Exploring Medicinal Plants for Antimicrobial Activity and Synergistic Effects With Doxycycline Against Bacterial Species
Medicinal plants are rich sources of bioactive compounds with diverse pharmacological properties, including antimicrobial activities. This study aimed to assess the antibiofilm potential of methanol and ethanol extracts from nine selected medicinal plants, as well as their synergistic effects with d...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2024/6238852 |
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| author | Joel Frank Kenmeni Ibrahim Sifi Borel Ndezo Bisso Prudence Ngalula Kayoka-Kabongo Ulrich Joël Tsopmene Jean Paul Dzoyem |
| author_facet | Joel Frank Kenmeni Ibrahim Sifi Borel Ndezo Bisso Prudence Ngalula Kayoka-Kabongo Ulrich Joël Tsopmene Jean Paul Dzoyem |
| author_sort | Joel Frank Kenmeni |
| collection | DOAJ |
| description | Medicinal plants are rich sources of bioactive compounds with diverse pharmacological properties, including antimicrobial activities. This study aimed to assess the antibiofilm potential of methanol and ethanol extracts from nine selected medicinal plants, as well as their synergistic effects with doxycycline against Bacillus strains. Standard procedures were employed to determine the phytochemical composition, total phenolic, and flavonoid contents of the extracts. The antibacterial activity was evaluated using the broth microdilution method, while biofilm formation was assessed via the microtiter plate method. Antibiofilm activity was determined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium-bromide (MTT) assay. Combination studies were conducted using the checkerboard microdilution method. All extracts contained phenols, flavonoids, steroids, triterpenes, and anthraquinones. The methanolic extract of Psidium guajava exhibited the highest total phenolic and flavonoid contents (90.48 ± 0.55 mg GAE/g), while the ethanolic extract of Olax subscorpioidea showed the highest flavonoid content (6.48 ± 0.33 mg QE/g). Ethanol extracts of Eucalyptus globulus and Psidium guajava and methanolic extract of Syzygium jambos demonstrated significant antibacterial activity against Bacillus anthracis 34F2 Sterne strains, with a MIC value of 64 μg/mL. Biofilm formation in Bacillus strains was notably enhanced in the presence of glucose. The methanolic extract of O. subscorpioides exhibited the highest biofilm imbibition (85%), while Picralima nitida methanolic extract showed the most effective biofilm eradication (79%). The combination of Solanum torvum ethanol extract with doxycycline displayed synergistic effects against biofilm formation inhibition and eradication in all tested Bacillus strains. Taken together, Solanum torvum ethanol extract shows promise for developing new combination antibacterial therapies. |
| format | Article |
| id | doaj-art-b7a38bd810024c5e93fe8d9ee9ee80e3 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-b7a38bd810024c5e93fe8d9ee9ee80e32025-02-03T11:21:06ZengWileyThe Scientific World Journal1537-744X2024-01-01202410.1155/2024/6238852Exploring Medicinal Plants for Antimicrobial Activity and Synergistic Effects With Doxycycline Against Bacterial SpeciesJoel Frank Kenmeni0Ibrahim Sifi1Borel Ndezo Bisso2Prudence Ngalula Kayoka-Kabongo3Ulrich Joël Tsopmene4Jean Paul Dzoyem5Department of BiochemistryDepartment of BiologyDepartment of BiochemistryDepartment of Agriculture and Animal HealthDepartment of BiochemistryDepartment of BiochemistryMedicinal plants are rich sources of bioactive compounds with diverse pharmacological properties, including antimicrobial activities. This study aimed to assess the antibiofilm potential of methanol and ethanol extracts from nine selected medicinal plants, as well as their synergistic effects with doxycycline against Bacillus strains. Standard procedures were employed to determine the phytochemical composition, total phenolic, and flavonoid contents of the extracts. The antibacterial activity was evaluated using the broth microdilution method, while biofilm formation was assessed via the microtiter plate method. Antibiofilm activity was determined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium-bromide (MTT) assay. Combination studies were conducted using the checkerboard microdilution method. All extracts contained phenols, flavonoids, steroids, triterpenes, and anthraquinones. The methanolic extract of Psidium guajava exhibited the highest total phenolic and flavonoid contents (90.48 ± 0.55 mg GAE/g), while the ethanolic extract of Olax subscorpioidea showed the highest flavonoid content (6.48 ± 0.33 mg QE/g). Ethanol extracts of Eucalyptus globulus and Psidium guajava and methanolic extract of Syzygium jambos demonstrated significant antibacterial activity against Bacillus anthracis 34F2 Sterne strains, with a MIC value of 64 μg/mL. Biofilm formation in Bacillus strains was notably enhanced in the presence of glucose. The methanolic extract of O. subscorpioides exhibited the highest biofilm imbibition (85%), while Picralima nitida methanolic extract showed the most effective biofilm eradication (79%). The combination of Solanum torvum ethanol extract with doxycycline displayed synergistic effects against biofilm formation inhibition and eradication in all tested Bacillus strains. Taken together, Solanum torvum ethanol extract shows promise for developing new combination antibacterial therapies.http://dx.doi.org/10.1155/2024/6238852 |
| spellingShingle | Joel Frank Kenmeni Ibrahim Sifi Borel Ndezo Bisso Prudence Ngalula Kayoka-Kabongo Ulrich Joël Tsopmene Jean Paul Dzoyem Exploring Medicinal Plants for Antimicrobial Activity and Synergistic Effects With Doxycycline Against Bacterial Species The Scientific World Journal |
| title | Exploring Medicinal Plants for Antimicrobial Activity and Synergistic Effects With Doxycycline Against Bacterial Species |
| title_full | Exploring Medicinal Plants for Antimicrobial Activity and Synergistic Effects With Doxycycline Against Bacterial Species |
| title_fullStr | Exploring Medicinal Plants for Antimicrobial Activity and Synergistic Effects With Doxycycline Against Bacterial Species |
| title_full_unstemmed | Exploring Medicinal Plants for Antimicrobial Activity and Synergistic Effects With Doxycycline Against Bacterial Species |
| title_short | Exploring Medicinal Plants for Antimicrobial Activity and Synergistic Effects With Doxycycline Against Bacterial Species |
| title_sort | exploring medicinal plants for antimicrobial activity and synergistic effects with doxycycline against bacterial species |
| url | http://dx.doi.org/10.1155/2024/6238852 |
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