Integrating multi-omics data to identify the role of Aggrephagy-related genes in tumor microenvironment and key tumorigenesis factors of GB from the perspective of single-cell sequencing
Abstract This study presents a pioneering exploration into the role of aggrephagy-related genes (ARGs) in glioblastoma (GB), a kind of malignant tumor which is highly invasive and resistant to a series of therapy. Utilizing single-cell sequencing to dissect their influence on the tumor microenvironm...
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| Format: | Article |
| Language: | English |
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Springer
2025-05-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-025-02431-4 |
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| author | Zipei Chen Shengke Zhang Chenglu Jiang Lai Jiang Haiqing Chen Jinbang Huang Jie Liu Guanhu Yang Xiufang Luo Hao Chi Jiangping Fu |
| author_facet | Zipei Chen Shengke Zhang Chenglu Jiang Lai Jiang Haiqing Chen Jinbang Huang Jie Liu Guanhu Yang Xiufang Luo Hao Chi Jiangping Fu |
| author_sort | Zipei Chen |
| collection | DOAJ |
| description | Abstract This study presents a pioneering exploration into the role of aggrephagy-related genes (ARGs) in glioblastoma (GB), a kind of malignant tumor which is highly invasive and resistant to a series of therapy. Utilizing single-cell sequencing to dissect their influence on the tumor microenvironment (TME) and tumorigenesis. By applying non-negative matrix factorization for dimensionality reduction and clustering of single-cell data, distinct cellular subtypes within the TME influenced by ARGs were identified, uncovering their functions and interactions. The investigation extends to validating the prognostic significance of ARGs and their potential in predicting immunotherapy outcomes. Molecular docking analysis of key ARGs further highlights TUBA1C and UBB as promising therapeutic targets, offering novel insights into GB’s complex biology and suggesting a targeted approach for therapy, which is characterized by some crucial pathways in our analysis, including PI3k-akt and TGF-beta pathways. This comprehensive single-cell level examination not only advances our understanding of aggrephagy’s role in GB but also proposes new avenues for prognosis and treatment strategies, emphasizing the critical impact of ARGs on the TME and GB progression. |
| format | Article |
| id | doaj-art-b60d28e8e3ce4a1d8e9b2536f51a2cad |
| institution | DOAJ |
| issn | 2730-6011 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-b60d28e8e3ce4a1d8e9b2536f51a2cad2025-08-20T03:10:32ZengSpringerDiscover Oncology2730-60112025-05-0116112710.1007/s12672-025-02431-4Integrating multi-omics data to identify the role of Aggrephagy-related genes in tumor microenvironment and key tumorigenesis factors of GB from the perspective of single-cell sequencingZipei Chen0Shengke Zhang1Chenglu Jiang2Lai Jiang3Haiqing Chen4Jinbang Huang5Jie Liu6Guanhu Yang7Xiufang Luo8Hao Chi9Jiangping Fu10Department of Oncology, Dazhou Central HospitalDepartment of Clinical, Clinical Medical College, Southwest Medical UniversityDepartment of Clinical, Clinical Medical College, Southwest Medical UniversityDepartment of Clinical, Clinical Medical College, Southwest Medical UniversityDepartment of Clinical, Clinical Medical College, Southwest Medical UniversityDepartment of Clinical, Clinical Medical College, Southwest Medical UniversityDepartment of Oncology, Dazhou Central HospitalResearch Department, Swiss University of Traditional Chinese MedicineGeriatric Department, Dazhou Central HospitalDepartment of Clinical, Clinical Medical College, Southwest Medical UniversityDepartment of Oncology, Dazhou Central HospitalAbstract This study presents a pioneering exploration into the role of aggrephagy-related genes (ARGs) in glioblastoma (GB), a kind of malignant tumor which is highly invasive and resistant to a series of therapy. Utilizing single-cell sequencing to dissect their influence on the tumor microenvironment (TME) and tumorigenesis. By applying non-negative matrix factorization for dimensionality reduction and clustering of single-cell data, distinct cellular subtypes within the TME influenced by ARGs were identified, uncovering their functions and interactions. The investigation extends to validating the prognostic significance of ARGs and their potential in predicting immunotherapy outcomes. Molecular docking analysis of key ARGs further highlights TUBA1C and UBB as promising therapeutic targets, offering novel insights into GB’s complex biology and suggesting a targeted approach for therapy, which is characterized by some crucial pathways in our analysis, including PI3k-akt and TGF-beta pathways. This comprehensive single-cell level examination not only advances our understanding of aggrephagy’s role in GB but also proposes new avenues for prognosis and treatment strategies, emphasizing the critical impact of ARGs on the TME and GB progression.https://doi.org/10.1007/s12672-025-02431-4GBAggrephagyscRNA-seqMolecular dockingDrug targetAggrephagy-related genes |
| spellingShingle | Zipei Chen Shengke Zhang Chenglu Jiang Lai Jiang Haiqing Chen Jinbang Huang Jie Liu Guanhu Yang Xiufang Luo Hao Chi Jiangping Fu Integrating multi-omics data to identify the role of Aggrephagy-related genes in tumor microenvironment and key tumorigenesis factors of GB from the perspective of single-cell sequencing Discover Oncology GB Aggrephagy scRNA-seq Molecular docking Drug target Aggrephagy-related genes |
| title | Integrating multi-omics data to identify the role of Aggrephagy-related genes in tumor microenvironment and key tumorigenesis factors of GB from the perspective of single-cell sequencing |
| title_full | Integrating multi-omics data to identify the role of Aggrephagy-related genes in tumor microenvironment and key tumorigenesis factors of GB from the perspective of single-cell sequencing |
| title_fullStr | Integrating multi-omics data to identify the role of Aggrephagy-related genes in tumor microenvironment and key tumorigenesis factors of GB from the perspective of single-cell sequencing |
| title_full_unstemmed | Integrating multi-omics data to identify the role of Aggrephagy-related genes in tumor microenvironment and key tumorigenesis factors of GB from the perspective of single-cell sequencing |
| title_short | Integrating multi-omics data to identify the role of Aggrephagy-related genes in tumor microenvironment and key tumorigenesis factors of GB from the perspective of single-cell sequencing |
| title_sort | integrating multi omics data to identify the role of aggrephagy related genes in tumor microenvironment and key tumorigenesis factors of gb from the perspective of single cell sequencing |
| topic | GB Aggrephagy scRNA-seq Molecular docking Drug target Aggrephagy-related genes |
| url | https://doi.org/10.1007/s12672-025-02431-4 |
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