Supplementation with Complex Phytonutrients Enhances Rumen Barrier Function and Growth Performance of Lambs by Regulating Rumen Microbiome and Metabolome
Complex phytonutrients (CPS) have attracted extensive interest due to their anti-inflammatory effects. This investigation focused on the impact of CPS on rumen health in lambs on high-concentrate diets, emphasizing growth performance, ruminal fermentation, epithelial barrier integrity, ruminal metab...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/15/2/228 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589351908802560 |
|---|---|
| author | Juan Du Yuan Wang Shaohui Su Wenwen Wang Tao Guo Yuchao Hu Na Yin Xiaoping An Jingwei Qi Xuan Xu |
| author_facet | Juan Du Yuan Wang Shaohui Su Wenwen Wang Tao Guo Yuchao Hu Na Yin Xiaoping An Jingwei Qi Xuan Xu |
| author_sort | Juan Du |
| collection | DOAJ |
| description | Complex phytonutrients (CPS) have attracted extensive interest due to their anti-inflammatory effects. This investigation focused on the impact of CPS on rumen health in lambs on high-concentrate diets, emphasizing growth performance, ruminal fermentation, epithelial barrier integrity, ruminal metabolism, and microbial communities. A total of 54 lambs, 3 months old and with a 30.42 ± 0.54 kg body weight, were randomly assigned to three treatment groups, with six replicates per treatment and three lambs per replicate. The lambs received a basal diet (the ratio of concentrate to forage was 75:25) without CPS supplementation (CON) or with the inclusion of 2.5 g/kg (CPS2.5) or 5.0 g/kg CPS (CPS5.0) for a total of 60 days. The CPS groups exhibited increased growth performance and improved rumen fermentation parameters. Mechanistically, CPS enhanced rumen epithelial barrier function, thereby lowering inflammation and inhibiting the overactivation of the JNK/p38 MAPK signaling pathway, and the effect of CPS5.0 was better than that of CPS2.5. Notably, CPS5.0 could optimize the composition of rumen microbiota and increase the levels of Ursolic acid and other metabolites. The strong associations between rumen bacteria and health-related indicators and differential metabolites were further highlighted. Our findings suggest that adding CPS to lambs’ diets has widespread positive impacts, including improved growth performance, reduced inflammation and mRNA relative expression of apoptosis-related genes, enhanced barrier function, and beneficial changes in the rumen microbiome and metabolite profiles. |
| format | Article |
| id | doaj-art-b7d31c35c08f40c386e7de88ed720701 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-b7d31c35c08f40c386e7de88ed7207012025-01-24T13:18:11ZengMDPI AGAnimals2076-26152025-01-0115222810.3390/ani15020228Supplementation with Complex Phytonutrients Enhances Rumen Barrier Function and Growth Performance of Lambs by Regulating Rumen Microbiome and MetabolomeJuan Du0Yuan Wang1Shaohui Su2Wenwen Wang3Tao Guo4Yuchao Hu5Na Yin6Xiaoping An7Jingwei Qi8Xuan Xu9College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaDepartment of Statistics, Kansas State University, Manhattan, KS 66061, USAComplex phytonutrients (CPS) have attracted extensive interest due to their anti-inflammatory effects. This investigation focused on the impact of CPS on rumen health in lambs on high-concentrate diets, emphasizing growth performance, ruminal fermentation, epithelial barrier integrity, ruminal metabolism, and microbial communities. A total of 54 lambs, 3 months old and with a 30.42 ± 0.54 kg body weight, were randomly assigned to three treatment groups, with six replicates per treatment and three lambs per replicate. The lambs received a basal diet (the ratio of concentrate to forage was 75:25) without CPS supplementation (CON) or with the inclusion of 2.5 g/kg (CPS2.5) or 5.0 g/kg CPS (CPS5.0) for a total of 60 days. The CPS groups exhibited increased growth performance and improved rumen fermentation