Prevalence of mecC Gene in MRSA at A Tertiary Hospital in Medan, Indonesia
Methicillin-resistant Staphylococcus aureus (MRSA) is resistant to β-lactam antibiotics owing to the presence of mecA and mecC resistance genes. Resistance genes in MRSA are carried by a genetic component named staphylococcal cassette chromosome mec (SCCmec). The mecC gene showed 63% similarity with...
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Journal of Pure and Applied Microbiology
2025-03-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/prevalence-of-mecc-gene-in-mrsa-at-a-tertiary-hospital-in-medan-indonesia/ |
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| author | Fiola Elsabillah Amandani Sri Amelia Nurfida Khairina Arrasyid Fera Wahyuni Ridwan Balatif |
| author_facet | Fiola Elsabillah Amandani Sri Amelia Nurfida Khairina Arrasyid Fera Wahyuni Ridwan Balatif |
| author_sort | Fiola Elsabillah Amandani |
| collection | DOAJ |
| description | Methicillin-resistant Staphylococcus aureus (MRSA) is resistant to β-lactam antibiotics owing to the presence of mecA and mecC resistance genes. Resistance genes in MRSA are carried by a genetic component named staphylococcal cassette chromosome mec (SCCmec). The mecC gene showed 63% similarity with the mecA gene. This resulted in the mecC gene not being detected by routine PCR examination, which specifically detects mecA. Data regarding the epidemiology of molecular detection of the mecC gene in Indonesia are still very limited, especially in North Sumatra Province. This study aimed to characterize MRSA resistance genes in a tertiary hospital in Medan, North Sumatra. Clinical samples of the infection were collected and identified as MRSA using the VITEK-2 compact device. A total of 80 samples from bacteremia patients in our hospital were used in this research. The detection of resistance genes is performed using conventional Polymerase Chain Reaction (PCR). Visualization of the presence of genes was performed using electrophoresis. The mec gene was detected in 79 MRSA samples (98.75%). A total of 63 samples carried two resistance genes, mecA and mecC (78.75%), 15 samples carried only mecC (18.75%), one sample carried only mecA, and only one sample carried neither mecA nor mecC. The finding of the mecC gene is a cause for concern because it cannot be detected via routine PCR. This study showed that the majority of MRSA bacteria carry a mixture of mecA and mecC genes. |
| format | Article |
| id | doaj-art-b7a41c5d2cbf42ea8eae93da3e867029 |
| institution | Kabale University |
| issn | 0973-7510 2581-690X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj-art-b7a41c5d2cbf42ea8eae93da3e8670292025-08-20T03:40:14ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2025-03-01191453458https://doi.org/10.22207/JPAM.19.1.35Prevalence of mecC Gene in MRSA at A Tertiary Hospital in Medan, IndonesiaFiola Elsabillah Amandanihttps://orcid.org/0009-0009-1348-217XSri Ameliahttps://orcid.org/0000-0001-5864-5926Nurfida Khairina Arrasyidhttps://orcid.org/0000-0001-6068-1459Fera Wahyunihttps://orcid.org/0009-0008-7083-3733Ridwan Balatifhttps://orcid.org/0000-0002-6638-3900Methicillin-resistant Staphylococcus aureus (MRSA) is resistant to β-lactam antibiotics owing to the presence of mecA and mecC resistance genes. Resistance genes in MRSA are carried by a genetic component named staphylococcal cassette chromosome mec (SCCmec). The mecC gene showed 63% similarity with the mecA gene. This resulted in the mecC gene not being detected by routine PCR examination, which specifically detects mecA. Data regarding the epidemiology of molecular detection of the mecC gene in Indonesia are still very limited, especially in North Sumatra Province. This study aimed to characterize MRSA resistance genes in a tertiary hospital in Medan, North Sumatra. Clinical samples of the infection were collected and identified as MRSA using the VITEK-2 compact device. A total of 80 samples from bacteremia patients in our hospital were used in this research. The detection of resistance genes is performed using conventional Polymerase Chain Reaction (PCR). Visualization of the presence of genes was performed using electrophoresis. The mec gene was detected in 79 MRSA samples (98.75%). A total of 63 samples carried two resistance genes, mecA and mecC (78.75%), 15 samples carried only mecC (18.75%), one sample carried only mecA, and only one sample carried neither mecA nor mecC. The finding of the mecC gene is a cause for concern because it cannot be detected via routine PCR. This study showed that the majority of MRSA bacteria carry a mixture of mecA and mecC genes.https://microbiologyjournal.org/prevalence-of-mecc-gene-in-mrsa-at-a-tertiary-hospital-in-medan-indonesia/methicillin-resistant staphylococcus aureusmolecular epidemiologypolymerase chain reactiontertiary hospital |
| spellingShingle | Fiola Elsabillah Amandani Sri Amelia Nurfida Khairina Arrasyid Fera Wahyuni Ridwan Balatif Prevalence of mecC Gene in MRSA at A Tertiary Hospital in Medan, Indonesia Journal of Pure and Applied Microbiology methicillin-resistant staphylococcus aureus molecular epidemiology polymerase chain reaction tertiary hospital |
| title | Prevalence of mecC Gene in MRSA at A Tertiary Hospital in Medan, Indonesia |
| title_full | Prevalence of mecC Gene in MRSA at A Tertiary Hospital in Medan, Indonesia |
| title_fullStr | Prevalence of mecC Gene in MRSA at A Tertiary Hospital in Medan, Indonesia |
| title_full_unstemmed | Prevalence of mecC Gene in MRSA at A Tertiary Hospital in Medan, Indonesia |
| title_short | Prevalence of mecC Gene in MRSA at A Tertiary Hospital in Medan, Indonesia |
| title_sort | prevalence of mecc gene in mrsa at a tertiary hospital in medan indonesia |
| topic | methicillin-resistant staphylococcus aureus molecular epidemiology polymerase chain reaction tertiary hospital |
| url | https://microbiologyjournal.org/prevalence-of-mecc-gene-in-mrsa-at-a-tertiary-hospital-in-medan-indonesia/ |
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