α-Copaene is a potent repellent against the Asian Citrus Psyllid Diaphorina citri
Abstract The Asian Citrus Psyllid (ACP), Diaphorina citri, severely threatens citrus production worldwide by transmitting the greening (= Huanglongbing)—causing bacterium Candidatus Liberibacter asiaticus. There is growing evidence that the push-pull strategy is suitable to partially mitigate HLB by...
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| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-86369-1 |
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| author | Rodrigo Facchini Magnani Haroldo Xavier Linhares Volpe Rejane Angélica Grigio Luvizotto Tatiana Aparecida Mulinari Thiago Trevisoli Agostini Jairo Kenupp Bastos Victor Pena Ribeiro Michele Carmo-Sousa Nelson Arno Wulff Leandro Peña Walter S. Leal |
| author_facet | Rodrigo Facchini Magnani Haroldo Xavier Linhares Volpe Rejane Angélica Grigio Luvizotto Tatiana Aparecida Mulinari Thiago Trevisoli Agostini Jairo Kenupp Bastos Victor Pena Ribeiro Michele Carmo-Sousa Nelson Arno Wulff Leandro Peña Walter S. Leal |
| author_sort | Rodrigo Facchini Magnani |
| collection | DOAJ |
| description | Abstract The Asian Citrus Psyllid (ACP), Diaphorina citri, severely threatens citrus production worldwide by transmitting the greening (= Huanglongbing)—causing bacterium Candidatus Liberibacter asiaticus. There is growing evidence that the push-pull strategy is suitable to partially mitigate HLB by repelling ACP with transgenic plants engineered to produce repellents and attracting the vector to plants with a minimal disease transmission rate. Species that pull ACP away from commercial citrus plants have been identified, and transgenic plants that repel ACP have been developed. The concept of a repellent-producing plant was first demonstrated with an Arabidopsis line engineered to overexpress a gene controlling the synthesis of β-caryophyllene and other sesquiterpenes. We have analyzed the volatile organic compounds released by this Arabidopsis line and identified α-humulene, α-copaene, and trace amounts of β-elemene, in addition to β-caryophyllene. Behavioral measurements demonstrated that α-copaene repels ACP at doses ca. 100× lower than those needed for β-caryophyllene repellence. In contrast, α-humulene is innocuous at the level emitted by the transgenic plant. We confirmed that a mixture of the three sesquiterpenes in the ratio 1:100:10 repels ACP. Likewise, a commercial sample of copaiba oil containing the three sesquiterpenes, in a proportion similar to that in the transgenic plant, repelled ACP. |
| format | Article |
| id | doaj-art-b81282bac75c43a48995928936eedfa0 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-b81282bac75c43a48995928936eedfa02025-02-02T12:17:03ZengNature PortfolioScientific Reports2045-23222025-01-0115111010.1038/s41598-025-86369-1α-Copaene is a potent repellent against the Asian Citrus Psyllid Diaphorina citriRodrigo Facchini Magnani0Haroldo Xavier Linhares Volpe1Rejane Angélica Grigio Luvizotto2Tatiana Aparecida Mulinari3Thiago Trevisoli Agostini4Jairo Kenupp Bastos5Victor Pena Ribeiro6Michele Carmo-Sousa7Nelson Arno Wulff8Leandro Peña9Walter S. Leal10Department of Research and Development, Fund for Citrus Protection (Fundecitrus)Department of Research and Development, Fund for Citrus Protection (Fundecitrus)Department of Research and Development, Fund for Citrus Protection (Fundecitrus)Department of Research and Development, Fund for Citrus Protection (Fundecitrus)Department of Research and Development, Fund for Citrus Protection (Fundecitrus)School of Pharmaceutical Sciences, University of São Paulo (USP)School of Pharmaceutical Sciences, University of São Paulo (USP)Department of Research and Development, Fund for Citrus Protection (Fundecitrus)Department of Research and Development, Fund for Citrus Protection (Fundecitrus)Department of Research and Development, Fund for Citrus Protection (Fundecitrus)Department of Molecular and Cellular Biology, University of California-DavisAbstract The Asian Citrus Psyllid (ACP), Diaphorina citri, severely threatens citrus production worldwide by transmitting the greening (= Huanglongbing)—causing bacterium Candidatus Liberibacter asiaticus. There is growing evidence that the push-pull strategy is suitable to partially mitigate HLB by repelling ACP with transgenic plants engineered to produce repellents and attracting the vector to plants with a minimal disease transmission rate. Species that pull ACP away from commercial citrus plants have been identified, and transgenic plants that repel ACP have been developed. The concept of a repellent-producing plant was first demonstrated with an Arabidopsis line engineered to overexpress a gene controlling the synthesis of β-caryophyllene and other sesquiterpenes. We have analyzed the volatile organic compounds released by this Arabidopsis line and identified α-humulene, α-copaene, and trace amounts of β-elemene, in addition to β-caryophyllene. Behavioral measurements demonstrated that α-copaene repels ACP at doses ca. 100× lower than those needed for β-caryophyllene repellence. In contrast, α-humulene is innocuous at the level emitted by the transgenic plant. We confirmed that a mixture of the three sesquiterpenes in the ratio 1:100:10 repels ACP. Likewise, a commercial sample of copaiba oil containing the three sesquiterpenes, in a proportion similar to that in the transgenic plant, repelled ACP.https://doi.org/10.1038/s41598-025-86369-1β-caryophylleneα-humuleneα-copaeneCopaiba oilAt5g44630Repellent |
| spellingShingle | Rodrigo Facchini Magnani Haroldo Xavier Linhares Volpe Rejane Angélica Grigio Luvizotto Tatiana Aparecida Mulinari Thiago Trevisoli Agostini Jairo Kenupp Bastos Victor Pena Ribeiro Michele Carmo-Sousa Nelson Arno Wulff Leandro Peña Walter S. Leal α-Copaene is a potent repellent against the Asian Citrus Psyllid Diaphorina citri Scientific Reports β-caryophyllene α-humulene α-copaene Copaiba oil At5g44630 Repellent |
| title | α-Copaene is a potent repellent against the Asian Citrus Psyllid Diaphorina citri |
| title_full | α-Copaene is a potent repellent against the Asian Citrus Psyllid Diaphorina citri |
| title_fullStr | α-Copaene is a potent repellent against the Asian Citrus Psyllid Diaphorina citri |
| title_full_unstemmed | α-Copaene is a potent repellent against the Asian Citrus Psyllid Diaphorina citri |
| title_short | α-Copaene is a potent repellent against the Asian Citrus Psyllid Diaphorina citri |
| title_sort | α copaene is a potent repellent against the asian citrus psyllid diaphorina citri |
| topic | β-caryophyllene α-humulene α-copaene Copaiba oil At5g44630 Repellent |
| url | https://doi.org/10.1038/s41598-025-86369-1 |
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