cfDNA Key genomic markers in HCV-Induced hepatocellular carcinoma in Egyptian patients

cfDNA Key genomic markers in HCV-Induced hepatocellular carcinoma in Egyptian patients

Background: Malignant liver disease is among the highest in the world, with hepatocellular carcinoma (HCC) accounting for up to 90 % of all cases. In Egypt, HCC poses a significant public-health concern, representing 47.17 % of cancer cases. The high incidence of hepatitis C virus (HCV) in the Egypt...

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Bibliographic Details
Main Authors: Mohamed Khalifa, Ahmed A. Hmed, Khaled S. Elfeky, Sayed Bakry, Manal El Hamshary, Ahmed R. Sofy
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Journal of Genetic Engineering and Biotechnology
Subjects:
cfDNA
HCV
Tumor signature
HCC
NGS
Somatic mutation
Online Access:http://www.sciencedirect.com/science/article/pii/S1687157X25000770
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Summary:Background: Malignant liver disease is among the highest in the world, with hepatocellular carcinoma (HCC) accounting for up to 90 % of all cases. In Egypt, HCC poses a significant public-health concern, representing 47.17 % of cancer cases. The high incidence of hepatitis C virus (HCV) in the Egypt was a major predisposing factor for HCC. Material: This study included 63 Egyptian HCC patients, 55 % of whom had a history of HCV infection. Methods: Using a paired sampling strategy, approximately 2800 COSMIC mutations from 50 oncogenes and tumor-suppressor genes were NGS sequenced. Results: Total of 381 somatic mutations were identified, 91 mutations detected in the HCC group and 291 in the HCV-related HCC group. The top 10 mutated genes in the non-HCV group were TP53, ATM, EGFR, CDH1, FGFR1, MET, SMAD4, ERBB2, FLT3, and FBXW7, while in the HCV-related HCC group, genes were KIT, ATM, TP53, APC, FBXW7, KDR, RB1, SMAD4, EGFR, and PIK3CA. Conclusion: The present study represents the first comprehensive somatic mutation profile in HCC Egyptian patients. This finding suggests that HCV viral infection played a direct and indirect role in increasing the somatic mutation burden in HCV-related HCC patients and opens new promises of targeted therapies for those patients.
ISSN:1687-157X

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