Effects of Cranberry Extract (Vaccinium macrocarpon) Supplementation on Lipid Peroxidation and Inflammation in Patients with Chronic Kidney Disease (Stages 3-4): A Randomized Controlled Trial
Background. Growing evidence suggests that bioactive compounds in berry fruits may mitigate inflammation in patients with chronic kidney disease (CKD). Objectives. To evaluate cranberry (Vaccinium macrocarpon) supplementation effects on modulation of transcription factors involved in inflammation an...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-01-01
|
| Series: | Journal of Nutrition and Metabolism |
| Online Access: | http://dx.doi.org/10.1155/2024/9590066 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832553219399614464 |
|---|---|
| author | Laís de Souza Gouveia Moreira Karla Thaís Resende Teixeira Ludmila F. M. F. Cardozo Livia Alvarenga Bruna Regis Jessyca Sousa de Brito Viviane de Oliveira Leal Natalia Alvarenga Borges Isabela de Souza da Costa Brum José Carlos Carraro-Eduardo Giovanna B. Borini Andresa A. Berretta Marcelo Ribeiro-Alves Denise Mafra |
| author_facet | Laís de Souza Gouveia Moreira Karla Thaís Resende Teixeira Ludmila F. M. F. Cardozo Livia Alvarenga Bruna Regis Jessyca Sousa de Brito Viviane de Oliveira Leal Natalia Alvarenga Borges Isabela de Souza da Costa Brum José Carlos Carraro-Eduardo Giovanna B. Borini Andresa A. Berretta Marcelo Ribeiro-Alves Denise Mafra |
| author_sort | Laís de Souza Gouveia Moreira |
| collection | DOAJ |
| description | Background. Growing evidence suggests that bioactive compounds in berry fruits may mitigate inflammation in patients with chronic kidney disease (CKD). Objectives. To evaluate cranberry (Vaccinium macrocarpon) supplementation effects on modulation of transcription factors involved in inflammation and oxidative stress in nondialysis (stages 3 and 4) patients with CKD. Design/Participants. A randomized, double-blind, placebo-controlled study was performed with 30 patients to receive capsules containing cranberry extract (1000 mg/day) or placebo (1000 mg/day of corn starch) for two months. Measurements. The mRNA expression of nuclear factor-erythroid 2-related factor-2 (Nrf2) and nuclear factor-kB (NF-kB) was evaluated in peripheral blood mononuclear cells (PBMCs) by quantitative real-time polymerase chain reaction. Thiobarbituric acid reactive substances (TBARS) were measured in the plasma to assess oxidative stress. Interleukin-6 (IL-6) plasma levels were assessed by enzyme-linked immunosorbent assay and C-reactive protein (CRP) by immunoturbidimetric method. Results. Twenty-five patients completed the study: 12 in the cranberry group (56.7 ± 7.5 years and body mass index (BMI) of 29.6 ± 5.5 kg/m2) and 13 in the placebo group (58.8 ± 5.1 years and BMI 29.8 ± 5.4 kg/m2). There were no differences in NF-kB or Nrf2 mRNA expressions (p=0.99 and p=0.89) or TBARS, CRP, and IL-6 plasma levels after cranberry supplementation. Conclusions. The cranberry extract administration (1000 mg/day) did not affect Nrf2 and NF-kB mRNA expression, oxidative stress, or inflammatory markers levels in nondialysis CKD patients. This trial is registered with NCT04377919. |
| format | Article |
| id | doaj-art-b7a9b1575a4d4c21b89d6743f764ae8a |
| institution | Kabale University |
| issn | 2090-0732 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nutrition and Metabolism |
