A variant W chromosome in Centromochlus heckelii (Siluriformes, Auchenipteridae) and the role of repeated DNA in its heteromorphism
Abstract Centromochlus heckelii has the lowest diploid chromosome number (2n = 46) and the only described heteromorphic sex chromosome system in Auchenipteridae. This study presents a population of C. heckelii from the Central Amazon basin with subtle variations in the karyotype composition and a va...
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Sociedade Brasileira de Genética
2025-01-01
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| Series: | Genetics and Molecular Biology |
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| author | Chrystian Aparecido Grillo Haerter Patrik Ferreira Viana Fábio Hiroshi Takagui Sandro Tonello Vladimir Pavan Margarido Daniel Rodrigues Blanco Josiane Baccarin Traldi Roberto Laridondo Lui Eliana Feldberg |
| author_facet | Chrystian Aparecido Grillo Haerter Patrik Ferreira Viana Fábio Hiroshi Takagui Sandro Tonello Vladimir Pavan Margarido Daniel Rodrigues Blanco Josiane Baccarin Traldi Roberto Laridondo Lui Eliana Feldberg |
| author_sort | Chrystian Aparecido Grillo Haerter |
| collection | DOAJ |
| description | Abstract Centromochlus heckelii has the lowest diploid chromosome number (2n = 46) and the only described heteromorphic sex chromosome system in Auchenipteridae. This study presents a population of C. heckelii from the Central Amazon basin with subtle variations in the karyotype composition and a variant W chromosome with distinct morphology and increased C-positive heterochromatin content. In this population, the W chromosome is subtelocentric, whereas the only previous study on C. heckelii reported a metacentric W chromosome. Constitutive heterochromatin (CH) and accumulation of microsatellite motifs have significantly contributed to this W chromosome enlargement. Notably, this population exhibits numerous interstitial telomeric sites (ITSs). Some of these ITSs might represent genuine chromosomal fusion points due to the reduced 2n; however, additional mechanisms, such as chromosomal inversions, translocations, transpositions, or association with satellite DNA, are likely responsible for this unusual pattern. The 18S rDNA sites were found in both the Z and W chromosomes of all individuals. However, two individuals exhibited an additional 18S rDNA site in a single homologous of the chromosome pair 20, characterizing an intrapopulation polymorphism. The 5S rDNA sites were found in two chromosome pairs, distinguishing this population from other Centromochlinae species and further supporting it as one of the most efficient cytotaxonomic markers within the subfamily. |
| format | Article |
| id | doaj-art-b631f504049242c8abaf738128dd642e |
| institution | Kabale University |
| issn | 1678-4685 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj-art-b631f504049242c8abaf738128dd642e2025-02-04T07:38:40ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852025-01-0148110.1590/1678-4685-gmb-2024-0071A variant W chromosome in Centromochlus heckelii (Siluriformes, Auchenipteridae) and the role of repeated DNA in its heteromorphismChrystian Aparecido Grillo Haerterhttps://orcid.org/0000-0002-8772-978XPatrik Ferreira Vianahttps://orcid.org/0000-0002-8172-0725Fábio Hiroshi Takaguihttps://orcid.org/0000-0002-6963-9473Sandro Tonellohttps://orcid.org/0000-0002-7213-8717Vladimir Pavan Margaridohttps://orcid.org/0000-0002-0823-6646Daniel Rodrigues Blancohttps://orcid.org/0000-0003-1619-2417Josiane Baccarin Traldihttps://orcid.org/0000-0003-3019-7441Roberto Laridondo Luihttps://orcid.org/0000-0003-4310-4865Eliana Feldberghttps://orcid.org/0000-0002-2962-4541Abstract Centromochlus heckelii has the lowest diploid chromosome number (2n = 46) and the only described heteromorphic sex chromosome system in Auchenipteridae. This study presents a population of C. heckelii from the Central Amazon basin with subtle variations in the karyotype composition and a variant W chromosome with distinct morphology and increased C-positive heterochromatin content. In this population, the W chromosome is subtelocentric, whereas the only previous study on C. heckelii reported a metacentric W chromosome. Constitutive heterochromatin (CH) and accumulation of microsatellite motifs have significantly contributed to this W chromosome enlargement. Notably, this population exhibits numerous interstitial telomeric sites (ITSs). Some of these ITSs might represent genuine chromosomal fusion points due to the reduced 2n; however, additional mechanisms, such as chromosomal inversions, translocations, transpositions, or association with satellite DNA, are likely responsible for this unusual pattern. The 18S rDNA sites were found in both the Z and W chromosomes of all individuals. However, two individuals exhibited an additional 18S rDNA site in a single homologous of the chromosome pair 20, characterizing an intrapopulation polymorphism. The 5S rDNA sites were found in two chromosome pairs, distinguishing this population from other Centromochlinae species and further supporting it as one of the most efficient cytotaxonomic markers within the subfamily.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572025000100201&lng=en&tlng=enAuchenipteridae cytogeneticsheteromorphic sex chromosomesinterstitial telomeric sequencerepetitive DNA mapping. |
| spellingShingle | Chrystian Aparecido Grillo Haerter Patrik Ferreira Viana Fábio Hiroshi Takagui Sandro Tonello Vladimir Pavan Margarido Daniel Rodrigues Blanco Josiane Baccarin Traldi Roberto Laridondo Lui Eliana Feldberg A variant W chromosome in Centromochlus heckelii (Siluriformes, Auchenipteridae) and the role of repeated DNA in its heteromorphism Genetics and Molecular Biology Auchenipteridae cytogenetics heteromorphic sex chromosomes interstitial telomeric sequence repetitive DNA mapping. |
| title | A variant W chromosome in Centromochlus heckelii (Siluriformes, Auchenipteridae) and the role of repeated DNA in its heteromorphism |
| title_full | A variant W chromosome in Centromochlus heckelii (Siluriformes, Auchenipteridae) and the role of repeated DNA in its heteromorphism |
| title_fullStr | A variant W chromosome in Centromochlus heckelii (Siluriformes, Auchenipteridae) and the role of repeated DNA in its heteromorphism |
| title_full_unstemmed | A variant W chromosome in Centromochlus heckelii (Siluriformes, Auchenipteridae) and the role of repeated DNA in its heteromorphism |
| title_short | A variant W chromosome in Centromochlus heckelii (Siluriformes, Auchenipteridae) and the role of repeated DNA in its heteromorphism |
| title_sort | variant w chromosome in centromochlus heckelii siluriformes auchenipteridae and the role of repeated dna in its heteromorphism |
| topic | Auchenipteridae cytogenetics heteromorphic sex chromosomes interstitial telomeric sequence repetitive DNA mapping. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572025000100201&lng=en&tlng=en |
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