Separation, Purification, and Activity Analysis of Angiotensin Converting Enzyme Inhibitory Peptides in Enzymatically Hydrolyzed Egg Yolk of Chinese Soft-Shelled Turtle
Angiotensin converting enzyme (ACE) inhibitory peptides were separated and purified from an enzymatic hydrolysate of egg yolk from Chinese soft-shelled turtle (Pelodiscus sinensis) by sequential ultrafiltration and gel filtration chromatography. Liquid chromatography-tandem mass spectrometry (LC-MS/...
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China Food Publishing Company
2025-01-01
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-1-005.pdf |
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| author | LIU Huayu, LIAO Pengying, ZHANG Tianfeng, ZHANG Xinrui, DENG Yunning, LI Yaohua, WEI Jinrui, CHEN Jun |
| author_facet | LIU Huayu, LIAO Pengying, ZHANG Tianfeng, ZHANG Xinrui, DENG Yunning, LI Yaohua, WEI Jinrui, CHEN Jun |
| author_sort | LIU Huayu, LIAO Pengying, ZHANG Tianfeng, ZHANG Xinrui, DENG Yunning, LI Yaohua, WEI Jinrui, CHEN Jun |
| collection | DOAJ |
| description | Angiotensin converting enzyme (ACE) inhibitory peptides were separated and purified from an enzymatic hydrolysate of egg yolk from Chinese soft-shelled turtle (Pelodiscus sinensis) by sequential ultrafiltration and gel filtration chromatography. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was utilized for the identification of the active peptides, and bioinformatics tools were utilized for activity evaluation. The peptides with strong ACE inhibitory activity were synthesized and verified, and molecular docking was used to analyze the interaction between the active peptides and ACE. The results showed that the hydrolysis degree of soft-shelled turtle egg yolk by bromelain was (17.70 ± 0.34)%, and the half maximal inhibitory concentration (IC50) of ACE inhibitory activity was (0.210 ± 0.019) mg/mL. Altogether, 36 peptides were identified from fraction F3 with ACE inhibitory activity. Six peptides with higher activity scores were selected for synthesis and activity verification, among which peptides YNGIWPRD and ASDILPKK exhibited strong ACE inhibitory activities with IC50 of (0.019 00 ± 0.000 36) and (0.170 0 ± 0.001 3) mg/mL, respectively. The molecular docking results showed that both peptides bounded to ACE tightly through multiple hydrogen bonds. In conclusion, two new ACE inhibitory peptides were selected from the egg yolk of Chinese soft-shelled turtle. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-b5ebaba22eee42b59790dc504a741c552025-02-05T09:08:12ZengChina Food Publishing CompanyShipin Kexue1002-66302025-01-01461404810.7506/spkx1002-6630-20231219-160Separation, Purification, and Activity Analysis of Angiotensin Converting Enzyme Inhibitory Peptides in Enzymatically Hydrolyzed Egg Yolk of Chinese Soft-Shelled TurtleLIU Huayu, LIAO Pengying, ZHANG Tianfeng, ZHANG Xinrui, DENG Yunning, LI Yaohua, WEI Jinrui, CHEN Jun0(1. College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China; 2. Key Laboratory of TCM Extraction and Purification and Quality Analysis, National Demonstration Center for Experimental Traditional Chinese Pharmacology, Guangxi University of Chinese Medicine, Nanning 530200, China; 3. Teaching Experiment and Training Centre, Guangxi University of Chinese Medicine, Nanning 530200, China; 4. Guangxi Scientific Research Centre of Traditional Chinese Medicine, Guangxi University of Chinese Medicine, Nanning 530200, China)Angiotensin converting enzyme (ACE) inhibitory peptides were separated and purified from an enzymatic hydrolysate of egg yolk from Chinese soft-shelled turtle (Pelodiscus sinensis) by sequential ultrafiltration and gel filtration chromatography. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was utilized for the identification of the active peptides, and bioinformatics tools were utilized for activity evaluation. The peptides with strong ACE inhibitory activity were synthesized and verified, and molecular docking was used to analyze the interaction between the active peptides and ACE. The results showed that the hydrolysis degree of soft-shelled turtle egg yolk by bromelain was (17.70 ± 0.34)%, and the half maximal inhibitory concentration (IC50) of ACE inhibitory activity was (0.210 ± 0.019) mg/mL. Altogether, 36 peptides were identified from fraction F3 with ACE inhibitory activity. Six peptides with higher activity scores were selected for synthesis and activity verification, among which peptides YNGIWPRD and ASDILPKK exhibited strong ACE inhibitory activities with IC50 of (0.019 00 ± 0.000 36) and (0.170 0 ± 0.001 3) mg/mL, respectively. The molecular docking results showed that both peptides bounded to ACE tightly through multiple hydrogen bonds. In conclusion, two new ACE inhibitory peptides were selected from the egg yolk of Chinese soft-shelled turtle.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-1-005.pdfchinese soft-shelled turtle egg yolk; angiotensin converting enzyme inhibitory peptide; isolation and purification; bioinformatics; molecular docking |
| spellingShingle | LIU Huayu, LIAO Pengying, ZHANG Tianfeng, ZHANG Xinrui, DENG Yunning, LI Yaohua, WEI Jinrui, CHEN Jun Separation, Purification, and Activity Analysis of Angiotensin Converting Enzyme Inhibitory Peptides in Enzymatically Hydrolyzed Egg Yolk of Chinese Soft-Shelled Turtle Shipin Kexue chinese soft-shelled turtle egg yolk; angiotensin converting enzyme inhibitory peptide; isolation and purification; bioinformatics; molecular docking |
| title | Separation, Purification, and Activity Analysis of Angiotensin Converting Enzyme Inhibitory Peptides in Enzymatically Hydrolyzed Egg Yolk of Chinese Soft-Shelled Turtle |
| title_full | Separation, Purification, and Activity Analysis of Angiotensin Converting Enzyme Inhibitory Peptides in Enzymatically Hydrolyzed Egg Yolk of Chinese Soft-Shelled Turtle |
| title_fullStr | Separation, Purification, and Activity Analysis of Angiotensin Converting Enzyme Inhibitory Peptides in Enzymatically Hydrolyzed Egg Yolk of Chinese Soft-Shelled Turtle |
| title_full_unstemmed | Separation, Purification, and Activity Analysis of Angiotensin Converting Enzyme Inhibitory Peptides in Enzymatically Hydrolyzed Egg Yolk of Chinese Soft-Shelled Turtle |
| title_short | Separation, Purification, and Activity Analysis of Angiotensin Converting Enzyme Inhibitory Peptides in Enzymatically Hydrolyzed Egg Yolk of Chinese Soft-Shelled Turtle |
| title_sort | separation purification and activity analysis of angiotensin converting enzyme inhibitory peptides in enzymatically hydrolyzed egg yolk of chinese soft shelled turtle |
| topic | chinese soft-shelled turtle egg yolk; angiotensin converting enzyme inhibitory peptide; isolation and purification; bioinformatics; molecular docking |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-1-005.pdf |
| work_keys_str_mv | AT liuhuayuliaopengyingzhangtianfengzhangxinruidengyunningliyaohuaweijinruichenjun separationpurificationandactivityanalysisofangiotensinconvertingenzymeinhibitorypeptidesinenzymaticallyhydrolyzedeggyolkofchinesesoftshelledturtle |