Enhancement of Fluorescent Labeling via a Composited Thin Film
Fluorescent labeling is the prevailing imaging technique in cell biological research. When statistical investigations on a large number of cells are involved, experimental study is required for both low magnification to get a reliable statistical population and high contrast to achieve accurate diag...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2014/921489 |
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| _version_ | 1832562694832521216 |
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| author | Taikei Suyama Xiaowei Ji Yaoju Zhang |
| author_facet | Taikei Suyama Xiaowei Ji Yaoju Zhang |
| author_sort | Taikei Suyama |
| collection | DOAJ |
| description | Fluorescent labeling is the prevailing imaging technique in cell biological research. When statistical investigations on a large number of cells are involved, experimental study is required for both low magnification to get a reliable statistical population and high contrast to achieve accurate diagnosis on the nature of the cells’ perturbation. As microscope objectives of low magnification generally yield low collection efficiency, such studies are limited by the fluorescence signal weakness. To overcome this technological insufficiency, Le Moal et al. proposed a method based on metal-coated substrates that enhanced the fluorescence process and improved collection efficiency in fluorescence microscope observation and that could be directly used with a common microscope setup. In this paper, we use an Ag-Si3N4-Ag multilayer film coated on the substrate and numerically analyse the optical behavior of a fluorophore which was placed above the composited film coated on the substrate. The results shows that by using an Ag-Si3N4-Ag composited film the fluorescence imaging can be enhanced remarkably. |
| format | Article |
| id | doaj-art-b7aa4fa71bf249e68f27e51e815a0203 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-b7aa4fa71bf249e68f27e51e815a02032025-02-03T01:22:03ZengWileyInternational Journal of Polymer Science1687-94221687-94302014-01-01201410.1155/2014/921489921489Enhancement of Fluorescent Labeling via a Composited Thin FilmTaikei Suyama0Xiaowei Ji1Yaoju Zhang2Department of Electrical and Computer Engineering, Akashi National College of Technology, Akashi 674-8501, JapanGraduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, JapanCollege of Physics and Electronic Information Engineering, Wenzhou University, Wenzhou 325035, ChinaFluorescent labeling is the prevailing imaging technique in cell biological research. When statistical investigations on a large number of cells are involved, experimental study is required for both low magnification to get a reliable statistical population and high contrast to achieve accurate diagnosis on the nature of the cells’ perturbation. As microscope objectives of low magnification generally yield low collection efficiency, such studies are limited by the fluorescence signal weakness. To overcome this technological insufficiency, Le Moal et al. proposed a method based on metal-coated substrates that enhanced the fluorescence process and improved collection efficiency in fluorescence microscope observation and that could be directly used with a common microscope setup. In this paper, we use an Ag-Si3N4-Ag multilayer film coated on the substrate and numerically analyse the optical behavior of a fluorophore which was placed above the composited film coated on the substrate. The results shows that by using an Ag-Si3N4-Ag composited film the fluorescence imaging can be enhanced remarkably.http://dx.doi.org/10.1155/2014/921489 |
| spellingShingle | Taikei Suyama Xiaowei Ji Yaoju Zhang Enhancement of Fluorescent Labeling via a Composited Thin Film International Journal of Polymer Science |
| title | Enhancement of Fluorescent Labeling via a Composited Thin Film |
| title_full | Enhancement of Fluorescent Labeling via a Composited Thin Film |
| title_fullStr | Enhancement of Fluorescent Labeling via a Composited Thin Film |
| title_full_unstemmed | Enhancement of Fluorescent Labeling via a Composited Thin Film |
| title_short | Enhancement of Fluorescent Labeling via a Composited Thin Film |
| title_sort | enhancement of fluorescent labeling via a composited thin film |
| url | http://dx.doi.org/10.1155/2014/921489 |
| work_keys_str_mv | AT taikeisuyama enhancementoffluorescentlabelingviaacompositedthinfilm AT xiaoweiji enhancementoffluorescentlabelingviaacompositedthinfilm AT yaojuzhang enhancementoffluorescentlabelingviaacompositedthinfilm |