Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays
Phosvitin, the most highly phosphorylated metal-binding protein found in nature, binds more than 100 calcium ions, and has been identified as an agent that could be used to generate biomineralization scaffolds. Because of published reports describing phosvitin’s affinity for calcium and potential an...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2020/8831311 |
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| author | Richard D. Shipman Sean D. Doering Jack R. Hemsath Eun Joo Lee Jennifer E. Grant |
| author_facet | Richard D. Shipman Sean D. Doering Jack R. Hemsath Eun Joo Lee Jennifer E. Grant |
| author_sort | Richard D. Shipman |
| collection | DOAJ |
| description | Phosvitin, the most highly phosphorylated metal-binding protein found in nature, binds more than 100 calcium ions, and has been identified as an agent that could be used to generate biomineralization scaffolds. Because of published reports describing phosvitin’s affinity for calcium and potential antibiotic activity, this study was undertaken in order to evaluate phosvitin for both antibiotic activity against common microorganisms and the ability to protect hydroxyapatite surfaces from acid damage. To more clearly define its antibiotic action, the effects of phosvitin on Micrococcus luteus, P. mirabilis, B. cereus, E. coli, and S. epidermidis were evaluated. In both Kirby–Bauer tests and liquid culture growth inhibition assays, phosvitin inhibited M. luteus, a microorganism that thrives in the human mouth, but not the other bacteria tested. The MIC of phosvitin was determined to be 31.3 μg/mL when delivered in 1 mM CaCl2 but was 0.5 mg/mL in the absence of added calcium. Expanding on the potential impacts of phosvitin on the mouth, its action was evaluated in a model of tooth decay represented by acid-damaged hydroxyapatite discs. SEM, AFM, and FAAS analyses revealed that pretreatment of discs with phosvitin modulated the damage-induced morphology and topography changes associated with acid-damaged discs. |
| format | Article |
| id | doaj-art-b6178a4ac9cd4259aa80874e1987d005 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-b6178a4ac9cd4259aa80874e1987d0052025-02-03T05:52:25ZengWileyBiochemistry Research International2090-22472090-22552020-01-01202010.1155/2020/88313118831311Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial AssaysRichard D. Shipman0Sean D. Doering1Jack R. Hemsath2Eun Joo Lee3Jennifer E. Grant4The Applied Science Program, University of Wisconsin-Stout, Menomonie, WI 54751, USAThe Applied Science Program, University of Wisconsin-Stout, Menomonie, WI 54751, USAThe Applied Biochemistry and Molecular Biology Program, University of Wisconsin-Stout, Menomonie, WI 54751, USAFood and Nutrition Department, University of Wisconsin-Stout, Menomonie, WI 54751, USABiology Department, University of Wisconsin-Stout, Menomonie, WI 54751, USAPhosvitin, the most highly phosphorylated metal-binding protein found in nature, binds more than 100 calcium ions, and has been identified as an agent that could be used to generate biomineralization scaffolds. Because of published reports describing phosvitin’s affinity for calcium and potential antibiotic activity, this study was undertaken in order to evaluate phosvitin for both antibiotic activity against common microorganisms and the ability to protect hydroxyapatite surfaces from acid damage. To more clearly define its antibiotic action, the effects of phosvitin on Micrococcus luteus, P. mirabilis, B. cereus, E. coli, and S. epidermidis were evaluated. In both Kirby–Bauer tests and liquid culture growth inhibition assays, phosvitin inhibited M. luteus, a microorganism that thrives in the human mouth, but not the other bacteria tested. The MIC of phosvitin was determined to be 31.3 μg/mL when delivered in 1 mM CaCl2 but was 0.5 mg/mL in the absence of added calcium. Expanding on the potential impacts of phosvitin on the mouth, its action was evaluated in a model of tooth decay represented by acid-damaged hydroxyapatite discs. SEM, AFM, and FAAS analyses revealed that pretreatment of discs with phosvitin modulated the damage-induced morphology and topography changes associated with acid-damaged discs.http://dx.doi.org/10.1155/2020/8831311 |
| spellingShingle | Richard D. Shipman Sean D. Doering Jack R. Hemsath Eun Joo Lee Jennifer E. Grant Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays Biochemistry Research International |
| title | Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays |
| title_full | Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays |
| title_fullStr | Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays |
| title_full_unstemmed | Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays |
| title_short | Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays |
| title_sort | activity of phosvitin in hydroxyapatite acid damage immersion and antimicrobial assays |
| url | http://dx.doi.org/10.1155/2020/8831311 |
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