| spelling | doaj-art-b7a9b1575a4d4c21b89d6743f764ae8a2025-02-03T05:54:44ZengWileyJournal of Nutrition and Metabolism2090-07322024-01-01202410.1155/2024/9590066Effects of Cranberry Extract (Vaccinium macrocarpon) Supplementation on Lipid Peroxidation and Inflammation in Patients with Chronic Kidney Disease (Stages 3-4): A Randomized Controlled TrialLaís de Souza Gouveia Moreira0Karla Thaís Resende Teixeira1Ludmila F. M. F. Cardozo2Livia Alvarenga3Bruna Regis4Jessyca Sousa de Brito5Viviane de Oliveira Leal6Natalia Alvarenga Borges7Isabela de Souza da Costa Brum8José Carlos Carraro-Eduardo9Giovanna B. Borini10Andresa A. Berretta11Marcelo Ribeiro-Alves12Denise Mafra13Graduate Program in Medical SciencesGraduate Program in Medical SciencesGraduate Program in Nutrition SciencesGraduate Program in Nutrition SciencesGraduate Program in Cardiovascular SciencesGraduate Program in Biological Sciences -PhysiologyNutrition DivisionInstitute of NutritionGraduate Program in Medical SciencesFaculty of MedicineResearch, Development & Innovation DepartmentResearch, Development & Innovation DepartmentHIV/AIDS Clinical Research CenterGraduate Program in Medical SciencesBackground. Growing evidence suggests that bioactive compounds in berry fruits may mitigate inflammation in patients with chronic kidney disease (CKD). Objectives. To evaluate cranberry (Vaccinium macrocarpon) supplementation effects on modulation of transcription factors involved in inflammation and oxidative stress in nondialysis (stages 3 and 4) patients with CKD. Design/Participants. A randomized, double-blind, placebo-controlled study was performed with 30 patients to receive capsules containing cranberry extract (1000 mg/day) or placebo (1000 mg/day of corn starch) for two months. Measurements. The mRNA expression of nuclear factor-erythroid 2-related factor-2 (Nrf2) and nuclear factor-kB (NF-kB) was evaluated in peripheral blood mononuclear cells (PBMCs) by quantitative real-time polymerase chain reaction. Thiobarbituric acid reactive substances (TBARS) were measured in the plasma to assess oxidative stress. Interleukin-6 (IL-6) plasma levels were assessed by enzyme-linked immunosorbent assay and C-reactive protein (CRP) by immunoturbidimetric method. Results. Twenty-five patients completed the study: 12 in the cranberry group (56.7 ± 7.5 years and body mass index (BMI) of 29.6 ± 5.5 kg/m2) and 13 in the placebo group (58.8 ± 5.1 years and BMI 29.8 ± 5.4 kg/m2). There were no differences in NF-kB or Nrf2 mRNA expressions (p=0.99 and p=0.89) or TBARS, CRP, and IL-6 plasma levels after cranberry supplementation. Conclusions. The cranberry extract administration (1000 mg/day) did not affect Nrf2 and NF-kB mRNA expression, oxidative stress, or inflammatory markers levels in nondialysis CKD patients. This trial is registered with NCT04377919.http://dx.doi.org/10.1155/2024/9590066 |
| spellingShingle | Laís de Souza Gouveia Moreira Karla Thaís Resende Teixeira Ludmila F. M. F. Cardozo Livia Alvarenga Bruna Regis Jessyca Sousa de Brito Viviane de Oliveira Leal Natalia Alvarenga Borges Isabela de Souza da Costa Brum José Carlos Carraro-Eduardo Giovanna B. Borini Andresa A. Berretta Marcelo Ribeiro-Alves Denise Mafra Effects of Cranberry Extract (Vaccinium macrocarpon) Supplementation on Lipid Peroxidation and Inflammation in Patients with Chronic Kidney Disease (Stages 3-4): A Randomized Controlled Trial Journal of Nutrition and Metabolism |
| title | Effects of Cranberry Extract (Vaccinium macrocarpon) Supplementation on Lipid Peroxidation and Inflammation in Patients with Chronic Kidney Disease (Stages 3-4): A Randomized Controlled Trial |
| title_full | Effects of Cranberry Extract (Vaccinium macrocarpon) Supplementation on Lipid Peroxidation and Inflammation in Patients with Chronic Kidney Disease (Stages 3-4): A Randomized Controlled Trial |
