Prevalence and phylogenetic analysis of Mycobacterium avium subsp. paratuberculosis in cattle in Mosul city, Iraq
Mycobacterium avium subsp. paratuberculosis (MAP) is a globally distributed infection that causes chronic enteritis, fluctuating milk production, emaciation, and various degrees of diarrhea. It is also a public health concern. This task investigates the molecular prevalence of MAP in cows along with...
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University of Mosul, College of Veterinary Medicine
2025-01-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_186175_8fe9e3620f89e552e04bdd9d7e452000.pdf |
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| author | Sadam D. Hasan Mohammad A. Altaliby Amer H. Taha Karam M. Abdulrazzaq |
| author_facet | Sadam D. Hasan Mohammad A. Altaliby Amer H. Taha Karam M. Abdulrazzaq |
| author_sort | Sadam D. Hasan |
| collection | DOAJ |
| description | Mycobacterium avium subsp. paratuberculosis (MAP) is a globally distributed infection that causes chronic enteritis, fluctuating milk production, emaciation, and various degrees of diarrhea. It is also a public health concern. This task investigates the molecular prevalence of MAP in cows along with a phylogenetic analysis of this microorganism in Mosul City, Iraq. 176 fecal specimens were taken from healthy, diarrheic and emaciated cows of different ages and origins. The detection of gene IS900 of MAP revealed that 5.6% (10/176) of tested cows were confirmed using molecular technique. No significant prevalence between the cow’s origin and a significantly higher prevalence of the MAP was recorded on cows over 5 years old and with symptoms. Furthermore, in this study, two sequences of Mycobacterium avium subsp. paratuberculosis were deposited in GenBank (PP976335.1 and PP976335.1); phylogenetically the study strains showed 100% identity near MAP strain in Saudi Arabia under the accession number (MN928512.1) and 99.85% identity with Spain, Germany and South Korea strain accession numbers (FJ775181.1, CP053068.1 and CP033909.1) respectively. Overall, this result showed that the IS900 gene may be used as a molecular tool to identify MAP in cows. In order to monitor, treat, and manage paratuberculosis in cows in Mosul, Iraq, further epidemiological research and isolation are needed. |
| format | Article |
| id | doaj-art-b5bcf7df662e4d5b910b59e9b44212b8 |
| institution | Kabale University |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| publishDate | 2025-01-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj-art-b5bcf7df662e4d5b910b59e9b44212b82025-02-04T13:11:43ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552025-01-0139115516210.33899/ijvs.2024.154672.3991186175Prevalence and phylogenetic analysis of Mycobacterium avium subsp. paratuberculosis in cattle in Mosul city, IraqSadam D. Hasan0Mohammad A. Altaliby1Amer H. Taha2Karam M. Abdulrazzaq3Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Internal and Preventive Medicine, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Internal and Preventive Medicine, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Internal and Preventive Medicine, College of Veterinary Medicine, University of Mosul, Mosul, IraqMycobacterium avium subsp. paratuberculosis (MAP) is a globally distributed infection that causes chronic enteritis, fluctuating milk production, emaciation, and various degrees of diarrhea. It is also a public health concern. This task investigates the molecular prevalence of MAP in cows along with a phylogenetic analysis of this microorganism in Mosul City, Iraq. 176 fecal specimens were taken from healthy, diarrheic and emaciated cows of different ages and origins. The detection of gene IS900 of MAP revealed that 5.6% (10/176) of tested cows were confirmed using molecular technique. No significant prevalence between the cow’s origin and a significantly higher prevalence of the MAP was recorded on cows over 5 years old and with symptoms. Furthermore, in this study, two sequences of Mycobacterium avium subsp. paratuberculosis were deposited in GenBank (PP976335.1 and PP976335.1); phylogenetically the study strains showed 100% identity near MAP strain in Saudi Arabia under the accession number (MN928512.1) and 99.85% identity with Spain, Germany and South Korea strain accession numbers (FJ775181.1, CP053068.1 and CP033909.1) respectively. Overall, this result showed that the IS900 gene may be used as a molecular tool to identify MAP in cows. In order to monitor, treat, and manage paratuberculosis in cows in Mosul, Iraq, further epidemiological research and isolation are needed.https://www.vetmedmosul.com/article_186175_8fe9e3620f89e552e04bdd9d7e452000.pdfprevalencemycobacteriumphylogeneticis900 genepcr technique |
| spellingShingle | Sadam D. Hasan Mohammad A. Altaliby Amer H. Taha Karam M. Abdulrazzaq Prevalence and phylogenetic analysis of Mycobacterium avium subsp. paratuberculosis in cattle in Mosul city, Iraq Iraqi Journal of Veterinary Sciences prevalence mycobacterium phylogenetic is900 gene pcr technique |
| title | Prevalence and phylogenetic analysis of Mycobacterium avium subsp. paratuberculosis in cattle in Mosul city, Iraq |
| title_full | Prevalence and phylogenetic analysis of Mycobacterium avium subsp. paratuberculosis in cattle in Mosul city, Iraq |
| title_fullStr | Prevalence and phylogenetic analysis of Mycobacterium avium subsp. paratuberculosis in cattle in Mosul city, Iraq |
| title_full_unstemmed | Prevalence and phylogenetic analysis of Mycobacterium avium subsp. paratuberculosis in cattle in Mosul city, Iraq |
| title_short | Prevalence and phylogenetic analysis of Mycobacterium avium subsp. paratuberculosis in cattle in Mosul city, Iraq |
| title_sort | prevalence and phylogenetic analysis of mycobacterium avium subsp paratuberculosis in cattle in mosul city iraq |
| topic | prevalence mycobacterium phylogenetic is900 gene pcr technique |
| url | https://www.vetmedmosul.com/article_186175_8fe9e3620f89e552e04bdd9d7e452000.pdf |
| work_keys_str_mv | AT sadamdhasan prevalenceandphylogeneticanalysisofmycobacteriumaviumsubspparatuberculosisincattleinmosulcityiraq AT mohammadaaltaliby prevalenceandphylogeneticanalysisofmycobacteriumaviumsubspparatuberculosisincattleinmosulcityiraq AT amerhtaha prevalenceandphylogeneticanalysisofmycobacteriumaviumsubspparatuberculosisincattleinmosulcityiraq AT karammabdulrazzaq prevalenceandphylogeneticanalysisofmycobacteriumaviumsubspparatuberculosisincattleinmosulcityiraq |