Expansion of Gammadelta T Cells from Cord Blood: A Therapeutical Possibility
Gammadelta (γδ) T cells are found in both blood and tissues and have antiviral and antitumor properties. The frequency of γδ T cells in umbilical cord blood (UCB) is low, and the majority express δ1, in contrast to blood, whereas the main subset is δ2γ9 T cells. UCB γδ T cells are functionally immat...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/8529104 |
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| author | Sofia Berglund Ahmed Gaballa Piamsiri Sawaisorn Berit Sundberg Michael Uhlin |
| author_facet | Sofia Berglund Ahmed Gaballa Piamsiri Sawaisorn Berit Sundberg Michael Uhlin |
| author_sort | Sofia Berglund |
| collection | DOAJ |
| description | Gammadelta (γδ) T cells are found in both blood and tissues and have antiviral and antitumor properties. The frequency of γδ T cells in umbilical cord blood (UCB) is low, and the majority express δ1, in contrast to blood, whereas the main subset is δ2γ9 T cells. UCB γδ T cells are functionally immature, which together with their scarcity complicates the development of UCB γδ T cell therapies. We aimed to develop an effective expansion protocol for UCB γδ T cells based on zoledronate and IL-2. We found that culture with 5 μM zoledronate and 200 IU IL-2/ml medium for 14 days promoted extensive proliferation. The majority of the cultured cells were γ9δ2 T cells. The fold expansion of this, originally infrequent, subset was impressive (median and maximum fold change 253 and 1085, resp.). After culture, the cells had a polyclonal γδ T cell repertoire and the main memory subset was central memory (CD45RO+ CD27+). The cells produced cytokines such as IL-1B, IL-2, and IL-8 and displayed significant tumor-killing capacity. These results show that development of in vitro expanded UCB γδ T cell therapies is feasible. It could prove a valuable treatment modality for patients after umbilical cord blood transplantation. |
| format | Article |
| id | doaj-art-b7ca8dbf32a44a2798799389e67a22f7 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
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| series | Stem Cells International |
| spelling | doaj-art-b7ca8dbf32a44a2798799389e67a22f72025-02-03T01:22:19ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/85291048529104Expansion of Gammadelta T Cells from Cord Blood: A Therapeutical PossibilitySofia Berglund0Ahmed Gaballa1Piamsiri Sawaisorn2Berit Sundberg3Michael Uhlin4Department of Medicine, Solna, Karolinska Institutet, Stockholm, SwedenDepartment of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, SwedenDepartment of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, SwedenDepartment of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, SwedenDepartment of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, SwedenGammadelta (γδ) T cells are found in both blood and tissues and have antiviral and antitumor properties. The frequency of γδ T cells in umbilical cord blood (UCB) is low, and the majority express δ1, in contrast to blood, whereas the main subset is δ2γ9 T cells. UCB γδ T cells are functionally immature, which together with their scarcity complicates the development of UCB γδ T cell therapies. We aimed to develop an effective expansion protocol for UCB γδ T cells based on zoledronate and IL-2. We found that culture with 5 μM zoledronate and 200 IU IL-2/ml medium for 14 days promoted extensive proliferation. The majority of the cultured cells were γ9δ2 T cells. The fold expansion of this, originally infrequent, subset was impressive (median and maximum fold change 253 and 1085, resp.). After culture, the cells had a polyclonal γδ T cell repertoire and the main memory subset was central memory (CD45RO+ CD27+). The cells produced cytokines such as IL-1B, IL-2, and IL-8 and displayed significant tumor-killing capacity. These results show that development of in vitro expanded UCB γδ T cell therapies is feasible. It could prove a valuable treatment modality for patients after umbilical cord blood transplantation.http://dx.doi.org/10.1155/2018/8529104 |
| spellingShingle | Sofia Berglund Ahmed Gaballa Piamsiri Sawaisorn Berit Sundberg Michael Uhlin Expansion of Gammadelta T Cells from Cord Blood: A Therapeutical Possibility Stem Cells International |
| title | Expansion of Gammadelta T Cells from Cord Blood: A Therapeutical Possibility |
| title_full | Expansion of Gammadelta T Cells from Cord Blood: A Therapeutical Possibility |
| title_fullStr | Expansion of Gammadelta T Cells from Cord Blood: A Therapeutical Possibility |
| title_full_unstemmed | Expansion of Gammadelta T Cells from Cord Blood: A Therapeutical Possibility |
| title_short | Expansion of Gammadelta T Cells from Cord Blood: A Therapeutical Possibility |
| title_sort | expansion of gammadelta t cells from cord blood a therapeutical possibility |
| url | http://dx.doi.org/10.1155/2018/8529104 |
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