| title_fullStr | Effects of Cranberry Extract (Vaccinium macrocarpon) Supplementation on Lipid Peroxidation and Inflammation in Patients with Chronic Kidney Disease (Stages 3-4): A Randomized Controlled Trial |
| title_full_unstemmed | Effects of Cranberry Extract (Vaccinium macrocarpon) Supplementation on Lipid Peroxidation and Inflammation in Patients with Chronic Kidney Disease (Stages 3-4): A Randomized Controlled Trial |
| title_short | Effects of Cranberry Extract (Vaccinium macrocarpon) Supplementation on Lipid Peroxidation and Inflammation in Patients with Chronic Kidney Disease (Stages 3-4): A Randomized Controlled Trial |
| title_sort | effects of cranberry extract vaccinium macrocarpon supplementation on lipid peroxidation and inflammation in patients with chronic kidney disease stages 3 4 a randomized controlled trial |
| url | http://dx.doi.org/10.1155/2024/9590066 |
| work_keys_str_mv | AT laisdesouzagouveiamoreira effectsofcranberryextractvacciniummacrocarponsupplementationonlipidperoxidationandinflammationinpatientswithchronickidneydiseasestages34arandomizedcontrolledtrial AT karlathaisresendeteixeira effectsofcranberryextractvacciniummacrocarponsupplementationonlipidperoxidationandinflammationinpatientswithchronickidneydiseasestages34arandomizedcontrolledtrial AT ludmilafmfcardozo effectsofcranberryextractvacciniummacrocarponsupplementationonlipidperoxidationandinflammationinpatientswithchronickidneydiseasestages34arandomizedcontrolledtrial AT liviaalvarenga effectsofcranberryextractvacciniummacrocarponsupplementationonlipidperoxidationandinflammationinpatientswithchronickidneydiseasestages34arandomizedcontrolledtrial AT brunaregis effectsofcranberryextractvacciniummacrocarponsupplementationonlipidperoxidationandinflammationinpatientswithchronickidneydiseasestages34arandomizedcontrolledtrial AT jessycasousadebrito effectsofcranberryextractvacciniummacrocarponsupplementationonlipidperoxidationandinflammationinpatientswithchronickidneydiseasestages34arandomizedcontrolledtrial AT vivianedeoliveiraleal effectsofcranberryextractvacciniummacrocarponsupplementationonlipidperoxidationandinflammationinpatientswithchronickidneydiseasestages34arandomizedcontrolledtrial AT nataliaalvarengaborges effectsofcranberryextractvacciniummacrocarponsupplementationonlipidperoxidationandinflammationinpatientswithchronickidneydiseasestages34arandomizedcontrolledtrial AT isabeladesouzadacostabrum effectsofcranberryextractvacciniummacrocarponsupplementationonlipidperoxidationandinflammationinpatientswithchronickidneydiseasestages34arandomizedcontrolledtrial AT josecarloscarraroeduardo effectsofcranberryextractvacciniummacrocarponsupplementationonlipidperoxidationandinflammationinpatientswithchronickidneydiseasestages34arandomizedcontrolledtrial AT giovannabborini effectsofcranberryextractvacciniummacrocarponsupplementationonlipidperoxidationandinflammationinpatientswithchronickidneydiseasestages34arandomizedcontrolledtrial AT andresaaberretta effectsofcranberryextractvacciniummacrocarponsupplementationonlipidperoxidationandinflammationinpatientswithchronickidneydiseasestages34arandomizedcontrolledtrial AT marceloribeiroalves effectsofcranberryextractvacciniummacrocarponsupplementationonlipidperoxidationandinflammationinpatientswithchronickidneydiseasestages34arandomizedcontrolledtrial AT denisemafra effectsofcranberryextractvacciniummacrocarponsupplementationonlipidperoxidationandinflammationinpatientswithchronickidneydiseasestages34arandomizedcontrolledtrial |