Molecular Profiling of Acute and Chronic Rejections of Renal Allografts
Both antibody mediated (AMR) and T-cell mediated (TCMR) rejections either acute or chronic represent the main reason for late graft dysfunction. In this study we aimed to evaluate differences in the intrarenal expression patterns of immune system related genes in acute and chronic rejections. Graft...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/509259 |
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| author | Hřibová Petra Honsová Eva Brabcová Irena Hrubá Petra Viklický Ondřej |
| author_facet | Hřibová Petra Honsová Eva Brabcová Irena Hrubá Petra Viklický Ondřej |
| author_sort | Hřibová Petra |
| collection | DOAJ |
| description | Both antibody mediated (AMR) and T-cell mediated (TCMR) rejections either acute or chronic represent the main reason for late graft dysfunction. In this study we aimed to evaluate differences in the intrarenal expression patterns of immune system related genes in acute and chronic rejections. Graft biopsies were performed and evaluated according to Banff classification. Using the TaqMan Low Density Array, the intrarenal expressions of 376 genes relating to immune response (B-cell activation, T-cell activation, chemokines, growth factors, immune regulators, and apoptosis) were analyzed in the four rejection categories: chronic AMR, chronic TCMR, acute AMR, and acute TCMR. The set of genes significantly upregulated in acute TCMR as compared to acute AMR was identified, while no difference in gene expressions between chronic rejections groups was found. In comparison with functioning grafts, grafts that failed within the next 24 months after the chronic rejection morphological confirmation presented at biopsy already established severe graft injury (low eGFR, higher proteinuria), longer followup, higher expression of CDC20, CXCL6, DIABLO, GABRP, KIAA0101, ME2, MMP7, NFATC4, and TGFB3 mRNA, and lower expression of CCL19 and TRADD mRNA. In conclusion, both Banff 2007 chronic rejection categories did not differ in intrarenal expression of 376 selected genes associated with immune response. |
| format | Article |
| id | doaj-art-b6fda52f230044abb6c46e2cb31a2092 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-b6fda52f230044abb6c46e2cb31a20922025-02-03T07:24:37ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/509259509259Molecular Profiling of Acute and Chronic Rejections of Renal AllograftsHřibová Petra0Honsová Eva1Brabcová Irena2Hrubá Petra3Viklický Ondřej4Transplant Laboratory, Institute for Clinical and Experimental Medicine, Vídeňská 1958/9, 14021 Prague, Czech RepublicDepartment of Transplant Pathology, Institute for Clinical and Experimental Medicine, Vídeňská 1958/9, 14021 Prague, Czech RepublicTransplant Laboratory, Institute for Clinical and Experimental Medicine, Vídeňská 1958/9, 14021 Prague, Czech RepublicTransplant Laboratory, Institute for Clinical and Experimental Medicine, Vídeňská 1958/9, 14021 Prague, Czech RepublicTransplant Laboratory, Institute for Clinical and Experimental Medicine, Vídeňská 1958/9, 14021 Prague, Czech RepublicBoth antibody mediated (AMR) and T-cell mediated (TCMR) rejections either acute or chronic represent the main reason for late graft dysfunction. In this study we aimed to evaluate differences in the intrarenal expression patterns of immune system related genes in acute and chronic rejections. Graft biopsies were performed and evaluated according to Banff classification. Using the TaqMan Low Density Array, the intrarenal expressions of 376 genes relating to immune response (B-cell activation, T-cell activation, chemokines, growth factors, immune regulators, and apoptosis) were analyzed in the four rejection categories: chronic AMR, chronic TCMR, acute AMR, and acute TCMR. The set of genes significantly upregulated in acute TCMR as compared to acute AMR was identified, while no difference in gene expressions between chronic rejections groups was found. In comparison with functioning grafts, grafts that failed within the next 24 months after the chronic rejection morphological confirmation presented at biopsy already established severe graft injury (low eGFR, higher proteinuria), longer followup, higher expression of CDC20, CXCL6, DIABLO, GABRP, KIAA0101, ME2, MMP7, NFATC4, and TGFB3 mRNA, and lower expression of CCL19 and TRADD mRNA. In conclusion, both Banff 2007 chronic rejection categories did not differ in intrarenal expression of 376 selected genes associated with immune response.http://dx.doi.org/10.1155/2013/509259 |
| spellingShingle | Hřibová Petra Honsová Eva Brabcová Irena Hrubá Petra Viklický Ondřej Molecular Profiling of Acute and Chronic Rejections of Renal Allografts Clinical and Developmental Immunology |
| title | Molecular Profiling of Acute and Chronic Rejections of Renal Allografts |
| title_full | Molecular Profiling of Acute and Chronic Rejections of Renal Allografts |
| title_fullStr | Molecular Profiling of Acute and Chronic Rejections of Renal Allografts |
| title_full_unstemmed | Molecular Profiling of Acute and Chronic Rejections of Renal Allografts |
| title_short | Molecular Profiling of Acute and Chronic Rejections of Renal Allografts |
| title_sort | molecular profiling of acute and chronic rejections of renal allografts |
| url | http://dx.doi.org/10.1155/2013/509259 |
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