing |
| title_fullStr | Food nutrition and toxicology targeting on specific organs in the era of single-cell sequencing |
| title_full_unstemmed | Food nutrition and toxicology targeting on specific organs in the era of single-cell sequencing |
| title_short | Food nutrition and toxicology targeting on specific organs in the era of single-cell sequencing |
| title_sort | food nutrition and toxicology targeting on specific organs in the era of single cell sequencing |
| topic | Dietary food Cellular heterogeneity Single-cell RNA sequencing Food nutrients Food toxicants |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023001544 |
| work_keys_str_mv | AT xiaofeiwang foodnutritionandtoxicologytargetingonspecificorgansintheeraofsinglecellsequencing AT xiaowencheng foodnutritionandtoxicologytargetingonspecificorgansintheeraofsinglecellsequencing AT huilingliu foodnutritionandtoxicologytargetingonspecificorgansintheeraofsinglecellsequencing AT xiaohuanmu foodnutritionandtoxicologytargetingonspecificorgansintheeraofsinglecellsequencing AT haozheng foodnutritionandtoxicologytargetingonspecificorgansintheeraofsinglecellsequencing |