Assessing spacer acquisition rates in E. coli type I-E CRISPR arrays

Assessing spacer acquisition rates in E. coli type I-E CRISPR arrays

CRISPR/Cas is an adaptive defense mechanism protecting prokaryotes from viruses and other potentially harmful genetic elements. Through an adaptation process, short “spacer” sequences, captured from these elements and incorporated into a CRISPR array, provide target specificity for the immune respon...

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Bibliographic Details
Main Authors: Luke J. Peach, Haoyun Zhang, Brian P. Weaver, James Q. Boedicker
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-01-01
Series:Frontiers in Microbiology
Subjects:
CRISPR adaptation
type I-E CRISPR
Cas1–Cas2
array expansion
spacer acquisition rates
non-homologous end joining
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2024.1498959/full
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