Effects of Air Injection on the Metabolic Activity of Emulsifier-Producing Bacteria from Oil Reservoirs
Oil emulsification is one of the major mechanisms for microbially enhanced oil recovery (MEOR). Although air injection is generally recommended for field trials of MEOR in China, its influence on the microbial community structure in oil reservoirs remains poorly understood, especially activation of...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Geofluids |
| Online Access: | http://dx.doi.org/10.1155/2020/8987258 |
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| author | Yi Gu Zhenzhen Wu Guan Wang Bo Zhi Jiliang Yu Huiqiang Fan Suzhen Guo Ting Ma Guoqiang Li |
| author_facet | Yi Gu Zhenzhen Wu Guan Wang Bo Zhi Jiliang Yu Huiqiang Fan Suzhen Guo Ting Ma Guoqiang Li |
| author_sort | Yi Gu |
| collection | DOAJ |
| description | Oil emulsification is one of the major mechanisms for microbially enhanced oil recovery (MEOR). Although air injection is generally recommended for field trials of MEOR in China, its influence on the microbial community structure in oil reservoirs remains poorly understood, especially activation of emulsifier-producing bacteria. Herein, the effects of air injection on oil emulsification, nutrient consumption, oil properties, and microbial community structures were compared for activated cultures under four different oxygen content conditions: anaerobic, facultative anaerobic, intermittent aeration, and aerobic. The results showed that crude oil in aerobic and intermittent aeration cultures was emulsified effectively when nutrients were thoroughly depleted. The particle diameter of emulsified droplets was 4.74-10.02 μm. High-throughput sequencing results showed that Bacillus and Aeribacillus were effectively activated under aerobic and intermittent aeration conditions, while Tepidimicrobium and Coprothermobacter were activated under facultative anaerobic and anaerobic conditions. Real-time quantitative PCR results showed that the initial emulsifying effect was positively correlated with the abundance of Aeribacillus pallidus. |
| format | Article |
| id | doaj-art-b74c47f1521c40d48847d27419035ee2 |
| institution | Kabale University |
| issn | 1468-8115 1468-8123 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Geofluids |
| spelling | doaj-art-b74c47f1521c40d48847d27419035ee22025-02-03T05:54:27ZengWileyGeofluids1468-81151468-81232020-01-01202010.1155/2020/89872588987258Effects of Air Injection on the Metabolic Activity of Emulsifier-Producing Bacteria from Oil ReservoirsYi Gu0Zhenzhen Wu1Guan Wang2Bo Zhi3Jiliang Yu4Huiqiang Fan5Suzhen Guo6Ting Ma7Guoqiang Li8Engineering Technology Research Institute of North China Oilfield Branch of China Petroleum and Natural Gas Co., Ltd., Renqiu 062550, ChinaSchool of Life Sciences, Nankai University, Tianjin 300071, ChinaEngineering Technology Research Institute of North China Oilfield Branch of China Petroleum and Natural Gas Co., Ltd., Renqiu 062550, ChinaSchool of Life Sciences, Nankai University, Tianjin 300071, ChinaEngineering Technology Research Institute of North China Oilfield Branch of China Petroleum and Natural Gas Co., Ltd., Renqiu 062550, ChinaSchool of Life Sciences, Nankai University, Tianjin 300071, ChinaEngineering Technology Research Institute of North China Oilfield Branch of China Petroleum and Natural Gas Co., Ltd., Renqiu 062550, ChinaSchool of Life Sciences, Nankai University, Tianjin 300071, ChinaSchool of Life Sciences, Nankai University, Tianjin 300071, ChinaOil emulsification is one of the major mechanisms for microbially enhanced oil recovery (MEOR). Although air injection is generally recommended for field trials of MEOR in China, its influence on the microbial community structure in oil reservoirs remains poorly understood, especially activation of emulsifier-producing bacteria. Herein, the effects of air injection on oil emulsification, nutrient consumption, oil properties, and microbial community structures were compared for activated cultures under four different oxygen content conditions: anaerobic, facultative anaerobic, intermittent aeration, and aerobic. The results showed that crude oil in aerobic and intermittent aeration cultures was emulsified effectively when nutrients were thoroughly depleted. The particle diameter of emulsified droplets was 4.74-10.02 μm. High-throughput sequencing results showed that Bacillus and Aeribacillus were effectively activated under aerobic and intermittent aeration conditions, while Tepidimicrobium and Coprothermobacter were activated under facultative anaerobic and anaerobic conditions. Real-time quantitative PCR results showed that the initial emulsifying effect was positively correlated with the abundance of Aeribacillus pallidus.http://dx.doi.org/10.1155/2020/8987258 |
| spellingShingle | Yi Gu Zhenzhen Wu Guan Wang Bo Zhi Jiliang Yu Huiqiang Fan Suzhen Guo Ting Ma Guoqiang Li Effects of Air Injection on the Metabolic Activity of Emulsifier-Producing Bacteria from Oil Reservoirs Geofluids |
| title | Effects of Air Injection on the Metabolic Activity of Emulsifier-Producing Bacteria from Oil Reservoirs |
| title_full | Effects of Air Injection on the Metabolic Activity of Emulsifier-Producing Bacteria from Oil Reservoirs |
| title_fullStr | Effects of Air Injection on the Metabolic Activity of Emulsifier-Producing Bacteria from Oil Reservoirs |
| title_full_unstemmed | Effects of Air Injection on the Metabolic Activity of Emulsifier-Producing Bacteria from Oil Reservoirs |
| title_short | Effects of Air Injection on the Metabolic Activity of Emulsifier-Producing Bacteria from Oil Reservoirs |
| title_sort | effects of air injection on the metabolic activity of emulsifier producing bacteria from oil reservoirs |
| url | http://dx.doi.org/10.1155/2020/8987258 |
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