Quantitative ultrasound and photoacoustic assessments of red blood cell aggregation in the human radial artery

We develop concurrent US and photoacoustic (PA) imaging to characterize structural/physiological impact of in-vivo red blood cell (RBC) aggregation. PA images at 700/800/900 nm were collected from the radial arteries of 12 participants across age groups (20 s/30 s/40 s) alongside US images (21 MHz,...

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Main Authors: Taehoon Bok, Eno Hysi, Michael C. Kolios
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Photoacoustics
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Online Access:http://www.sciencedirect.com/science/article/pii/S2213597925000345
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author Taehoon Bok
Eno Hysi
Michael C. Kolios
author_facet Taehoon Bok
Eno Hysi
Michael C. Kolios
author_sort Taehoon Bok
collection DOAJ
description We develop concurrent US and photoacoustic (PA) imaging to characterize structural/physiological impact of in-vivo red blood cell (RBC) aggregation. PA images at 700/800/900 nm were collected from the radial arteries of 12 participants across age groups (20 s/30 s/40 s) alongside US images (21 MHz, VevoLAZR). RBC aggregate size was estimated from US-derived structure-factor-size-estimation (DSFSE) and PA-derived spectral-slope (SS), along with oxygen saturation (sO2). At peak systole (PS), DSFSEPS and SSPS approximated 1 RBC and −0.1 dB/MHz, respectively, across all ages, with sO2PS values of 97.1 %, 94.7 %, and 93.0 % for each group. At end diastole (ED), DSFSEED, SSED and sO2ED values were 2.6, 3.4, and 4.7 RBCs; −0.7, −0.9, and −1.2 dB/MHz; and 98.7 %, 97.2 %, and 96.7 %, respectively. Differences between SSED and SSPS (δSS) and sO2ED and sO2PS (δsO2) increased with age, indicating aging-related increases in DSFSE and δSS, as well as decreases in sO2PS and sO2ED.
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spelling doaj-art-b73f0179d01a48e3afbca9767b9f16d62025-08-20T01:55:38ZengElsevierPhotoacoustics2213-59792025-06-014310071110.1016/j.pacs.2025.100711Quantitative ultrasound and photoacoustic assessments of red blood cell aggregation in the human radial arteryTaehoon Bok0Eno Hysi1Michael C. Kolios2Keenan Research Centre for Biomedical Science, St. Michael’s Hospital, Unity Health Toronto, Toronto, Ontario M5B 1T8, Canada; Institute for Biomedical Engineering, Science and Technology (iBEST), a partnership between Toronto Metropolitan University and St. Michael’s Hospital, Toronto, Ontario M5B 1T8, CanadaKeenan Research Centre for Biomedical Science, St. Michael’s Hospital, Unity Health Toronto, Toronto, Ontario M5B 1T8, Canada; Institute for Biomedical Engineering, Science and Technology (iBEST), a partnership between Toronto Metropolitan University and St. Michael’s Hospital, Toronto, Ontario M5B 1T8, Canada; Department of Physics, Toronto Metropolitan University, Toronto, Ontario M5B 2K3, Canada; Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5S 1A1, Canada; Corresponding authors at: Keenan Research Centre for Biomedical Science, St. Michael’s Hospital, Unity Health Toronto, Toronto, Ontario M5B 1T8, Canada.Keenan Research Centre for Biomedical Science, St. Michael’s Hospital, Unity Health Toronto, Toronto, Ontario M5B 1T8, Canada; Institute for Biomedical Engineering, Science and Technology (iBEST), a partnership between Toronto Metropolitan University and St. Michael’s Hospital, Toronto, Ontario M5B 1T8, Canada; Department of Physics, Toronto Metropolitan University, Toronto, Ontario M5B 2K3, Canada; Corresponding authors at: Keenan Research Centre for Biomedical Science, St. Michael’s Hospital, Unity Health Toronto, Toronto, Ontario M5B 1T8, Canada.We develop concurrent US and photoacoustic (PA) imaging to characterize structural/physiological impact of in-vivo red blood cell (RBC) aggregation. PA images at 700/800/900 nm were collected from the radial arteries of 12 participants across age groups (20 s/30 s/40 s) alongside US images (21 MHz, VevoLAZR). RBC aggregate size was estimated from US-derived structure-factor-size-estimation (DSFSE) and PA-derived spectral-slope (SS), along with oxygen saturation (sO2). At peak systole (PS), DSFSEPS and SSPS approximated 1 RBC and −0.1 dB/MHz, respectively, across all ages, with sO2PS values of 97.1 %, 94.7 %, and 93.0 % for each group. At end diastole (ED), DSFSEED, SSED and sO2ED values were 2.6, 3.4, and 4.7 RBCs; −0.7, −0.9, and −1.2 dB/MHz; and 98.7 %, 97.2 %, and 96.7 %, respectively. Differences between SSED and SSPS (δSS) and sO2ED and sO2PS (δsO2) increased with age, indicating aging-related increases in DSFSE and δSS, as well as decreases in sO2PS and sO2ED.http://www.sciencedirect.com/science/article/pii/S2213597925000345Quantitative ultrasoundQuantitative photoacousticsRed blood cell aggregationHuman radial artery
spellingShingle Taehoon Bok
Eno Hysi
Michael C. Kolios
Quantitative ultrasound and photoacoustic assessments of red blood cell aggregation in the human radial artery
Photoacoustics
Quantitative ultrasound
Quantitative photoacoustics
Red blood cell aggregation
Human radial artery
title Quantitative ultrasound and photoacoustic assessments of red blood cell aggregation in the human radial artery
title_full Quantitative ultrasound and photoacoustic assessments of red blood cell aggregation in the human radial artery
title_fullStr Quantitative ultrasound and photoacoustic assessments of red blood cell aggregation in the human radial artery
title_full_unstemmed Quantitative ultrasound and photoacoustic assessments of red blood cell aggregation in the human radial artery
title_short Quantitative ultrasound and photoacoustic assessments of red blood cell aggregation in the human radial artery
title_sort quantitative ultrasound and photoacoustic assessments of red blood cell aggregation in the human radial artery
topic Quantitative ultrasound
Quantitative photoacoustics
Red blood cell aggregation
Human radial artery
url http://www.sciencedirect.com/science/article/pii/S2213597925000345
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