A Novel Micellar Electrokinetic Chromatographic Method for Separation of Metal-DDTC Complexes
Micellar electrokinetic chromatography (MEKC) was examined for the separation and determination of Mo(VI), Cr(VI), Ni(II), Pd(II), and Co(III) as diethyl dithiocarbamate (DDTC) chelates. The separation was achieved from fused silica capillary (52 cm × 75 𝜇m id) with effective length 40 cm, backgroun...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/743407 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832549258186719232 |
|---|---|
| author | Arfana Mallah Saima Q. Memon Amber R. Solangi Najma Memon Kulsoom Abbassi Muhammad Yar Khuhawar |
| author_facet | Arfana Mallah Saima Q. Memon Amber R. Solangi Najma Memon Kulsoom Abbassi Muhammad Yar Khuhawar |
| author_sort | Arfana Mallah |
| collection | DOAJ |
| description | Micellar electrokinetic chromatography (MEKC) was examined for the separation and determination of Mo(VI), Cr(VI), Ni(II), Pd(II), and Co(III) as diethyl dithiocarbamate (DDTC) chelates. The separation was achieved from fused silica capillary (52 cm × 75 𝜇m id) with effective length 40 cm, background electrolyte (BGE) borate buffer pH 9.1 (25 mM), CTAB 30% (100 mM), and 1% butanol in methanol (70 : 30 : 5 v/v/v) with applied voltage of −10 kV using reverse polarity. The photodiode array detection was achieved at 225 nm. The linear calibration for each of the element was obtained within 0.16–10 𝜇g/mL with a limit of detection (LOD) 0.005–0.0167 𝜇g/mL. The separation and determination was repeatable with relative standard deviation (RSD) within 2.4–3.3% (𝑛=4) in terms of migration time and peak height/peak area. The method was applied for the determination of Mo(VI) from potatoes and almond, Ni(II) from hydrogenated vegetable oil, and Co(III) from pharmaceutical preparations with RSD within 3.9%. The results obtained were checked by standard addition and rechecked by atomic absorption spectrometry. |
| format | Article |
| id | doaj-art-b6996f6da3284b559e145a5272f87b27 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-b6996f6da3284b559e145a5272f87b272025-02-03T06:11:43ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/743407743407A Novel Micellar Electrokinetic Chromatographic Method for Separation of Metal-DDTC ComplexesArfana Mallah0Saima Q. Memon1Amber R. Solangi2Najma Memon3Kulsoom Abbassi4Muhammad Yar Khuhawar5M.A. Kazi Institute of Chemistry, University of Sindh, Jamshoro 76080, PakistanInstitute of Advanced Research Studies in Chemical Sciences, University of Sindh, Jamshoro 76080, PakistanNational Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, PakistanNational Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, PakistanM.A. Kazi Institute of Chemistry, University of Sindh, Jamshoro 76080, PakistanInstitute of Advanced Research Studies in Chemical Sciences, University of Sindh, Jamshoro 76080, PakistanMicellar electrokinetic chromatography (MEKC) was examined for the separation and determination of Mo(VI), Cr(VI), Ni(II), Pd(II), and Co(III) as diethyl dithiocarbamate (DDTC) chelates. The separation was achieved from fused silica capillary (52 cm × 75 𝜇m id) with effective length 40 cm, background electrolyte (BGE) borate buffer pH 9.1 (25 mM), CTAB 30% (100 mM), and 1% butanol in methanol (70 : 30 : 5 v/v/v) with applied voltage of −10 kV using reverse polarity. The photodiode array detection was achieved at 225 nm. The linear calibration for each of the element was obtained within 0.16–10 𝜇g/mL with a limit of detection (LOD) 0.005–0.0167 𝜇g/mL. The separation and determination was repeatable with relative standard deviation (RSD) within 2.4–3.3% (𝑛=4) in terms of migration time and peak height/peak area. The method was applied for the determination of Mo(VI) from potatoes and almond, Ni(II) from hydrogenated vegetable oil, and Co(III) from pharmaceutical preparations with RSD within 3.9%. The results obtained were checked by standard addition and rechecked by atomic absorption spectrometry.http://dx.doi.org/10.1100/2012/743407 |
| spellingShingle | Arfana Mallah Saima Q. Memon Amber R. Solangi Najma Memon Kulsoom Abbassi Muhammad Yar Khuhawar A Novel Micellar Electrokinetic Chromatographic Method for Separation of Metal-DDTC Complexes The Scientific World Journal |
| title | A Novel Micellar Electrokinetic Chromatographic Method for Separation of Metal-DDTC Complexes |
| title_full | A Novel Micellar Electrokinetic Chromatographic Method for Separation of Metal-DDTC Complexes |
| title_fullStr | A Novel Micellar Electrokinetic Chromatographic Method for Separation of Metal-DDTC Complexes |
| title_full_unstemmed | A Novel Micellar Electrokinetic Chromatographic Method for Separation of Metal-DDTC Complexes |
| title_short | A Novel Micellar Electrokinetic Chromatographic Method for Separation of Metal-DDTC Complexes |
| title_sort | novel micellar electrokinetic chromatographic method for separation of metal ddtc complexes |
| url | http://dx.doi.org/10.1100/2012/743407 |
| work_keys_str_mv | AT arfanamallah anovelmicellarelectrokineticchromatographicmethodforseparationofmetalddtccomplexes AT saimaqmemon anovelmicellarelectrokineticchromatographicmethodforseparationofmetalddtccomplexes AT amberrsolangi anovelmicellarelectrokineticchromatographicmethodforseparationofmetalddtccomplexes AT najmamemon anovelmicellarelectrokineticchromatographicmethodforseparationofmetalddtccomplexes AT kulsoomabbassi anovelmicellarelectrokineticchromatographicmethodforseparationofmetalddtccomplexes AT muhammadyarkhuhawar anovelmicellarelectrokineticchromatographicmethodforseparationofmetalddtccomplexes AT arfanamallah novelmicellarelectrokineticchromatographicmethodforseparationofmetalddtccomplexes AT saimaqmemon novelmicellarelectrokineticchromatographicmethodforseparationofmetalddtccomplexes AT amberrsolangi novelmicellarelectrokineticchromatographicmethodforseparationofmetalddtccomplexes AT najmamemon novelmicellarelectrokineticchromatographicmethodforseparationofmetalddtccomplexes AT kulsoomabbassi novelmicellarelectrokineticchromatographicmethodforseparationofmetalddtccomplexes AT muhammadyarkhuhawar novelmicellarelectrokineticchromatographicmethodforseparationofmetalddtccomplexes |