Genomic and Metabolomic Analyses of <i>Streptomyces albulus</i> with Enhanced ε-Poly-<span style="font-variant: small-caps">l</span>-lysine Production Through Adaptive Laboratory Evolution
ε-poly-<span style="font-variant: small-caps;">l</span>-lysine (ε-PL), a natural food preservative, has garnered widespread attention. It is mainly produced by <i>Streptomyces albulus</i>, but the production by wild-type strains fails to meet the demands of industri...
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2025-01-01
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| author | Xidong Ren Xinjie Sun Yan Chen Xiangheng Xi Yunzhe Ma Xinyue Jiang Xian Zhang Chenying Wang Deqiang Zhu Xinli Liu |
| author_facet | Xidong Ren Xinjie Sun Yan Chen Xiangheng Xi Yunzhe Ma Xinyue Jiang Xian Zhang Chenying Wang Deqiang Zhu Xinli Liu |
| author_sort | Xidong Ren |
| collection | DOAJ |
| description | ε-poly-<span style="font-variant: small-caps;">l</span>-lysine (ε-PL), a natural food preservative, has garnered widespread attention. It is mainly produced by <i>Streptomyces albulus</i>, but the production by wild-type strains fails to meet the demands of industrialization. To address this issue, adaptive laboratory evolution (ALE) was successfully employed in this study, subjecting <i>S. albulus</i> CICC 11022 to environmental stresses such as acidic pH and antibiotics (rifampicin, gentamicin, and streptomycin). As a result of ALE, an evolutionary strain <i>S. albulus</i> C214 was obtained, exhibiting an increase in ε-PL production and cell growth by 153.23% and 234.51%, respectively, as compared with the original strain. Genomic and metabolic analyses revealed that mutations occurred in genes responsible for transcriptional regulation, transporter, cell envelope, energy metabolism, and secondary metabolite synthesis, as well as the enrichment of metabolites involved in the biosynthesis of ε-PL. These findings hold great significance for elucidating the mechanism underlying ε-PL synthesis. |
| format | Article |
| id | doaj-art-b6630701fb5c45bbad44fa00a2534174 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-b6630701fb5c45bbad44fa00a25341742025-01-24T13:42:50ZengMDPI AGMicroorganisms2076-26072025-01-0113114910.3390/microorganisms13010149Genomic and Metabolomic Analyses of <i>Streptomyces albulus</i> with Enhanced ε-Poly-<span style="font-variant: small-caps">l</span>-lysine Production Through Adaptive Laboratory EvolutionXidong Ren0Xinjie Sun1Yan Chen2Xiangheng Xi3Yunzhe Ma4Xinyue Jiang5Xian Zhang6Chenying Wang7Deqiang Zhu8Xinli Liu9State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, ChinaKey Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Chinaε-poly-<span style="font-variant: small-caps;">l</span>-lysine (ε-PL), a natural food preservative, has garnered widespread attention. It is mainly produced by <i>Streptomyces albulus</i>, but the production by wild-type strains fails to meet the demands of industrialization. To address this issue, adaptive laboratory evolution (ALE) was successfully employed in this study, subjecting <i>S. albulus</i> CICC 11022 to environmental stresses such as acidic pH and antibiotics (rifampicin, gentamicin, and streptomycin). As a result of ALE, an evolutionary strain <i>S. albulus</i> C214 was obtained, exhibiting an increase in ε-PL production and cell growth by 153.23% and 234.51%, respectively, as compared with the original strain. Genomic and metabolic analyses revealed that mutations occurred in genes responsible for transcriptional regulation, transporter, cell envelope, energy metabolism, and secondary metabolite synthesis, as well as the enrichment of metabolites involved in the biosynthesis of ε-PL. These findings hold great significance for elucidating the mechanism underlying ε-PL synthesis.https://www.mdpi.com/2076-2607/13/1/149<i>Streptomyces albulus</i>ε-poly-<span style="font-variant: small-caps">l</span>-lysineadaptive laboratory evolutiongenomicsmetabolomics |
| spellingShingle | Xidong Ren Xinjie Sun Yan Chen Xiangheng Xi Yunzhe Ma Xinyue Jiang Xian Zhang Chenying Wang Deqiang Zhu Xinli Liu Genomic and Metabolomic Analyses of <i>Streptomyces albulus</i> with Enhanced ε-Poly-<span style="font-variant: small-caps">l</span>-lysine Production Through Adaptive Laboratory Evolution Microorganisms <i>Streptomyces albulus</i> ε-poly-<span style="font-variant: small-caps">l</span>-lysine adaptive laboratory evolution genomics metabolomics |
| title | Genomic and Metabolomic Analyses of <i>Streptomyces albulus</i> with Enhanced ε-Poly-<span style="font-variant: small-caps">l</span>-lysine Production Through Adaptive Laboratory Evolution |
| title_full | Genomic and Metabolomic Analyses of <i>Streptomyces albulus</i> with Enhanced ε-Poly-<span style="font-variant: small-caps">l</span>-lysine Production Through Adaptive Laboratory Evolution |
| title_fullStr | Genomic and Metabolomic Analyses of <i>Streptomyces albulus</i> with Enhanced ε-Poly-<span style="font-variant: small-caps">l</span>-lysine Production Through Adaptive Laboratory Evolution |
| title_full_unstemmed | Genomic and Metabolomic Analyses of <i>Streptomyces albulus</i> with Enhanced ε-Poly-<span style="font-variant: small-caps">l</span>-lysine Production Through Adaptive Laboratory Evolution |
| title_short | Genomic and Metabolomic Analyses of <i>Streptomyces albulus</i> with Enhanced ε-Poly-<span style="font-variant: small-caps">l</span>-lysine Production Through Adaptive Laboratory Evolution |
| title_sort | genomic and metabolomic analyses of i streptomyces albulus i with enhanced ε poly span style font variant small caps l span lysine production through adaptive laboratory evolution |
| topic | <i>Streptomyces albulus</i> ε-poly-<span style="font-variant: small-caps">l</span>-lysine adaptive laboratory evolution genomics metabolomics |
| url | https://www.mdpi.com/2076-2607/13/1/149 |
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