Genome-Wide Expression Profiles of Hemp (Cannabis sativa L.) in Response to Drought Stress
Drought is the main environmental factor impairing hemp growth and yield. In order to decipher the molecular responses of hemp to drought stress, transcriptome changes of drought-stressed hemp (DS1 and DS2), compared to well-watered control hemp (CK1 and CK2), were studied with RNA-Seq technology. R...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2018/3057272 |
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| author | Chunsheng Gao Chaohua Cheng Lining Zhao Yongting Yu Qing Tang Pengfei Xin Touming Liu Zhun Yan Yuan Guo Gonggu Zang |
| author_facet | Chunsheng Gao Chaohua Cheng Lining Zhao Yongting Yu Qing Tang Pengfei Xin Touming Liu Zhun Yan Yuan Guo Gonggu Zang |
| author_sort | Chunsheng Gao |
| collection | DOAJ |
| description | Drought is the main environmental factor impairing hemp growth and yield. In order to decipher the molecular responses of hemp to drought stress, transcriptome changes of drought-stressed hemp (DS1 and DS2), compared to well-watered control hemp (CK1 and CK2), were studied with RNA-Seq technology. RNA-Seq generated 9.83, 11.30, 11.66, and 11.31 M clean reads in the CK1, CK2, DS1, and DS2 libraries, respectively. A total of 1292 differentially expressed genes (DEGs), including 409 (31.66%) upregulated and 883 (68.34%) downregulated genes, were identified. The expression patterns of 12 selected genes were validated by qRT-PCR, and the results were accordant with Illumina analysis. Gene Ontology (GO) and KEGG analysis illuminated particular important biological processes and pathways, which enriched many candidate genes such as NAC, B3, peroxidase, expansin, and inositol oxygenase that may play important roles in hemp tolerance to drought. Eleven KEGG pathways were significantly influenced, the most influenced being the plant hormone signal transduction pathway with 15 differentially expressed genes. A similar expression pattern of genes involved in the abscisic acid (ABA) pathway under drought, and ABA induction, suggested that ABA is important in the drought stress response of hemp. These findings provide useful insights into the drought stress regulatory mechanism in hemp. |
| format | Article |
| id | doaj-art-b73811fb35a348a68de1722e447a0c58 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-b73811fb35a348a68de1722e447a0c582025-02-03T01:28:46ZengWileyInternational Journal of Genomics2314-436X2314-43782018-01-01201810.1155/2018/30572723057272Genome-Wide Expression Profiles of Hemp (Cannabis sativa L.) in Response to Drought StressChunsheng Gao0Chaohua Cheng1Lining Zhao2Yongting Yu3Qing Tang4Pengfei Xin5Touming Liu6Zhun Yan7Yuan Guo8Gonggu Zang9Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of the Biology and Process of Bast Fiber Crops, Ministry of Agriculture, Changsha 410205, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of the Biology and Process of Bast Fiber Crops, Ministry of Agriculture, Changsha 410205, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of the Biology and Process of Bast Fiber Crops, Ministry of Agriculture, Changsha 410205, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of the Biology and Process of Bast Fiber Crops, Ministry of Agriculture, Changsha 410205, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of the Biology and Process of Bast Fiber Crops, Ministry of Agriculture, Changsha 410205, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of the Biology and Process of Bast Fiber Crops, Ministry of Agriculture, Changsha 410205, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of the Biology and Process of Bast Fiber Crops, Ministry of Agriculture, Changsha 410205, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of the Biology and Process of Bast Fiber Crops, Ministry of Agriculture, Changsha 410205, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of the Biology and Process of Bast Fiber Crops, Ministry of Agriculture, Changsha 410205, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of the Biology and Process of Bast Fiber Crops, Ministry of Agriculture, Changsha 410205, ChinaDrought is the main environmental factor impairing hemp growth and yield. In order to decipher the molecular responses of hemp to drought stress, transcriptome changes of drought-stressed hemp (DS1 and DS2), compared to well-watered control hemp (CK1 and CK2), were studied with RNA-Seq technology. RNA-Seq generated 9.83, 11.30, 11.66, and 11.31 M clean reads in the CK1, CK2, DS1, and DS2 libraries, respectively. A total of 1292 differentially expressed genes (DEGs), including 409 (31.66%) upregulated and 883 (68.34%) downregulated genes, were identified. The expression patterns of 12 selected genes were validated by qRT-PCR, and the results were accordant with Illumina analysis. Gene Ontology (GO) and KEGG analysis illuminated particular important biological processes and pathways, which enriched many candidate genes such as NAC, B3, peroxidase, expansin, and inositol oxygenase that may play important roles in hemp tolerance to drought. Eleven KEGG pathways were significantly influenced, the most influenced being the plant hormone signal transduction pathway with 15 differentially expressed genes. A similar expression pattern of genes involved in the abscisic acid (ABA) pathway under drought, and ABA induction, suggested that ABA is important in the drought stress response of hemp. These findings provide useful insights into the drought stress regulatory mechanism in hemp.http://dx.doi.org/10.1155/2018/3057272 |
| spellingShingle | Chunsheng Gao Chaohua Cheng Lining Zhao Yongting Yu Qing Tang Pengfei Xin Touming Liu Zhun Yan Yuan Guo Gonggu Zang Genome-Wide Expression Profiles of Hemp (Cannabis sativa L.) in Response to Drought Stress International Journal of Genomics |
| title | Genome-Wide Expression Profiles of Hemp (Cannabis sativa L.) in Response to Drought Stress |
| title_full | Genome-Wide Expression Profiles of Hemp (Cannabis sativa L.) in Response to Drought Stress |
| title_fullStr | Genome-Wide Expression Profiles of Hemp (Cannabis sativa L.) in Response to Drought Stress |
| title_full_unstemmed | Genome-Wide Expression Profiles of Hemp (Cannabis sativa L.) in Response to Drought Stress |
| title_short | Genome-Wide Expression Profiles of Hemp (Cannabis sativa L.) in Response to Drought Stress |
| title_sort | genome wide expression profiles of hemp cannabis sativa l in response to drought stress |
| url | http://dx.doi.org/10.1155/2018/3057272 |
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