Differential responses of root and leaf-associated microbiota to continuous monocultures
Abstract Continuous monocultures alter the composition and function of root-associated microbiota, and thus compromise crop health and productivity. In comparison, little is known about how leaf-associated microbiota respond to continuous monocultures. Here, we profiled root and leaf-associated micr...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Environmental Microbiome |
| Online Access: | https://doi.org/10.1186/s40793-025-00675-9 |
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| author | Hao-Ran Li Xiang-Yu Zhang Kai-Ling He Xin Xu Xin-Wen Chen Yaseen Ullah Ting-Ting Zhang Yan Chen Chuan-Chao Dai Wei Zhang |
| author_facet | Hao-Ran Li Xiang-Yu Zhang Kai-Ling He Xin Xu Xin-Wen Chen Yaseen Ullah Ting-Ting Zhang Yan Chen Chuan-Chao Dai Wei Zhang |
| author_sort | Hao-Ran Li |
| collection | DOAJ |
| description | Abstract Continuous monocultures alter the composition and function of root-associated microbiota, and thus compromise crop health and productivity. In comparison, little is known about how leaf-associated microbiota respond to continuous monocultures. Here, we profiled root and leaf-associated microbiota of peanut plants under monocropping and rotation conditions. Additionally, their protective effects against root pathogen Fusarium oxysporum and leaf pathogen Alternaria alstroemeriae were evaluated. We found that monocropping increased root and leaf disease severity. Meanwhile, the peanut growth and productivity were inhibited by monocropping. Microbiota analysis revealed that monocropping reduced rhizosphere microbial population and diversity, while increased leaf epiphytic microbial population and did not influence leaf epiphytic microbial diversity. Cropping conditions had a greater impact on the microbiota composition of leaf epiphytes than that of the rhizosphere. Moreover, in vitro and in vivo experiments, combined with correlation analyses showed that monocropping weakened the antagonistic activity of rhizosphere microbiota against F. oxysporum and root rot disease. This effect may be associated with the depletion of Bacillus sp. and Sphingomonas sp.. By contrast, leaf epiphytic microbiota under monocropping exhibited greater inhibition of A. alstroemeriae growth and leaf spot control. Together, our results demonstrated a differential response pattern of root and leaf-associated microbiota to continuous monocultures. |
| format | Article |
| id | doaj-art-b60a9a34bbdc487887f1daeb72e3934a |
| institution | Kabale University |
| issn | 2524-6372 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Environmental Microbiome |
| spelling | doaj-art-b60a9a34bbdc487887f1daeb72e3934a2025-02-02T12:43:16ZengBMCEnvironmental Microbiome2524-63722025-01-0120111510.1186/s40793-025-00675-9Differential responses of root and leaf-associated microbiota to continuous monoculturesHao-Ran Li0Xiang-Yu Zhang1Kai-Ling He2Xin Xu3Xin-Wen Chen4Yaseen Ullah5Ting-Ting Zhang6Yan Chen7Chuan-Chao Dai8Wei Zhang9Jiangsu Key Laboratory for Pathogens and Ecosystems, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal UniversityJiangsu Key Laboratory for Pathogens and Ecosystems, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal UniversityJiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal UniversityJiangsu Key Laboratory for Pathogens and Ecosystems, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal UniversityJiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal UniversityJiangsu Key Laboratory for Pathogens and Ecosystems, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal UniversityJiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal UniversityState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of ScienceJiangsu Key Laboratory for Pathogens and Ecosystems, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal UniversityJiangsu Key Laboratory for Pathogens and Ecosystems, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal UniversityAbstract Continuous monocultures alter the composition and function of root-associated microbiota, and thus compromise crop health and productivity. In comparison, little is known about how leaf-associated microbiota respond to continuous monocultures. Here, we profiled root and leaf-associated microbiota of peanut plants under monocropping and rotation conditions. Additionally, their protective effects against root pathogen Fusarium oxysporum and leaf pathogen Alternaria alstroemeriae were evaluated. We found that monocropping increased root and leaf disease severity. Meanwhile, the peanut growth and productivity were inhibited by monocropping. Microbiota analysis revealed that monocropping reduced rhizosphere microbial population and diversity, while increased leaf epiphytic microbial population and did not influence leaf epiphytic microbial diversity. Cropping conditions had a greater impact on the microbiota composition of leaf epiphytes than that of the rhizosphere. Moreover, in vitro and in vivo experiments, combined with correlation analyses showed that monocropping weakened the antagonistic activity of rhizosphere microbiota against F. oxysporum and root rot disease. This effect may be associated with the depletion of Bacillus sp. and Sphingomonas sp.. By contrast, leaf epiphytic microbiota under monocropping exhibited greater inhibition of A. alstroemeriae growth and leaf spot control. Together, our results demonstrated a differential response pattern of root and leaf-associated microbiota to continuous monocultures.https://doi.org/10.1186/s40793-025-00675-9 |
| spellingShingle | Hao-Ran Li Xiang-Yu Zhang Kai-Ling He Xin Xu Xin-Wen Chen Yaseen Ullah Ting-Ting Zhang Yan Chen Chuan-Chao Dai Wei Zhang Differential responses of root and leaf-associated microbiota to continuous monocultures Environmental Microbiome |
| title | Differential responses of root and leaf-associated microbiota to continuous monocultures |
| title_full | Differential responses of root and leaf-associated microbiota to continuous monocultures |
| title_fullStr | Differential responses of root and leaf-associated microbiota to continuous monocultures |
| title_full_unstemmed | Differential responses of root and leaf-associated microbiota to continuous monocultures |
| title_short | Differential responses of root and leaf-associated microbiota to continuous monocultures |
| title_sort | differential responses of root and leaf associated microbiota to continuous monocultures |
| url | https://doi.org/10.1186/s40793-025-00675-9 |
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