Validation and application of a visual LAMP assay for Mpox diagnosis — Insights from clinical samples in Thailand
Despite numerous recent advances in infectious disease diagnostic technologies including quantitative (q) polymerase chain reaction (PCR) and antigen test kit (ATK), there remains a need to develop more efficient point-of-care assays for large-scale operations. In an effort towards such a screening...
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| Language: | English |
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Elsevier
2025-12-01
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| Series: | MethodsX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016125004121 |
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| author | Wansadaj Jaroenram Yada Ongchanchai Ravee Nitiyanontakij Pawita Suwanwatthana Ratchana Ponang Wansika Kiatpathomchai |
| author_facet | Wansadaj Jaroenram Yada Ongchanchai Ravee Nitiyanontakij Pawita Suwanwatthana Ratchana Ponang Wansika Kiatpathomchai |
| author_sort | Wansadaj Jaroenram |
| collection | DOAJ |
| description | Despite numerous recent advances in infectious disease diagnostic technologies including quantitative (q) polymerase chain reaction (PCR) and antigen test kit (ATK), there remains a need to develop more efficient point-of-care assays for large-scale operations. In an effort towards such a screening test for low-resource settings, we have established a colorimetric loop-mediated isothermal amplification assay and detected the amplified DNA via naked-eye observation to maximize assay simplicity using monkeypox (Mpox) as a model. The assay is cheap, highly sensitive and specific to Mpox with the potential for translation into broader applications. • Our protocol offers a simpler, faster, and cheaper test for Mpox than the current gold-standard qPCR, with 100% accuracy. • assay enables decentralized screening and provides visually interpretable results immediately after incubation • The versatility of this protocol allows for easy adaptation to detecting new or re-emerging diseases, utilizing a visual readout for maximum simplicity. |
| format | Article |
| id | doaj-art-b7173f519c6e433c90e736b9a00fe1e1 |
| institution | Kabale University |
| issn | 2215-0161 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj-art-b7173f519c6e433c90e736b9a00fe1e12025-08-24T05:12:48ZengElsevierMethodsX2215-01612025-12-011510356810.1016/j.mex.2025.103568Validation and application of a visual LAMP assay for Mpox diagnosis — Insights from clinical samples in ThailandWansadaj Jaroenram0Yada Ongchanchai1Ravee Nitiyanontakij2Pawita Suwanwatthana3Ratchana Ponang4Wansika Kiatpathomchai5National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phahonyothin Rd., Klong Neung, Klong Luang, Pathum Thani 1212, Thailand; Corresponding author.National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phahonyothin Rd., Klong Neung, Klong Luang, Pathum Thani 1212, Thailand; Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, ThailandBamrasnaradura Infectious Diseases Institute, 126 Tivanon Rd., Talat Khwan, Mueang, Nonthaburi 11000, ThailandBamrasnaradura Infectious Diseases Institute, 126 Tivanon Rd., Talat Khwan, Mueang, Nonthaburi 11000, ThailandBamrasnaradura Infectious Diseases Institute, 126 Tivanon Rd., Talat Khwan, Mueang, Nonthaburi 11000, ThailandNational Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phahonyothin Rd., Klong Neung, Klong Luang, Pathum Thani 1212, ThailandDespite numerous recent advances in infectious disease diagnostic technologies including quantitative (q) polymerase chain reaction (PCR) and antigen test kit (ATK), there remains a need to develop more efficient point-of-care assays for large-scale operations. In an effort towards such a screening test for low-resource settings, we have established a colorimetric loop-mediated isothermal amplification assay and detected the amplified DNA via naked-eye observation to maximize assay simplicity using monkeypox (Mpox) as a model. The assay is cheap, highly sensitive and specific to Mpox with the potential for translation into broader applications. • Our protocol offers a simpler, faster, and cheaper test for Mpox than the current gold-standard qPCR, with 100% accuracy. • assay enables decentralized screening and provides visually interpretable results immediately after incubation • The versatility of this protocol allows for easy adaptation to detecting new or re-emerging diseases, utilizing a visual readout for maximum simplicity.http://www.sciencedirect.com/science/article/pii/S2215016125004121Colorimetric LAMPMonkeypox virusMpoxXylenol orangeXO |
| spellingShingle | Wansadaj Jaroenram Yada Ongchanchai Ravee Nitiyanontakij Pawita Suwanwatthana Ratchana Ponang Wansika Kiatpathomchai Validation and application of a visual LAMP assay for Mpox diagnosis — Insights from clinical samples in Thailand MethodsX Colorimetric LAMP Monkeypox virus Mpox Xylenol orange XO |
| title | Validation and application of a visual LAMP assay for Mpox diagnosis — Insights from clinical samples in Thailand |
| title_full | Validation and application of a visual LAMP assay for Mpox diagnosis — Insights from clinical samples in Thailand |
| title_fullStr | Validation and application of a visual LAMP assay for Mpox diagnosis — Insights from clinical samples in Thailand |
| title_full_unstemmed | Validation and application of a visual LAMP assay for Mpox diagnosis — Insights from clinical samples in Thailand |
| title_short | Validation and application of a visual LAMP assay for Mpox diagnosis — Insights from clinical samples in Thailand |
| title_sort | validation and application of a visual lamp assay for mpox diagnosis insights from clinical samples in thailand |
| topic | Colorimetric LAMP Monkeypox virus Mpox Xylenol orange XO |
| url | http://www.sciencedirect.com/science/article/pii/S2215016125004121 |
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