Nutritional and Microbiome Effects of a Partial Substitution of Poultry Meat with Hydrolyzed Feather Meal in Dog Diets
Two extruded diets isoenergetic, isonitrogenous, and isolipidic were formulated with poultry meal (control diet) as the source of animal-origin proteins (160 g/kg of feed) or with 90 g/kg of poultry meal and 70 g/kg of hydrolyzed feather meal (treated diet) and were fed to eight dogs (four adult fem...
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MDPI AG
2025-01-01
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| author | Fatemeh Balouei Bruno Stefanon Rosangela Armone Andrea Randazzo Biagina Chiofalo |
| author_facet | Fatemeh Balouei Bruno Stefanon Rosangela Armone Andrea Randazzo Biagina Chiofalo |
| author_sort | Fatemeh Balouei |
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| description | Two extruded diets isoenergetic, isonitrogenous, and isolipidic were formulated with poultry meal (control diet) as the source of animal-origin proteins (160 g/kg of feed) or with 90 g/kg of poultry meal and 70 g/kg of hydrolyzed feather meal (treated diet) and were fed to eight dogs (four adult female and four adult male English Setters). Body condition, muscle condition, and fecal consistency scores and body weight were monitored at the beginning of the trial and after 3, 7, 15, and 45 days, and no significant differences (<i>p</i> > 0.05) were observed between diets and between sex. Fecal samples, collected at the same time points, were analyzed for microbiota composition. No significant difference was calculated for the alpha diversity index between control diet and treated diet, nor for the diets × times of sampling interaction and for sex. Beta diversity was different (<i>p</i>-value 0.001) between the control and treated groups. The beta diversity between sexes was significantly different (<i>p</i>-value = 0.047). Linear Discriminant analysis effect size analysis revealed significant differences between dietary groups, identifying <i>Clostridiales</i>, <i>Coprococcus</i>, <i>Bacteroides plebeius</i>, <i>Eubacterium biforme</i>, <i>Catenibacterium</i>, and <i>Prevotella copri</i> as more abundant in the CTR diet, while <i>Fusobacterium, Bacteroides, Fusobacteriaceae</i>, <i>Paraprevotellaceae, Enterococcus</i>, <i>Faecalibacterium</i>, <i>Enterobacteriaceae</i>, <i>Peptostreptococcaceae</i>, and <i>Clostridium spiroforme</i> were more abundant in the treated diet. Sex differences were also significant, with 25 taxa differing between male and female dogs. Overall, the study underscores the impact of HFM and sex on gut microbiota composition in dogs, with potential implications for dietary interventions and microbiome research. |
| format | Article |
| id | doaj-art-b7b652f294be48cc83be6c3b6fa60a43 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-b7b652f294be48cc83be6c3b6fa60a432025-01-24T13:42:43ZengMDPI AGMicroorganisms2076-26072025-01-0113112110.3390/microorganisms13010121Nutritional and Microbiome Effects of a Partial Substitution of Poultry Meat with Hydrolyzed Feather Meal in Dog DietsFatemeh Balouei0Bruno Stefanon1Rosangela Armone2Andrea Randazzo3Biagina Chiofalo4Department of Agrifood, Environmental and Animal Science, University of Udine, Via delle Scienze 206, 33100 Udine, ItalyDepartment of Agrifood, Environmental and Animal Science, University of Udine, Via delle Scienze 206, 33100 Udine, ItalyDepartment of Veterinary Sciences, University of Messina, Via Palatucci Snc, 98168 Messina, ItalyDepartment of Veterinary Sciences, University of Messina, Via Palatucci Snc, 98168 Messina, ItalyDepartment of Veterinary Sciences, University of Messina, Via Palatucci Snc, 98168 Messina, ItalyTwo extruded diets isoenergetic, isonitrogenous, and isolipidic were formulated with poultry meal (control diet) as the source of animal-origin proteins (160 g/kg of feed) or with 90 g/kg of poultry meal and 70 g/kg of hydrolyzed feather meal (treated diet) and were fed to eight dogs (four adult female and four adult male English Setters). Body condition, muscle condition, and fecal consistency scores and body weight were monitored at the beginning of the trial and after 3, 7, 15, and 45 days, and no significant differences (<i>p</i> > 0.05) were observed between diets and between sex. Fecal samples, collected at the same time points, were analyzed for microbiota composition. No significant difference was calculated for the alpha diversity index between control diet and treated diet, nor for the diets × times of sampling interaction and for sex. Beta diversity was different (<i>p</i>-value 0.001) between the control and treated groups. The beta diversity between sexes was significantly different (<i>p</i>-value = 0.047). Linear Discriminant analysis effect size analysis revealed significant differences between dietary groups, identifying <i>Clostridiales</i>, <i>Coprococcus</i>, <i>Bacteroides plebeius</i>, <i>Eubacterium biforme</i>, <i>Catenibacterium</i>, and <i>Prevotella copri</i> as more abundant in the CTR diet, while <i>Fusobacterium, Bacteroides, Fusobacteriaceae</i>, <i>Paraprevotellaceae, Enterococcus</i>, <i>Faecalibacterium</i>, <i>Enterobacteriaceae</i>, <i>Peptostreptococcaceae</i>, and <i>Clostridium spiroforme</i> were more abundant in the treated diet. Sex differences were also significant, with 25 taxa differing between male and female dogs. Overall, the study underscores the impact of HFM and sex on gut microbiota composition in dogs, with potential implications for dietary interventions and microbiome research.https://www.mdpi.com/2076-2607/13/1/121hydrolyzed feather mealfecal microbiotadogsex |
| spellingShingle | Fatemeh Balouei Bruno Stefanon Rosangela Armone Andrea Randazzo Biagina Chiofalo Nutritional and Microbiome Effects of a Partial Substitution of Poultry Meat with Hydrolyzed Feather Meal in Dog Diets Microorganisms hydrolyzed feather meal fecal microbiota dog sex |
| title | Nutritional and Microbiome Effects of a Partial Substitution of Poultry Meat with Hydrolyzed Feather Meal in Dog Diets |
| title_full | Nutritional and Microbiome Effects of a Partial Substitution of Poultry Meat with Hydrolyzed Feather Meal in Dog Diets |
| title_fullStr | Nutritional and Microbiome Effects of a Partial Substitution of Poultry Meat with Hydrolyzed Feather Meal in Dog Diets |
| title_full_unstemmed | Nutritional and Microbiome Effects of a Partial Substitution of Poultry Meat with Hydrolyzed Feather Meal in Dog Diets |
| title_short | Nutritional and Microbiome Effects of a Partial Substitution of Poultry Meat with Hydrolyzed Feather Meal in Dog Diets |
| title_sort | nutritional and microbiome effects of a partial substitution of poultry meat with hydrolyzed feather meal in dog diets |
| topic | hydrolyzed feather meal fecal microbiota dog sex |
| url | https://www.mdpi.com/2076-2607/13/1/121 |
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