Comparative Analysis of Human Mesenchymal Stem Cells from Umbilical Cord, Dental Pulp, and Menstrual Blood as Sources for Cell Therapy
Although mesenchymal stem cells (MSCs) based therapy has been considered as a promising tool for tissue repair and regeneration, the optimal cell source remains unknown. Umbilical cord (UC), dental pulp (DP), and menstrual blood (MB) are easily accessible sources, which make them attractive candidat...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/3516574 |
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| author | Huaijuan Ren Yunxia Sang Fengli Zhang Zhaoqing Liu Nianmin Qi Yantian Chen |
| author_facet | Huaijuan Ren Yunxia Sang Fengli Zhang Zhaoqing Liu Nianmin Qi Yantian Chen |
| author_sort | Huaijuan Ren |
| collection | DOAJ |
| description | Although mesenchymal stem cells (MSCs) based therapy has been considered as a promising tool for tissue repair and regeneration, the optimal cell source remains unknown. Umbilical cord (UC), dental pulp (DP), and menstrual blood (MB) are easily accessible sources, which make them attractive candidates for MSCs. The goal of this study was to compare the biological characteristics, including morphology, proliferation, antiapoptosis, multilineage differentiation capacity, and immunophenotype of UC-, DP-, and MB-MSCs in order to provide a theoretical basis for clinical selection and application of these cells. As a result, all UC-, DP-, and MB-MSCs have self-renewal capacity and multipotentiality. However, the UC-MSCs seemed to have higher cell proliferation ability, while DP-MSCs may have significant advantages for osteogenic differentiation, lower cell apoptosis, and senescence. These differences may be associated with the different expression level of cytokines, including vascular endothelial growth factor, fibroblast growth factor, keratinocyte growth factor, and hepatocyte growth factor in each of the MSCs. Comprehensively, our results suggest DP-MSCs may be a desired source for clinical applications of cell therapy. |
| format | Article |
| id | doaj-art-b7b305624bde487fa2137ec378bf42fc |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-b7b305624bde487fa2137ec378bf42fc2025-02-03T06:04:58ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/35165743516574Comparative Analysis of Human Mesenchymal Stem Cells from Umbilical Cord, Dental Pulp, and Menstrual Blood as Sources for Cell TherapyHuaijuan Ren0Yunxia Sang1Fengli Zhang2Zhaoqing Liu3Nianmin Qi4Yantian Chen5Cell Culture and Bioprocess Engineering Lab, School of Pharmacy, Shanghai Jiao Tong University, Dongchuan Road 800, Minhang District, Shanghai 200240, ChinaCell Culture and Bioprocess Engineering Lab, School of Pharmacy, Shanghai Jiao Tong University, Dongchuan Road 800, Minhang District, Shanghai 200240, ChinaCell Culture and Bioprocess Engineering Lab, School of Pharmacy, Shanghai Jiao Tong University, Dongchuan Road 800, Minhang District, Shanghai 200240, ChinaAsia Stem Cell BK, Limited, Shanghai 201203, ChinaCell Culture and Bioprocess Engineering Lab, School of Pharmacy, Shanghai Jiao Tong University, Dongchuan Road 800, Minhang District, Shanghai 200240, ChinaCell Culture and Bioprocess Engineering Lab, School of Pharmacy, Shanghai Jiao Tong University, Dongchuan Road 800, Minhang District, Shanghai 200240, ChinaAlthough mesenchymal stem cells (MSCs) based therapy has been considered as a promising tool for tissue repair and regeneration, the optimal cell source remains unknown. Umbilical cord (UC), dental pulp (DP), and menstrual blood (MB) are easily accessible sources, which make them attractive candidates for MSCs. The goal of this study was to compare the biological characteristics, including morphology, proliferation, antiapoptosis, multilineage differentiation capacity, and immunophenotype of UC-, DP-, and MB-MSCs in order to provide a theoretical basis for clinical selection and application of these cells. As a result, all UC-, DP-, and MB-MSCs have self-renewal capacity and multipotentiality. However, the UC-MSCs seemed to have higher cell proliferation ability, while DP-MSCs may have significant advantages for osteogenic differentiation, lower cell apoptosis, and senescence. These differences may be associated with the different expression level of cytokines, including vascular endothelial growth factor, fibroblast growth factor, keratinocyte growth factor, and hepatocyte growth factor in each of the MSCs. Comprehensively, our results suggest DP-MSCs may be a desired source for clinical applications of cell therapy.http://dx.doi.org/10.1155/2016/3516574 |
| spellingShingle | Huaijuan Ren Yunxia Sang Fengli Zhang Zhaoqing Liu Nianmin Qi Yantian Chen Comparative Analysis of Human Mesenchymal Stem Cells from Umbilical Cord, Dental Pulp, and Menstrual Blood as Sources for Cell Therapy Stem Cells International |
| title | Comparative Analysis of Human Mesenchymal Stem Cells from Umbilical Cord, Dental Pulp, and Menstrual Blood as Sources for Cell Therapy |
| title_full | Comparative Analysis of Human Mesenchymal Stem Cells from Umbilical Cord, Dental Pulp, and Menstrual Blood as Sources for Cell Therapy |
| title_fullStr | Comparative Analysis of Human Mesenchymal Stem Cells from Umbilical Cord, Dental Pulp, and Menstrual Blood as Sources for Cell Therapy |
| title_full_unstemmed | Comparative Analysis of Human Mesenchymal Stem Cells from Umbilical Cord, Dental Pulp, and Menstrual Blood as Sources for Cell Therapy |
| title_short | Comparative Analysis of Human Mesenchymal Stem Cells from Umbilical Cord, Dental Pulp, and Menstrual Blood as Sources for Cell Therapy |
| title_sort | comparative analysis of human mesenchymal stem cells from umbilical cord dental pulp and menstrual blood as sources for cell therapy |
| url | http://dx.doi.org/10.1155/2016/3516574 |
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