Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study.
<h4>Background</h4>Chronic soil-transmitted helminth (STH) infections are associated with effects on systemic immune responses that could be caused by alterations in immune homeostasis. To investigate this, we measured the impact in children of STH infections on cytokine responses and ge...
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Public Library of Science (PLoS)
2011-06-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0001157&type=printable |
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| author | Miguel Reina Ortiz Fernanda Schreiber Susana Benitez Nely Broncano Martha E Chico Maritza Vaca Neal Alexander David J Lewis Gordon Dougan Philip J Cooper |
| author_facet | Miguel Reina Ortiz Fernanda Schreiber Susana Benitez Nely Broncano Martha E Chico Maritza Vaca Neal Alexander David J Lewis Gordon Dougan Philip J Cooper |
| author_sort | Miguel Reina Ortiz |
| collection | DOAJ |
| description | <h4>Background</h4>Chronic soil-transmitted helminth (STH) infections are associated with effects on systemic immune responses that could be caused by alterations in immune homeostasis. To investigate this, we measured the impact in children of STH infections on cytokine responses and gene expression in unstimulated blood.<h4>Methodology/principal findings</h4>Sixty children were classified as having chronic, light, or no STH infections. Peripheral blood mononuclear cells were cultured in medium for 5 days to measure cytokine accumulation. RNA was isolated from peripheral blood and gene expression analysed using microarrays. Different infection groups were compared for the purpose of analysis: STH infection (combined chronic and light vs. uninfected groups) and chronic STH infection (chronic vs. combined light and uninfected groups). The chronic STH infection effect was associated with elevated production of GM-CSF (P=0.007), IL-2 (P=0.03), IL-5 (P=0.01), and IL-10 (P=0.01). Data reduction suggested that chronic infections were primarily associated with an immune phenotype characterized by elevated IL-5 and IL-10, typical of a modified Th2-like response. Chronic STH infections were associated with the up-regulation of genes associated with immune homeostasis (IDO, P=0.03; CCL23, P=0.008, HRK, P=0.005), down-regulation of microRNA hsa-let-7d (P=0.01) and differential regulation of several genes associated with granulocyte-mediated inflammation (IL-8, down-regulated, P=0.0002; RNASE2, up-regulated, P=0.009; RNASE3, up-regulated, p=0.03).<h4>Conclusions/significance</h4>Chronic STH infections were associated with a cytokine response indicative of a modified Th2 response. There was evidence that STH infections were associated with a pattern of gene expression suggestive of the induction of homeostatic mechanisms, the differential expression of several inflammatory genes and the down-regulation of microRNA has-let-7d. Effects on immune homeostasis and the development of a modified Th2 immune response during chronic STH infections could explain the systemic immunologic effects that have been associated with these infections such as impaired immune responses to vaccines and the suppression of inflammatory diseases. |
| format | Article |
| id | doaj-art-b7b2cc848d4346f7aa8a8b861f3d8716 |
| institution | Kabale University |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2011-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-b7b2cc848d4346f7aa8a8b861f3d87162025-08-20T03:46:24ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352011-06-0156e115710.1371/journal.pntd.0001157Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study.Miguel Reina OrtizFernanda SchreiberSusana BenitezNely BroncanoMartha E ChicoMaritza VacaNeal AlexanderDavid J LewisGordon DouganPhilip J Cooper<h4>Background</h4>Chronic soil-transmitted helminth (STH) infections are associated with effects on systemic immune responses that could be caused by alterations in immune homeostasis. To investigate this, we measured the impact in children of STH infections on cytokine responses and gene expression in unstimulated blood.<h4>Methodology/principal findings</h4>Sixty children were classified as having chronic, light, or no STH infections. Peripheral blood mononuclear cells were cultured in medium for 5 days to measure cytokine accumulation. RNA was isolated from peripheral blood and gene expression analysed using microarrays. Different infection groups were compared for the purpose of analysis: STH infection (combined chronic and light vs. uninfected groups) and chronic STH infection (chronic vs. combined light and uninfected groups). The chronic STH infection effect was associated with elevated production of GM-CSF (P=0.007), IL-2 (P=0.03), IL-5 (P=0.01), and IL-10 (P=0.01). Data reduction suggested that chronic infections were primarily associated with an immune phenotype characterized by elevated IL-5 and IL-10, typical of a modified Th2-like response. Chronic STH infections were associated with the up-regulation of genes associated with immune homeostasis (IDO, P=0.03; CCL23, P=0.008, HRK, P=0.005), down-regulation of microRNA hsa-let-7d (P=0.01) and differential regulation of several genes associated with granulocyte-mediated inflammation (IL-8, down-regulated, P=0.0002; RNASE2, up-regulated, P=0.009; RNASE3, up-regulated, p=0.03).<h4>Conclusions/significance</h4>Chronic STH infections were associated with a cytokine response indicative of a modified Th2 response. There was evidence that STH infections were associated with a pattern of gene expression suggestive of the induction of homeostatic mechanisms, the differential expression of several inflammatory genes and the down-regulation of microRNA has-let-7d. Effects on immune homeostasis and the development of a modified Th2 immune response during chronic STH infections could explain the systemic immunologic effects that have been associated with these infections such as impaired immune responses to vaccines and the suppression of inflammatory diseases.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0001157&type=printable |
| spellingShingle | Miguel Reina Ortiz Fernanda Schreiber Susana Benitez Nely Broncano Martha E Chico Maritza Vaca Neal Alexander David J Lewis Gordon Dougan Philip J Cooper Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study. PLoS Neglected Tropical Diseases |
| title | Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study. |
| title_full | Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study. |
| title_fullStr | Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study. |
| title_full_unstemmed | Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study. |
| title_short | Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study. |
| title_sort | effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood a cross sectional study |
| url | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0001157&type=printable |
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