Genetic association between Class II division 1 and division 2 malocclusions with PAX9 (rs8004560) gene polymorphism in a tertiary care hospital
ABSTRACT Objective: To assess whether there is any difference in the genetic association between Class II division 1 (div. 1) and division 2 (div. 2) malocclusions using PAX9 (rs8004560) gene single nucleotide polymorphism (SNP). Material and Methods: Sixty patients from the Orthodontics departmen...
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Dental Press Editora
2024-12-01
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| Series: | Dental Press Journal of Orthodontics |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2176-94512024000600303&lng=en&tlng=en |
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| author | Monika SHUKLA Munish REDDY Kritanjali SINGH Ruchi SAINI Pradeep RAGHAV Kaynat NASSER Nupur SHARMA Aastha KAMRANI Shehla RAFIQUE Tanjula SHAIR |
| author_facet | Monika SHUKLA Munish REDDY Kritanjali SINGH Ruchi SAINI Pradeep RAGHAV Kaynat NASSER Nupur SHARMA Aastha KAMRANI Shehla RAFIQUE Tanjula SHAIR |
| author_sort | Monika SHUKLA |
| collection | DOAJ |
| description | ABSTRACT Objective: To assess whether there is any difference in the genetic association between Class II division 1 (div. 1) and division 2 (div. 2) malocclusions using PAX9 (rs8004560) gene single nucleotide polymorphism (SNP). Material and Methods: Sixty patients from the Orthodontics department of Subharti Dental College and Hospital (Meerut, India) were divided into two groups: Group 1 (Class II div. 1 malocclusion) and Group 2 (Class II div. 2 malocclusion). Then, 3 mL of blood was collected from each participant. DNA extraction was done, and Sanger Sequencing was performed from extracted DNA samples. Results: A statistically significant difference was found in the distribution of alleles among Class II div. 1 and Class II div. 2 malocclusions. The homozygous GG allele was the most prevalent among Class II div. 1 patients (76.7%), while the heterozygous AG allele was the most prevalent among Class II div. 2 patients (53.5%). Since GG was the most prevalent allele, it was used as a reference, and AA/AG were compared with GG to confirm the association. The results showed that individuals with the AG genotype seemed to be more susceptible to the development of skeletal Class II div. 2 malocclusion. Conclusion: The homozygous GG allele was the most prevalent among Class II div. 1 patients, while the heterozygous AG allele was the most prevalent among Class II div. 2 patients, suggesting that there could be a difference between the genetic association of both malocclusions. |
| format | Article |
| id | doaj-art-b64e5b78f0d44e60a26fc41ede99b013 |
| institution | OA Journals |
| issn | 2177-6709 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Dental Press Editora |
| record_format | Article |
| series | Dental Press Journal of Orthodontics |
| spelling | doaj-art-b64e5b78f0d44e60a26fc41ede99b0132025-08-20T01:57:59ZengDental Press EditoraDental Press Journal of Orthodontics2177-67092024-12-0129610.1590/2177-6709.29.6.e2424128.oarGenetic association between Class II division 1 and division 2 malocclusions with PAX9 (rs8004560) gene polymorphism in a tertiary care hospitalMonika SHUKLAhttps://orcid.org/0009-0005-2348-2123Munish REDDYhttps://orcid.org/0000-0002-3000-2571Kritanjali SINGHhttps://orcid.org/0000-0002-0533-8428Ruchi SAINIhttps://orcid.org/0000-0003-4035-8317Pradeep RAGHAVhttps://orcid.org/0000-0001-8267-1120Kaynat NASSERhttps://orcid.org/0000-0002-4105-5062Nupur SHARMAhttps://orcid.org/0009-0003-3348-0944Aastha KAMRANIhttps://orcid.org/0009-0004-2019-9026Shehla RAFIQUEhttps://orcid.org/0009-0005-7673-8524Tanjula SHAIRhttps://orcid.org/0000-0001-7894-1378ABSTRACT Objective: To assess whether there is any difference in the genetic association between Class II division 1 (div. 1) and division 2 (div. 2) malocclusions using PAX9 (rs8004560) gene single nucleotide polymorphism (SNP). Material and Methods: Sixty patients from the Orthodontics department of Subharti Dental College and Hospital (Meerut, India) were divided into two groups: Group 1 (Class II div. 1 malocclusion) and Group 2 (Class II div. 2 malocclusion). Then, 3 mL of blood was collected from each participant. DNA extraction was done, and Sanger Sequencing was performed from extracted DNA samples. Results: A statistically significant difference was found in the distribution of alleles among Class II div. 1 and Class II div. 2 malocclusions. The homozygous GG allele was the most prevalent among Class II div. 1 patients (76.7%), while the heterozygous AG allele was the most prevalent among Class II div. 2 patients (53.5%). Since GG was the most prevalent allele, it was used as a reference, and AA/AG were compared with GG to confirm the association. The results showed that individuals with the AG genotype seemed to be more susceptible to the development of skeletal Class II div. 2 malocclusion. Conclusion: The homozygous GG allele was the most prevalent among Class II div. 1 patients, while the heterozygous AG allele was the most prevalent among Class II div. 2 patients, suggesting that there could be a difference between the genetic association of both malocclusions.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2176-94512024000600303&lng=en&tlng=enMalocclusionGeneticsSNP |
| spellingShingle | Monika SHUKLA Munish REDDY Kritanjali SINGH Ruchi SAINI Pradeep RAGHAV Kaynat NASSER Nupur SHARMA Aastha KAMRANI Shehla RAFIQUE Tanjula SHAIR Genetic association between Class II division 1 and division 2 malocclusions with PAX9 (rs8004560) gene polymorphism in a tertiary care hospital Dental Press Journal of Orthodontics Malocclusion Genetics SNP |
| title | Genetic association between Class II division 1 and division 2 malocclusions with PAX9 (rs8004560) gene polymorphism in a tertiary care hospital |
| title_full | Genetic association between Class II division 1 and division 2 malocclusions with PAX9 (rs8004560) gene polymorphism in a tertiary care hospital |
| title_fullStr | Genetic association between Class II division 1 and division 2 malocclusions with PAX9 (rs8004560) gene polymorphism in a tertiary care hospital |
| title_full_unstemmed | Genetic association between Class II division 1 and division 2 malocclusions with PAX9 (rs8004560) gene polymorphism in a tertiary care hospital |
| title_short | Genetic association between Class II division 1 and division 2 malocclusions with PAX9 (rs8004560) gene polymorphism in a tertiary care hospital |
| title_sort | genetic association between class ii division 1 and division 2 malocclusions with pax9 rs8004560 gene polymorphism in a tertiary care hospital |
| topic | Malocclusion Genetics SNP |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2176-94512024000600303&lng=en&tlng=en |
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