AaMYB61-like and AabHLH137 jointly regulate anthocyanin biosynthesis in Actinidia arguta
Abstract Background Red Actinidia arguta has recently become highly popular because of its red appearance resulting from anthocyanin accumulation, and has gradually become an important breeding direction. However, regulators involved in anthocyanin biosynthesis have not been fully characterized in A...
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2025-01-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-025-06109-5 |
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| author | Zhe Song Yukuo Li Xu Zhan Xiaohan Li Lingshuai Ye Miaomiao Lin Ran Wang Leiming Sun Jinyong Chen Jinbao Fang Feng Wei Xiujuan Qi |
| author_facet | Zhe Song Yukuo Li Xu Zhan Xiaohan Li Lingshuai Ye Miaomiao Lin Ran Wang Leiming Sun Jinyong Chen Jinbao Fang Feng Wei Xiujuan Qi |
| author_sort | Zhe Song |
| collection | DOAJ |
| description | Abstract Background Red Actinidia arguta has recently become highly popular because of its red appearance resulting from anthocyanin accumulation, and has gradually become an important breeding direction. However, regulators involved in anthocyanin biosynthesis have not been fully characterized in A. arguta. Results Here, we demonstrated that a key R2R3-MYB transcription factor, AaMYB61-like, plays a crucial role in A. arguta anthocyanin biosynthesis. The RT-qPCR results revealed that transient overexpression of AaMYB61-like in A. arguta fruit at 90–100 DAFB significantly promoted anthocyanin biosynthesis, as did the gene expression levels of AaCHS, AaCHI, AaF3H, AaLDOX, and AaF3GT, whereas the result of VIGS revealed the opposite results in A. arguta fruit at 105–115 DAFB. A transcriptional activation assay indicated that AaMYB61-like exhibited transcriptional activation activity. Y1H and LUC assays revealed that AaMYB61-like activates the promoters of AaCHS, AaLDOX, and AaF3GT. In addition, AabHLH137 was found to be related to fruit color from the transcriptome data. We demonstrated that AaMYB61-like promotes anthocyanin biosynthesis by interacting with AabHLH137 via Y2H, BiFC, and Agrobacterium-mediated co-transformation. Conclusions Our study not only reveals the functions of AaMYB61-like and AabHLH137 in anthocyanin regulation, but also broadly enriches color regulation theory, establishing a foundation for clarifying the molecular mechanism of fruit coloration in kiwifruit. |
| format | Article |
| id | doaj-art-b6f9719ebf67455b852a62947e45a71b |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-b6f9719ebf67455b852a62947e45a71b2025-01-26T12:23:15ZengBMCBMC Plant Biology1471-22292025-01-0125111210.1186/s12870-025-06109-5AaMYB61-like and AabHLH137 jointly regulate anthocyanin biosynthesis in Actinidia argutaZhe Song0Yukuo Li1Xu Zhan2Xiaohan Li3Lingshuai Ye4Miaomiao Lin5Ran Wang6Leiming Sun7Jinyong Chen8Jinbao Fang9Feng Wei10Xiujuan Qi11School of Agricultural Sciences, Zhengzhou UniversityNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesSchool of Agricultural Sciences, Zhengzhou UniversityNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesSchool of Agricultural Sciences, Zhengzhou UniversityNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesAbstract Background Red Actinidia arguta has recently become highly popular because of its red appearance resulting from anthocyanin accumulation, and has gradually become an important breeding direction. However, regulators involved in anthocyanin biosynthesis have not been fully characterized in A. arguta. Results Here, we demonstrated that a key R2R3-MYB transcription factor, AaMYB61-like, plays a crucial role in A. arguta anthocyanin biosynthesis. The RT-qPCR results revealed that transient overexpression of AaMYB61-like in A. arguta fruit at 90–100 DAFB significantly promoted anthocyanin biosynthesis, as did the gene expression levels of AaCHS, AaCHI, AaF3H, AaLDOX, and AaF3GT, whereas the result of VIGS revealed the opposite results in A. arguta fruit at 105–115 DAFB. A transcriptional activation assay indicated that AaMYB61-like exhibited transcriptional activation activity. Y1H and LUC assays revealed that AaMYB61-like activates the promoters of AaCHS, AaLDOX, and AaF3GT. In addition, AabHLH137 was found to be related to fruit color from the transcriptome data. We demonstrated that AaMYB61-like promotes anthocyanin biosynthesis by interacting with AabHLH137 via Y2H, BiFC, and Agrobacterium-mediated co-transformation. Conclusions Our study not only reveals the functions of AaMYB61-like and AabHLH137 in anthocyanin regulation, but also broadly enriches color regulation theory, establishing a foundation for clarifying the molecular mechanism of fruit coloration in kiwifruit.https://doi.org/10.1186/s12870-025-06109-5Actinidia argutaAnthocyanin biosynthesisAaMYB61-likeAabHLH137Transcription regulation |
| spellingShingle | Zhe Song Yukuo Li Xu Zhan Xiaohan Li Lingshuai Ye Miaomiao Lin Ran Wang Leiming Sun Jinyong Chen Jinbao Fang Feng Wei Xiujuan Qi AaMYB61-like and AabHLH137 jointly regulate anthocyanin biosynthesis in Actinidia arguta BMC Plant Biology Actinidia arguta Anthocyanin biosynthesis AaMYB61-like AabHLH137 Transcription regulation |
| title | AaMYB61-like and AabHLH137 jointly regulate anthocyanin biosynthesis in Actinidia arguta |
| title_full | AaMYB61-like and AabHLH137 jointly regulate anthocyanin biosynthesis in Actinidia arguta |
| title_fullStr | AaMYB61-like and AabHLH137 jointly regulate anthocyanin biosynthesis in Actinidia arguta |
| title_full_unstemmed | AaMYB61-like and AabHLH137 jointly regulate anthocyanin biosynthesis in Actinidia arguta |
| title_short | AaMYB61-like and AabHLH137 jointly regulate anthocyanin biosynthesis in Actinidia arguta |
| title_sort | aamyb61 like and aabhlh137 jointly regulate anthocyanin biosynthesis in actinidia arguta |
| topic | Actinidia arguta Anthocyanin biosynthesis AaMYB61-like AabHLH137 Transcription regulation |
| url | https://doi.org/10.1186/s12870-025-06109-5 |
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