Karyotype Analysis and Genome Size Estimation Using Flow Cytometry of the Genus <i>Encyclia</i> Hook. (Orchidaceae: Laeliinae)
<i>Encyclia</i> Hook. is a tropical orchid known for its delightful fragrance and distinctive flower shapes, which have been widely used as superior parents for inter and intrageneric breeding. In this study, karyotype analysis shows that the number of chromosomes in diploid plants of &l...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Diversity |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-2818/17/1/57 |
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| Summary: | <i>Encyclia</i> Hook. is a tropical orchid known for its delightful fragrance and distinctive flower shapes, which have been widely used as superior parents for inter and intrageneric breeding. In this study, karyotype analysis shows that the number of chromosomes in diploid plants of <i>Encyclia</i> is 40, with all species exhibiting mesial and central filamentous chromosomes. The karyotype of diploid plants is primarily 2B. Among the three detected intergeneric hybrids, both <i>Enanthleya</i> Circus Lady ‘Coastal Star’ and <i>Guaricyclia</i> Kyoguchi ‘Fumi’ are triploid, while <i>Robertsara</i> ‘Green Wonder’ is tetraploid. By evaluating various lysates and tissues, a flow cytometry system (FCM) is developed with Galbraith’s buffer (GLB) and tender leaves. By using <i>Dendrobium officinale</i> as an external reference, the genome size of the <i>Encyclia</i> species and its intra and intergeneric hybrids are assessed, which ranges from 1.07 Gb to 3.23 Gb. These results will establish a foundation for high-throughput identification of germplasms, advance crossbreeding efforts, and enhance the understanding of the genome in <i>Encyclia</i>. |
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| ISSN: | 1424-2818 |