Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in <i>Pichia pastoris</i>
Enzymatic depolymerisation is increasingly recognised as a reliable and environmentally friendly method. The development of this technology hinges on the availability of high-quality enzymes and associated bioreaction systems for upscaling biodegradation. Microbial heterologous expression systems ha...
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| Format: | Article |
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MDPI AG
2025-01-01
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| Series: | Bioengineering |
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| author | Shuyan Wu David Hooks Gale Brightwell |
| author_facet | Shuyan Wu David Hooks Gale Brightwell |
| author_sort | Shuyan Wu |
| collection | DOAJ |
| description | Enzymatic depolymerisation is increasingly recognised as a reliable and environmentally friendly method. The development of this technology hinges on the availability of high-quality enzymes and associated bioreaction systems for upscaling biodegradation. Microbial heterologous expression systems have been studied for meeting this demand. Among these systems, the <i>Pichia pastoris</i> expression system has emerged as a widely used platform for producing secreted heterologous proteins. This article provides an overview of studies involving the recombinant expression of polymer-degrading enzymes using the <i>P. pastoris</i> expression system. Research on <i>P. pastoris</i> expression of interested enzymes with depolymerising ability, including cutinase, lipase, and laccase, are highlighted in the review. The key factors influencing the heterologous expression of polymer-degrading enzymes in <i>P. pastoris</i> are discussed, shedding light on the challenges and opportunities in the development of depolymerising biocatalysts through the <i>P. pastoris</i> expression system. |
| format | Article |
| id | doaj-art-b6d9cdc395694715878db8b37392f186 |
| institution | Kabale University |
| issn | 2306-5354 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj-art-b6d9cdc395694715878db8b37392f1862025-01-24T13:23:09ZengMDPI AGBioengineering2306-53542025-01-011216810.3390/bioengineering12010068Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in <i>Pichia pastoris</i>Shuyan Wu0David Hooks1Gale Brightwell2AgResearch Ltd., Grasslands, Palmerston North 4442, New ZealandAgResearch Ltd., Grasslands, Palmerston North 4442, New ZealandAgResearch Ltd., Grasslands, Palmerston North 4442, New ZealandEnzymatic depolymerisation is increasingly recognised as a reliable and environmentally friendly method. The development of this technology hinges on the availability of high-quality enzymes and associated bioreaction systems for upscaling biodegradation. Microbial heterologous expression systems have been studied for meeting this demand. Among these systems, the <i>Pichia pastoris</i> expression system has emerged as a widely used platform for producing secreted heterologous proteins. This article provides an overview of studies involving the recombinant expression of polymer-degrading enzymes using the <i>P. pastoris</i> expression system. Research on <i>P. pastoris</i> expression of interested enzymes with depolymerising ability, including cutinase, lipase, and laccase, are highlighted in the review. The key factors influencing the heterologous expression of polymer-degrading enzymes in <i>P. pastoris</i> are discussed, shedding light on the challenges and opportunities in the development of depolymerising biocatalysts through the <i>P. pastoris</i> expression system.https://www.mdpi.com/2306-5354/12/1/68biodegradationdepolymerisationpolymer-degrading enzymeheterologous expression<i>Pichia pastoris</i> |
| spellingShingle | Shuyan Wu David Hooks Gale Brightwell Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in <i>Pichia pastoris</i> Bioengineering biodegradation depolymerisation polymer-degrading enzyme heterologous expression <i>Pichia pastoris</i> |
| title | Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in <i>Pichia pastoris</i> |
| title_full | Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in <i>Pichia pastoris</i> |
| title_fullStr | Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in <i>Pichia pastoris</i> |
| title_full_unstemmed | Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in <i>Pichia pastoris</i> |
| title_short | Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in <i>Pichia pastoris</i> |
| title_sort | current understanding on the heterogenous expression of plastic depolymerising enzymes in i pichia pastoris i |
| topic | biodegradation depolymerisation polymer-degrading enzyme heterologous expression <i>Pichia pastoris</i> |
| url | https://www.mdpi.com/2306-5354/12/1/68 |
| work_keys_str_mv | AT shuyanwu currentunderstandingontheheterogenousexpressionofplasticdepolymerisingenzymesinipichiapastorisi AT davidhooks currentunderstandingontheheterogenousexpressionofplasticdepolymerisingenzymesinipichiapastorisi AT galebrightwell currentunderstandingontheheterogenousexpressionofplasticdepolymerisingenzymesinipichiapastorisi |