Development of an Efficient Analytical Method for the Extraction and Analysis of Biocide Contents from the Textile Test Specimens on LC-DAD
Biocides are frequently used in the manufacturing of textiles that are in direct contact with human skin. Recently regulated biocides do not have validated methods for testing; so, their presence cannot be estimated in the consumer products. Hence a rapid method was developed for the separation and...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/3047961 |
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| author | Muhammad Jahangir Uzman Khan |
| author_facet | Muhammad Jahangir Uzman Khan |
| author_sort | Muhammad Jahangir |
| collection | DOAJ |
| description | Biocides are frequently used in the manufacturing of textiles that are in direct contact with human skin. Recently regulated biocides do not have validated methods for testing; so, their presence cannot be estimated in the consumer products. Hence a rapid method was developed for the separation and quantitative analysis of biocide contents (2-methyl-4-isothaizolin (MIT), 5-chloro-2-methyl-4-isothaizolin-3-one (CIT), 2-octo-4-isothaizolin-3-one (OIT), and 5-chloro-2-(2,4-dichlorophenxy) phenol (triclosan)) from the textile test specimens. Test specimens were extracted with methanolic sonication and purified by centrifugation and filtration. Biocide contents were separated at C18 column with 0.4% acetic acid: methanol (1 : 1 v/v) under isocratic mode and detected at 280 nm wavelength. Pretreatment factors such as extraction solvent, extraction method, dilution ratio, and extraction time were optimized initially and plotted calibration curve showed regression (r2 ≥ 0.9995) in the range of 1.0–5.0 mg L−1. Recoveries were between 95% and 108% with the relative standard deviation ≤ 4%. Limits of detection (LODs) were between 0.06 mg L−1 and 0.12 mg L−1 and limits of quantification (LOQs) were between 0.21 mg L−1 and 0.38 mg L−1. From the results, conclusion was made that the method can achieve the purpose of quantitative detection and the analysis of real test specimens verified the reliability of this method. |
| format | Article |
| id | doaj-art-b6a36b19515e452ea0cf18f91e9193b3 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
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| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-b6a36b19515e452ea0cf18f91e9193b32025-02-03T05:49:52ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732020-01-01202010.1155/2020/30479613047961Development of an Efficient Analytical Method for the Extraction and Analysis of Biocide Contents from the Textile Test Specimens on LC-DADMuhammad Jahangir0Uzman Khan1Department of Chemistry, Government College University, Lahore, PakistanDepartment of Chemistry, Government College University, Lahore, PakistanBiocides are frequently used in the manufacturing of textiles that are in direct contact with human skin. Recently regulated biocides do not have validated methods for testing; so, their presence cannot be estimated in the consumer products. Hence a rapid method was developed for the separation and quantitative analysis of biocide contents (2-methyl-4-isothaizolin (MIT), 5-chloro-2-methyl-4-isothaizolin-3-one (CIT), 2-octo-4-isothaizolin-3-one (OIT), and 5-chloro-2-(2,4-dichlorophenxy) phenol (triclosan)) from the textile test specimens. Test specimens were extracted with methanolic sonication and purified by centrifugation and filtration. Biocide contents were separated at C18 column with 0.4% acetic acid: methanol (1 : 1 v/v) under isocratic mode and detected at 280 nm wavelength. Pretreatment factors such as extraction solvent, extraction method, dilution ratio, and extraction time were optimized initially and plotted calibration curve showed regression (r2 ≥ 0.9995) in the range of 1.0–5.0 mg L−1. Recoveries were between 95% and 108% with the relative standard deviation ≤ 4%. Limits of detection (LODs) were between 0.06 mg L−1 and 0.12 mg L−1 and limits of quantification (LOQs) were between 0.21 mg L−1 and 0.38 mg L−1. From the results, conclusion was made that the method can achieve the purpose of quantitative detection and the analysis of real test specimens verified the reliability of this method.http://dx.doi.org/10.1155/2020/3047961 |
| spellingShingle | Muhammad Jahangir Uzman Khan Development of an Efficient Analytical Method for the Extraction and Analysis of Biocide Contents from the Textile Test Specimens on LC-DAD Journal of Analytical Methods in Chemistry |
| title | Development of an Efficient Analytical Method for the Extraction and Analysis of Biocide Contents from the Textile Test Specimens on LC-DAD |
| title_full | Development of an Efficient Analytical Method for the Extraction and Analysis of Biocide Contents from the Textile Test Specimens on LC-DAD |
| title_fullStr | Development of an Efficient Analytical Method for the Extraction and Analysis of Biocide Contents from the Textile Test Specimens on LC-DAD |
| title_full_unstemmed | Development of an Efficient Analytical Method for the Extraction and Analysis of Biocide Contents from the Textile Test Specimens on LC-DAD |
| title_short | Development of an Efficient Analytical Method for the Extraction and Analysis of Biocide Contents from the Textile Test Specimens on LC-DAD |
| title_sort | development of an efficient analytical method for the extraction and analysis of biocide contents from the textile test specimens on lc dad |
| url | http://dx.doi.org/10.1155/2020/3047961 |
| work_keys_str_mv | AT muhammadjahangir developmentofanefficientanalyticalmethodfortheextractionandanalysisofbiocidecontentsfromthetextiletestspecimensonlcdad AT uzmankhan developmentofanefficientanalyticalmethodfortheextractionandanalysisofbiocidecontentsfromthetextiletestspecimensonlcdad |