Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway
The effect of Shenfu injection on brain injury after cardiac arrest (CA) and cardiopulmonary resuscitation (CPR) along with the underlying mechanism of axonal regeneration was explored. CA/CPR model in rats was established for subsequent experiments. A total of 160 rats were randomly divided into sh...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2022/4588999 |
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| author | Haixia Deng Zhanhong Tang Peng Tuo Ruihua Wu Si Jia Xuan Zhao Deqing Huang Yuguang Gao Zhou Lan |
| author_facet | Haixia Deng Zhanhong Tang Peng Tuo Ruihua Wu Si Jia Xuan Zhao Deqing Huang Yuguang Gao Zhou Lan |
| author_sort | Haixia Deng |
| collection | DOAJ |
| description | The effect of Shenfu injection on brain injury after cardiac arrest (CA) and cardiopulmonary resuscitation (CPR) along with the underlying mechanism of axonal regeneration was explored. CA/CPR model in rats was established for subsequent experiments. A total of 160 rats were randomly divided into sham group, model group, conventional western medicine (CWM) group, Shenfu group, and antagonist group (n=32 per group). After 3 hours, 24 hours, 3 days, and 7 days of drug administration, the modified Neurological Severity Score tests were performed. The ultrastructure of the brain and hippocampus was observed by electron microscopy. Real-time quantitative polymerase chain reaction (PCR), western blotting, and immunohistochemistry were used to detect Nogo receptor (NgR) expression in the hippocampus and cerebral cortex, and Nogo–NgR expression in CA/CPR model. Neurological deficits in the model group were severe at 3 hours, 24 hours, 3 days, and 7 days after the recovery of natural circulation, whereas the neurological deficits in CWM, antagonist, and Shenfu group were relatively mild. The ultrastructure of neuronal cells in Shenfu group had relatively complete cell membranes and more vesicles than those in the model group. The results of PCR and western blotting showed lower messenger ribonucleic acid and protein expression of NgR in Shenfu group than the model group and CWM group. Immunohistochemical examination indicated a reduction of Nogo–NgR expression in Shenfu group and antagonist group. Our results suggested that Shenfu injection reduced brain injury by attenuating Nogo–NgR signaling pathway and promoting axonal regeneration. |
| format | Article |
| id | doaj-art-b79bdc8986034d3286787c6949ba01b8 |
| institution | Kabale University |
| issn | 2210-7185 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-b79bdc8986034d3286787c6949ba01b82025-02-03T06:01:49ZengWileyAnalytical Cellular Pathology2210-71852022-01-01202210.1155/2022/4588999Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR PathwayHaixia Deng0Zhanhong Tang1Peng Tuo2Ruihua Wu3Si Jia4Xuan Zhao5Deqing Huang6Yuguang Gao7Zhou Lan8Department of Surgical Intensive Care UnitDepartment of Surgical Intensive Care UnitDepartment of EmergencyGuangxi University of Chinese MedicineGuangxi University of Chinese MedicineDepartment of EmergencyDepartment of EmergencyDepartment of EmergencyDepartment of EmergencyThe effect of Shenfu injection on brain injury after cardiac arrest (CA) and cardiopulmonary resuscitation (CPR) along with the underlying mechanism of axonal regeneration was explored. CA/CPR model in rats was established for subsequent experiments. A total of 160 rats were randomly divided into sham group, model group, conventional western medicine (CWM) group, Shenfu group, and antagonist group (n=32 per group). After 3 hours, 24 hours, 3 days, and 7 days of drug administration, the modified Neurological Severity Score tests were performed. The ultrastructure of the brain and hippocampus was observed by electron microscopy. Real-time quantitative polymerase chain reaction (PCR), western blotting, and immunohistochemistry were used to detect Nogo receptor (NgR) expression in the hippocampus and cerebral cortex, and Nogo–NgR expression in CA/CPR model. Neurological deficits in the model group were severe at 3 hours, 24 hours, 3 days, and 7 days after the recovery of natural circulation, whereas the neurological deficits in CWM, antagonist, and Shenfu group were relatively mild. The ultrastructure of neuronal cells in Shenfu group had relatively complete cell membranes and more vesicles than those in the model group. The results of PCR and western blotting showed lower messenger ribonucleic acid and protein expression of NgR in Shenfu group than the model group and CWM group. Immunohistochemical examination indicated a reduction of Nogo–NgR expression in Shenfu group and antagonist group. Our results suggested that Shenfu injection reduced brain injury by attenuating Nogo–NgR signaling pathway and promoting axonal regeneration.http://dx.doi.org/10.1155/2022/4588999 |
| spellingShingle | Haixia Deng Zhanhong Tang Peng Tuo Ruihua Wu Si Jia Xuan Zhao Deqing Huang Yuguang Gao Zhou Lan Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway Analytical Cellular Pathology |
| title | Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway |
| title_full | Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway |
| title_fullStr | Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway |
| title_full_unstemmed | Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway |
| title_short | Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway |
| title_sort | shenfu injection protects brain injury in rats with cardiac arrest through nogo ngr pathway |
| url | http://dx.doi.org/10.1155/2022/4588999 |
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