Oxidative Stress and Cytoskeletal Reorganization in Hypertensive Erythrocytes
Oxidative stress is widely recognized as a key mechanism in the development of hypertension. Under pathological conditions, such as in hypertension, oxidative stress leads to irreversible posttranslational modifications of proteins, which result in loss of protein function and cellular damage. We ha...
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MDPI AG
2024-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/14/1/5 |
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| author | Ivette Martínez-Vieyra Isaac Hernández-Rojo Víctor Hugo Rosales-García Aracely Evangelina Chávez-Piña Doris Cerecedo |
| author_facet | Ivette Martínez-Vieyra Isaac Hernández-Rojo Víctor Hugo Rosales-García Aracely Evangelina Chávez-Piña Doris Cerecedo |
| author_sort | Ivette Martínez-Vieyra |
| collection | DOAJ |
| description | Oxidative stress is widely recognized as a key mechanism in the development of hypertension. Under pathological conditions, such as in hypertension, oxidative stress leads to irreversible posttranslational modifications of proteins, which result in loss of protein function and cellular damage. We have previously documented physiological and morphological changes across various blood and bone marrow cell lineages, all of which exhibit elevated oxidative stress. While cytoskeletal changes in erythrocytes have been well characterized in hereditary diseases, this is the first study, to our knowledge, to investigate cytoskeletal reorganization in erythrocytes from hypertensive patients. To this end, we compared the expression patterns and subcellular distribution of key cytoskeletal proteins in erythrocytes from hypertensive individuals with those from normotensive subjects using Western blot, flow cytometry, and confocal microscopy. Our results revealed the presence of three erythrocyte subpopulations with differential expression of glycophorin A. The persistent oxidative environment in hypertensive patients causes dysregulation in the expression of glycophorin A, Band 3 protein, protein 4.1, and ankyrin, as well as the reorganization of spectrin. These alterations in protein expression and distribution suggest that oxidative stress in hypertensive individuals may induce structural modifications, ultimately impairing erythrocyte membrane elasticity and function. |
| format | Article |
| id | doaj-art-b78a93b9520d44c8b7582c9680dd0310 |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-b78a93b9520d44c8b7582c9680dd03102025-01-24T13:19:04ZengMDPI AGAntioxidants2076-39212024-12-01141510.3390/antiox14010005Oxidative Stress and Cytoskeletal Reorganization in Hypertensive ErythrocytesIvette Martínez-Vieyra0Isaac Hernández-Rojo1Víctor Hugo Rosales-García2Aracely Evangelina Chávez-Piña3Doris Cerecedo4Laboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Mexico City 07700, MexicoLaboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Mexico City 07700, MexicoLaboratorios Centrales, Centro de Investigación y de Estudios Avanzados del IPN, Mexico City 07360, MexicoLaboratorio de Farmacología, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Mexico City 07700, MexicoLaboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Mexico City 07700, MexicoOxidative stress is widely recognized as a key mechanism in the development of hypertension. Under pathological conditions, such as in hypertension, oxidative stress leads to irreversible posttranslational modifications of proteins, which result in loss of protein function and cellular damage. We have previously documented physiological and morphological changes across various blood and bone marrow cell lineages, all of which exhibit elevated oxidative stress. While cytoskeletal changes in erythrocytes have been well characterized in hereditary diseases, this is the first study, to our knowledge, to investigate cytoskeletal reorganization in erythrocytes from hypertensive patients. To this end, we compared the expression patterns and subcellular distribution of key cytoskeletal proteins in erythrocytes from hypertensive individuals with those from normotensive subjects using Western blot, flow cytometry, and confocal microscopy. Our results revealed the presence of three erythrocyte subpopulations with differential expression of glycophorin A. The persistent oxidative environment in hypertensive patients causes dysregulation in the expression of glycophorin A, Band 3 protein, protein 4.1, and ankyrin, as well as the reorganization of spectrin. These alterations in protein expression and distribution suggest that oxidative stress in hypertensive individuals may induce structural modifications, ultimately impairing erythrocyte membrane elasticity and function.https://www.mdpi.com/2076-3921/14/1/5red blood cellsreactive oxygen speciesprotein modificationcytoskeleton reorganizationerythrocytes heterogeneity |
| spellingShingle | Ivette Martínez-Vieyra Isaac Hernández-Rojo Víctor Hugo Rosales-García Aracely Evangelina Chávez-Piña Doris Cerecedo Oxidative Stress and Cytoskeletal Reorganization in Hypertensive Erythrocytes Antioxidants red blood cells reactive oxygen species protein modification cytoskeleton reorganization erythrocytes heterogeneity |
| title | Oxidative Stress and Cytoskeletal Reorganization in Hypertensive Erythrocytes |
| title_full | Oxidative Stress and Cytoskeletal Reorganization in Hypertensive Erythrocytes |
| title_fullStr | Oxidative Stress and Cytoskeletal Reorganization in Hypertensive Erythrocytes |
| title_full_unstemmed | Oxidative Stress and Cytoskeletal Reorganization in Hypertensive Erythrocytes |
| title_short | Oxidative Stress and Cytoskeletal Reorganization in Hypertensive Erythrocytes |
| title_sort | oxidative stress and cytoskeletal reorganization in hypertensive erythrocytes |
| topic | red blood cells reactive oxygen species protein modification cytoskeleton reorganization erythrocytes heterogeneity |
| url | https://www.mdpi.com/2076-3921/14/1/5 |
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