CYP1A1 Relieves Lipopolysaccharide-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells
The expression of cytochrome P4501A1 (CYP1A1) enzyme is changed in various organs during the host response to inflammation or infection, leading to alterations in the metabolism of endogenous and exogenous compounds. Results of this study showed that CYP1A1 expression was significantly downregulated...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/4093285 |
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| _version_ | 1832551421012082688 |
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| author | Wen-Yao Zhang Hao Wang Shaopei Qi Xixi Wang Xueru Li Kun Zhou Yong Zhang Ming-Qing Gao |
| author_facet | Wen-Yao Zhang Hao Wang Shaopei Qi Xixi Wang Xueru Li Kun Zhou Yong Zhang Ming-Qing Gao |
| author_sort | Wen-Yao Zhang |
| collection | DOAJ |
| description | The expression of cytochrome P4501A1 (CYP1A1) enzyme is changed in various organs during the host response to inflammation or infection, leading to alterations in the metabolism of endogenous and exogenous compounds. Results of this study showed that CYP1A1 expression was significantly downregulated in the mammary tissue of bovine with mastitis, in inflammatory epithelial cells (INEs) extracted from the tissue, and in lipopolysaccharide- (LPS-) induced INEs compared with their corresponding counterparts. Overexpression of CYP1A1 in bovine mammary epithelial cells alleviated the LPS-induced inhibition of epithelial proliferation, abated the LPS-induced increase of gene expression and protein secretion of inflammatory cytokine tumor necrosis factor-α and interleukin-6, and attenuated the LPS-induced activation of NF-κB signaling. These findings suggest that CYP1A1 has immense potential in the regulation of inflammatory responses in bovine mammary epithelial cells during mastitis and may serve as a useful therapeutic target in mitigating injuries caused by inflammatory overreaction. |
| format | Article |
| id | doaj-art-b594caee9bce4694888bc8031b326eea |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-b594caee9bce4694888bc8031b326eea2025-02-03T06:01:31ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/40932854093285CYP1A1 Relieves Lipopolysaccharide-Induced Inflammatory Responses in Bovine Mammary Epithelial CellsWen-Yao Zhang0Hao Wang1Shaopei Qi2Xixi Wang3Xueru Li4Kun Zhou5Yong Zhang6Ming-Qing Gao7College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, ChinaInnovation Experimental College, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, ChinaThe expression of cytochrome P4501A1 (CYP1A1) enzyme is changed in various organs during the host response to inflammation or infection, leading to alterations in the metabolism of endogenous and exogenous compounds. Results of this study showed that CYP1A1 expression was significantly downregulated in the mammary tissue of bovine with mastitis, in inflammatory epithelial cells (INEs) extracted from the tissue, and in lipopolysaccharide- (LPS-) induced INEs compared with their corresponding counterparts. Overexpression of CYP1A1 in bovine mammary epithelial cells alleviated the LPS-induced inhibition of epithelial proliferation, abated the LPS-induced increase of gene expression and protein secretion of inflammatory cytokine tumor necrosis factor-α and interleukin-6, and attenuated the LPS-induced activation of NF-κB signaling. These findings suggest that CYP1A1 has immense potential in the regulation of inflammatory responses in bovine mammary epithelial cells during mastitis and may serve as a useful therapeutic target in mitigating injuries caused by inflammatory overreaction.http://dx.doi.org/10.1155/2018/4093285 |
| spellingShingle | Wen-Yao Zhang Hao Wang Shaopei Qi Xixi Wang Xueru Li Kun Zhou Yong Zhang Ming-Qing Gao CYP1A1 Relieves Lipopolysaccharide-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells Mediators of Inflammation |
| title | CYP1A1 Relieves Lipopolysaccharide-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells |
| title_full | CYP1A1 Relieves Lipopolysaccharide-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells |
| title_fullStr | CYP1A1 Relieves Lipopolysaccharide-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells |
| title_full_unstemmed | CYP1A1 Relieves Lipopolysaccharide-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells |
| title_short | CYP1A1 Relieves Lipopolysaccharide-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells |
| title_sort | cyp1a1 relieves lipopolysaccharide induced inflammatory responses in bovine mammary epithelial cells |
| url | http://dx.doi.org/10.1155/2018/4093285 |
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