Prebiotic Properties and High-Density Culture of Bifidobacterium longum B2-01, a Strain Isolated from the Intestinal Tract of Infants
In this study, a Lactobacillus strain was isolated from the feces of healthy 3-month-old infants in Guangzhou, identified and named as Bifidobacterium longum B2-01 by molecular biology, and its probiotic properties, such as in vitro antioxidant activity and hepatoprotective activity, and high-densit...
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China Food Publishing Company
2025-01-01
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| author | PENG Yunyan, ZHONG Shuying, LIU Dongmei, XU Xilin |
| author_facet | PENG Yunyan, ZHONG Shuying, LIU Dongmei, XU Xilin |
| author_sort | PENG Yunyan, ZHONG Shuying, LIU Dongmei, XU Xilin |
| collection | DOAJ |
| description | In this study, a Lactobacillus strain was isolated from the feces of healthy 3-month-old infants in Guangzhou, identified and named as Bifidobacterium longum B2-01 by molecular biology, and its probiotic properties, such as in vitro antioxidant activity and hepatoprotective activity, and high-density culture were investigated. The results showed that B2-01 had a strong ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, superoxide anion, and 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radicals and to resist lipid peroxidation. At the highest concentration of 10% (V/V) in the fermentation supernatant of DMEM medium, strain B2-01 increased the survival rates of L02 cells against hydrogen peroxide damage and alcohol damage to 89.10% and 91.38%, respectively, while significantly reducing the transaminase activity in hepatocytes. The optimal conditions for high-density culture were determined as follows: yeast extract concentration 3.45%, glucose concentration 2.48%, lactose concentration 2.79%, bacteriological peptone concentration 3%, and oligogalactose concentration 2.5%. Under these conditions, the maximum viable bacterial count of 4.20 × 109 CFU/mL was observed in the fermentation broth, which was 6.45 times as high as that before optimization (7.71 × 108 CFU/mL). |
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| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | China Food Publishing Company |
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| series | Shipin Kexue |
| spelling | doaj-art-b78b3aca6d3d44e2aba7910eb638db5a2025-02-05T09:08:12ZengChina Food Publishing CompanyShipin Kexue1002-66302025-01-01461283910.7506/spkx1002-6630-20230728-306Prebiotic Properties and High-Density Culture of Bifidobacterium longum B2-01, a Strain Isolated from the Intestinal Tract of InfantsPENG Yunyan, ZHONG Shuying, LIU Dongmei, XU Xilin0(School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China)In this study, a Lactobacillus strain was isolated from the feces of healthy 3-month-old infants in Guangzhou, identified and named as Bifidobacterium longum B2-01 by molecular biology, and its probiotic properties, such as in vitro antioxidant activity and hepatoprotective activity, and high-density culture were investigated. The results showed that B2-01 had a strong ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, superoxide anion, and 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radicals and to resist lipid peroxidation. At the highest concentration of 10% (V/V) in the fermentation supernatant of DMEM medium, strain B2-01 increased the survival rates of L02 cells against hydrogen peroxide damage and alcohol damage to 89.10% and 91.38%, respectively, while significantly reducing the transaminase activity in hepatocytes. The optimal conditions for high-density culture were determined as follows: yeast extract concentration 3.45%, glucose concentration 2.48%, lactose concentration 2.79%, bacteriological peptone concentration 3%, and oligogalactose concentration 2.5%. Under these conditions, the maximum viable bacterial count of 4.20 × 109 CFU/mL was observed in the fermentation broth, which was 6.45 times as high as that before optimization (7.71 × 108 CFU/mL).https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-1-004.pdfbifidobacterium longum; infant intestine; antioxidant activity; protection against alcoholic liver; high-density culture |
| spellingShingle | PENG Yunyan, ZHONG Shuying, LIU Dongmei, XU Xilin Prebiotic Properties and High-Density Culture of Bifidobacterium longum B2-01, a Strain Isolated from the Intestinal Tract of Infants Shipin Kexue bifidobacterium longum; infant intestine; antioxidant activity; protection against alcoholic liver; high-density culture |
| title | Prebiotic Properties and High-Density Culture of Bifidobacterium longum B2-01, a Strain Isolated from the Intestinal Tract of Infants |
| title_full | Prebiotic Properties and High-Density Culture of Bifidobacterium longum B2-01, a Strain Isolated from the Intestinal Tract of Infants |
| title_fullStr | Prebiotic Properties and High-Density Culture of Bifidobacterium longum B2-01, a Strain Isolated from the Intestinal Tract of Infants |
| title_full_unstemmed | Prebiotic Properties and High-Density Culture of Bifidobacterium longum B2-01, a Strain Isolated from the Intestinal Tract of Infants |
| title_short | Prebiotic Properties and High-Density Culture of Bifidobacterium longum B2-01, a Strain Isolated from the Intestinal Tract of Infants |
| title_sort | prebiotic properties and high density culture of bifidobacterium longum b2 01 a strain isolated from the intestinal tract of infants |
| topic | bifidobacterium longum; infant intestine; antioxidant activity; protection against alcoholic liver; high-density culture |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-1-004.pdf |
| work_keys_str_mv | AT pengyunyanzhongshuyingliudongmeixuxilin prebioticpropertiesandhighdensitycultureofbifidobacteriumlongumb201astrainisolatedfromtheintestinaltractofinfants |