parameters. Mechanistically, CPS enhanced rumen epithelial barrier function, thereby lowering inflammation and inhibiting the overactivation of the JNK/p38 MAPK signaling pathway, and the effect of CPS5.0 was better than that of CPS2.5. Notably, CPS5.0 could optimize the composition of rumen microbiota and increase the levels of Ursolic acid and other metabolites. The strong associations between rumen bacteria and health-related indicators and differential metabolites were further highlighted. Our findings suggest that adding CPS to lambs’ diets has widespread positive impacts, including improved growth performance, reduced inflammation and mRNA relative expression of apoptosis-related genes, enhanced barrier function, and beneficial changes in the rumen microbiome and metabolite profiles.https://www.mdpi.com/2076-2615/15/2/228complex phytonutrientshigh-concentrate dietrumen healthapoptosisinflammation |
| spellingShingle | Juan Du Yuan Wang Shaohui Su Wenwen Wang Tao Guo Yuchao Hu Na Yin Xiaoping An Jingwei Qi Xuan Xu Supplementation with Complex Phytonutrients Enhances Rumen Barrier Function and Growth Performance of Lambs by Regulating Rumen Microbiome and Metabolome Animals complex phytonutrients high-concentrate diet rumen health apoptosis inflammation |
| title | Supplementation with Complex Phytonutrients Enhances Rumen Barrier Function and Growth Performance of Lambs by Regulating Rumen Microbiome and Metabolome |
| title_full | Supplementation with Complex Phytonutrients Enhances Rumen Barrier Function and Growth Performance of Lambs by Regulating Rumen Microbiome and Metabolome |
| title_fullStr | Supplementation with Complex Phytonutrients Enhances Rumen Barrier Function and Growth Performance of Lambs by Regulating Rumen Microbiome and Metabolome |
| title_full_unstemmed | Supplementation with Complex Phytonutrients Enhances Rumen Barrier Function and Growth Performance of Lambs by Regulating Rumen Microbiome and Metabolome |
| title_short | Supplementation with Complex Phytonutrients Enhances Rumen Barrier Function and Growth Performance of Lambs by Regulating Rumen Microbiome and Metabolome |
| title_sort | supplementation with complex phytonutrients enhances rumen barrier function and growth performance of lambs by regulating rumen microbiome and metabolome |
| topic | complex phytonutrients high-concentrate diet rumen health apoptosis inflammation |
| url | https://www.mdpi.com/2076-2615/15/2/228 |
| work_keys_str_mv | AT juandu supplementationwithcomplexphytonutrientsenhancesrumenbarrierfunctionandgrowthperformanceoflambsbyregulatingrumenmicrobiomeandmetabolome AT yuanwang supplementationwithcomplexphytonutrientsenhancesrumenbarrierfunctionandgrowthperformanceoflambsbyregulatingrumenmicrobiomeandmetabolome AT shaohuisu supplementationwithcomplexphytonutrientsenhancesrumenbarrierfunctionandgrowthperformanceoflambsbyregulatingrumenmicrobiomeandmetabolome AT wenwenwang supplementationwithcomplexphytonutrientsenhancesrumenbarrierfunctionandgrowthperformanceoflambsbyregulatingrumenmicrobiomeandmetabolome AT taoguo supplementationwithcomplexphytonutrientsenhancesrumenbarrierfunctionandgrowthperformanceoflambsbyregulatingrumenmicrobiomeandmetabolome AT yuchaohu supplementationwithcomplexphytonutrientsenhancesrumenbarrierfunctionandgrowthperformanceoflambsbyregulatingrumenmicrobiomeandmetabolome AT nayin supplementationwithcomplexphytonutrientsenhancesrumenbarrierfunctionandgrowthperformanceoflambsbyregulatingrumenmicrobiomeandmetabolome AT xiaopingan supplementationwithcomplexphytonutrientsenhancesrumenbarrierfunctionandgrowthperformanceoflambsbyregulatingrumenmicrobiomeandmetabolome AT jingweiqi supplementationwithcomplexphytonutrientsenhancesrumenbarrierfunctionandgrowthperformanceoflambsbyregulatingrumenmicrobiomeandmetabolome AT xuanxu supplementationwithcomplexphytonutrientsenhancesrumenbarrierfunctionandgrowthperformanceoflambsbyregulatingrumenmicrobiomeandmetabolome |