Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration
Axonal injuries at the interface between central and peripheral nervous system, such as ventral root avulsion (VRA), induce important degenerative processes, mostly resulting in neuronal and motor function loss. In the present work, we have compared two different fibrin sealants, one derived from hu...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2016/2932784 |
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| author | Mateus Vidigal de Castro Roberta Barbizan Rui Seabra Ferreira Benedito Barraviera Alexandre Leite Rodrigues de Oliveira |
| author_facet | Mateus Vidigal de Castro Roberta Barbizan Rui Seabra Ferreira Benedito Barraviera Alexandre Leite Rodrigues de Oliveira |
| author_sort | Mateus Vidigal de Castro |
| collection | DOAJ |
| description | Axonal injuries at the interface between central and peripheral nervous system, such as ventral root avulsion (VRA), induce important degenerative processes, mostly resulting in neuronal and motor function loss. In the present work, we have compared two different fibrin sealants, one derived from human blood and another derived from animal blood and Crotalus durissus terrificus venom, as a promising treatment for this type of injury. Lewis rats were submitted to VRA (L4–L6) and had the avulsed roots reimplanted to the surface of the spinal cord, with the aid of fibrin sealant. The spinal cords were processed to evaluate neuronal survival, synaptic stability, and glial reactivity, 4 and 12 weeks after lesion. Sciatic nerves were processed to investigate Schwann cell activity by p75NTR expression (4 weeks after surgery) and to count myelinated axons and morphometric evaluation (12 weeks after surgery). Walking track test was used to evaluate gait recovery, up to 12 weeks. The results indicate that both fibrin sealants are similarly efficient. However, the snake-derived fibrin glue is a potentially safer alternative for being a biological and biodegradable product which does not contain human blood derivatives. Therefore, the venom glue can be a useful tool for the scientific community due to its advantages and variety of applications. |
| format | Article |
| id | doaj-art-b8295218db754f4bbb84c99eb5518187 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-b8295218db754f4bbb84c99eb55181872025-02-03T05:43:34ZengWileyNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/29327842932784Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and RegenerationMateus Vidigal de Castro0Roberta Barbizan1Rui Seabra Ferreira2Benedito Barraviera3Alexandre Leite Rodrigues de Oliveira4Department of Structural and Functional Biology, Institute of Biology, University of Campinas, 13083-030 Campinas, SP, BrazilDepartment of Structural and Functional Biology, Institute of Biology, University of Campinas, 13083-030 Campinas, SP, BrazilDepartment of Tropical Diseases, Botucatu Medical School, São Paulo State University (UNESP), 18618-000 Botucatu, SP, BrazilDepartment of Tropical Diseases, Botucatu Medical School, São Paulo State University (UNESP), 18618-000 Botucatu, SP, BrazilDepartment of Structural and Functional Biology, Institute of Biology, University of Campinas, 13083-030 Campinas, SP, BrazilAxonal injuries at the interface between central and peripheral nervous system, such as ventral root avulsion (VRA), induce important degenerative processes, mostly resulting in neuronal and motor function loss. In the present work, we have compared two different fibrin sealants, one derived from human blood and another derived from animal blood and Crotalus durissus terrificus venom, as a promising treatment for this type of injury. Lewis rats were submitted to VRA (L4–L6) and had the avulsed roots reimplanted to the surface of the spinal cord, with the aid of fibrin sealant. The spinal cords were processed to evaluate neuronal survival, synaptic stability, and glial reactivity, 4 and 12 weeks after lesion. Sciatic nerves were processed to investigate Schwann cell activity by p75NTR expression (4 weeks after surgery) and to count myelinated axons and morphometric evaluation (12 weeks after surgery). Walking track test was used to evaluate gait recovery, up to 12 weeks. The results indicate that both fibrin sealants are similarly efficient. However, the snake-derived fibrin glue is a potentially safer alternative for being a biological and biodegradable product which does not contain human blood derivatives. Therefore, the venom glue can be a useful tool for the scientific community due to its advantages and variety of applications.http://dx.doi.org/10.1155/2016/2932784 |
| spellingShingle | Mateus Vidigal de Castro Roberta Barbizan Rui Seabra Ferreira Benedito Barraviera Alexandre Leite Rodrigues de Oliveira Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration Neural Plasticity |
| title | Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration |
| title_full | Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration |
| title_fullStr | Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration |
| title_full_unstemmed | Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration |
| title_short | Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration |
| title_sort | direct spinal ventral root repair following avulsion effectiveness of a new heterologous fibrin sealant on motoneuron survival and regeneration |
| url | http://dx.doi.org/10.1155/2016/2932784 |
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