Moringa oleifera leaf extracts inhibit 6β-hydroxylation of testosterone by CYP3A4
Background: Moringa oleifera is a tropical tree often used as a herbal medicine, including by people who test positive for HIV. Since herbal constituents may interact with drugs via inhibition of metabolizing enzymes, we investigated the effects of extracts of M. oleifera on the CYP3A4-mediated 6ß-h...
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| Format: | Article |
| Language: | English |
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The Journal of Infection in Developing Countries
2008-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/201 |
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| author | Tsitsi G. Monera Alan R. Wolfe Charles C. Maponga Leslie Z. Benet Joseph Guglielmo |
| author_facet | Tsitsi G. Monera Alan R. Wolfe Charles C. Maponga Leslie Z. Benet Joseph Guglielmo |
| author_sort | Tsitsi G. Monera |
| collection | DOAJ |
| description | Background: Moringa oleifera is a tropical tree often used as a herbal medicine, including by people who test positive for HIV. Since herbal constituents may interact with drugs via inhibition of metabolizing enzymes, we investigated the effects of extracts of M. oleifera on the CYP3A4-mediated 6ß-hydroxylation of testosterone.
Methods: Methanolic and aqueous leaf and root of extracts of M. oleifera with concentrations between 0.01 and 10 mg/ml were incubated with testosterone and mixed-sex human liver microsomes in the presence of NADPH. Metabolite concentrations were determined by HPLC. The cytotoxicity of the extracts was tested with HepG2 cells using the MTT formazan assay.
Results: Significant CYP3A4 inhibitory effects were found, with IC50 values of 0.5 and 2.5 mg/ml for leaf-methanol and leaf-water extracts, respectively. Root extracts were less active. Cytotoxicity was observed only with the leaf-water extract (IC50 = 6 mg/ml).
Conclusions: Further investigation is warranted to elucidate the potential of M. oleifera for clinically significant interactions with antiretroviral and other drugs. |
| format | Article |
| id | doaj-art-b612b01ea02842ce975b51e4f7903ff1 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2008-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-b612b01ea02842ce975b51e4f7903ff12025-08-20T02:14:19ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802008-10-0120510.3855/jidc.201Moringa oleifera leaf extracts inhibit 6β-hydroxylation of testosterone by CYP3A4Tsitsi G. Monera0Alan R. Wolfe1Charles C. Maponga2Leslie Z. Benet3Joseph Guglielmo4University of Zimbabwe, School of Pharmacy, HarareUniversity of California San Francisco, Department ofClinical Pharmacy, San Francisco, CaliforniaUniversity of Zimbabwe, School of Pharmacy, HarareUniversity of California San Francisco, Department ofClinical Pharmacy, San Francisco, CaliforniaUniversity of California San Francisco, Department ofClinical Pharmacy, San Francisco, CaliforniaBackground: Moringa oleifera is a tropical tree often used as a herbal medicine, including by people who test positive for HIV. Since herbal constituents may interact with drugs via inhibition of metabolizing enzymes, we investigated the effects of extracts of M. oleifera on the CYP3A4-mediated 6ß-hydroxylation of testosterone. Methods: Methanolic and aqueous leaf and root of extracts of M. oleifera with concentrations between 0.01 and 10 mg/ml were incubated with testosterone and mixed-sex human liver microsomes in the presence of NADPH. Metabolite concentrations were determined by HPLC. The cytotoxicity of the extracts was tested with HepG2 cells using the MTT formazan assay. Results: Significant CYP3A4 inhibitory effects were found, with IC50 values of 0.5 and 2.5 mg/ml for leaf-methanol and leaf-water extracts, respectively. Root extracts were less active. Cytotoxicity was observed only with the leaf-water extract (IC50 = 6 mg/ml). Conclusions: Further investigation is warranted to elucidate the potential of M. oleifera for clinically significant interactions with antiretroviral and other drugs.https://jidc.org/index.php/journal/article/view/201Moringa oleiferaCYP3A4herb-drug interaction |
| spellingShingle | Tsitsi G. Monera Alan R. Wolfe Charles C. Maponga Leslie Z. Benet Joseph Guglielmo Moringa oleifera leaf extracts inhibit 6β-hydroxylation of testosterone by CYP3A4 Journal of Infection in Developing Countries Moringa oleifera CYP3A4 herb-drug interaction |
| title | Moringa oleifera leaf extracts inhibit 6β-hydroxylation of testosterone by CYP3A4 |
| title_full | Moringa oleifera leaf extracts inhibit 6β-hydroxylation of testosterone by CYP3A4 |
| title_fullStr | Moringa oleifera leaf extracts inhibit 6β-hydroxylation of testosterone by CYP3A4 |
| title_full_unstemmed | Moringa oleifera leaf extracts inhibit 6β-hydroxylation of testosterone by CYP3A4 |
| title_short | Moringa oleifera leaf extracts inhibit 6β-hydroxylation of testosterone by CYP3A4 |
| title_sort | moringa oleifera leaf extracts inhibit 6β hydroxylation of testosterone by cyp3a4 |
| topic | Moringa oleifera CYP3A4 herb-drug interaction |
| url | https://jidc.org/index.php/journal/article/view/201 |
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