Comparative Analysis of Malaria Diagnosis Using Microscopy and Rapid Diagnostic Test (RDT) in Ijebu-Igbo North Local Government, Southwest Nigeria

Malaria remains one of the greatest concerns for the African population. To curb malaria, certain strategies need to be adopted like a quick diagnosis of the parasite within the human body, maintenance of proper environmental hygiene and use of other control methods. Diagnosis of malaria is often a...

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Main Authors: Sherifat Tolulope Akindele, Jumoke Bukunola Bilesanmi–Awoderu, Opeyemi Olumuyiwa Otuewu, Adetutu Mariam Adetunji
Format: Article
Language:English
Published: Umaru Musa Yar'adua University, Katsina, Nigeria 2021-12-01
Series:UMYU Journal of Microbiology Research
Subjects:
Online Access:https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/71
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author Sherifat Tolulope Akindele
Jumoke Bukunola Bilesanmi–Awoderu
Opeyemi Olumuyiwa Otuewu
Adetutu Mariam Adetunji
author_facet Sherifat Tolulope Akindele
Jumoke Bukunola Bilesanmi–Awoderu
Opeyemi Olumuyiwa Otuewu
Adetutu Mariam Adetunji
author_sort Sherifat Tolulope Akindele
collection DOAJ
description Malaria remains one of the greatest concerns for the African population. To curb malaria, certain strategies need to be adopted like a quick diagnosis of the parasite within the human body, maintenance of proper environmental hygiene and use of other control methods. Diagnosis of malaria is often achieved using Microscopy, Rapid Diagnostic Test and Molecular Technique. This study aims to compare the effectiveness of Microscopy and Rapid Diagnostic Testing in diagnosing malaria infection in patients at the General Hospital, Ijebu-Igbo, Ogun State, Nigeria which also falls under a malaria-endemic region using 150 study participants. Blood samples were collected from study participants having malaria symptoms using ethylenediamine tetra-acetic acid (EDTA) container. The screening was done using microscopy method and Rapid Diagnostic Test. The data generated were analyzed using the Statistical Package for the Social Sciences (SPSS) version 19. The statistical parameter that was used for the analysis of the data was Pearson's Chi-Square Test, P at 0.05. The result shows the prevalence of malaria obtained through microscopic examination was 120 (80%) considerably more than RDT 54 (36%). These findings confirmed that microscopy is the gold standard in malaria diagnosis due to its high sensitivity, which allows it to detect parasites even at low counts. However, RDT has a specificity of 93.3% and a sensitivity of 92.2%, indicating that it is also effective when the parasite load is high. However, when compared to RDT kits for malaria diagnosis, microscopic analysis showed a higher sensitivity (100%); nevertheless, RDT may be a useful tool for rapid intervention to avoid the dangers associated with delayed diagnosis.
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issn 2616-0668
2814-1822
language English
publishDate 2021-12-01
publisher Umaru Musa Yar'adua University, Katsina, Nigeria
record_format Article
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spelling doaj-art-b585577fba014a40970ac8486c6a913e2025-08-20T02:28:00ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222021-12-016210.47430/ujmr.2162.00871Comparative Analysis of Malaria Diagnosis Using Microscopy and Rapid Diagnostic Test (RDT) in Ijebu-Igbo North Local Government, Southwest NigeriaSherifat Tolulope Akindele0Jumoke Bukunola Bilesanmi–Awoderu1Opeyemi Olumuyiwa Otuewu2Adetutu Mariam Adetunji3Department of Science Laboratory Technology, Abraham Adesanya Polytechnic, Ijebu-Igbo, Ogun State NigeriaDepartment of Science Laboratory Technology, Abraham Adesanya Polytechnic, Ijebu-Igbo, Ogun State NigeriaDepartment of Science Laboratory Technology, Abraham Adesanya Polytechnic, Ijebu-Igbo, Ogun State NigeriaDepartment of Science Laboratory Technology, Abraham Adesanya Polytechnic, Ijebu-Igbo, Ogun State Nigeria Malaria remains one of the greatest concerns for the African population. To curb malaria, certain strategies need to be adopted like a quick diagnosis of the parasite within the human body, maintenance of proper environmental hygiene and use of other control methods. Diagnosis of malaria is often achieved using Microscopy, Rapid Diagnostic Test and Molecular Technique. This study aims to compare the effectiveness of Microscopy and Rapid Diagnostic Testing in diagnosing malaria infection in patients at the General Hospital, Ijebu-Igbo, Ogun State, Nigeria which also falls under a malaria-endemic region using 150 study participants. Blood samples were collected from study participants having malaria symptoms using ethylenediamine tetra-acetic acid (EDTA) container. The screening was done using microscopy method and Rapid Diagnostic Test. The data generated were analyzed using the Statistical Package for the Social Sciences (SPSS) version 19. The statistical parameter that was used for the analysis of the data was Pearson's Chi-Square Test, P at 0.05. The result shows the prevalence of malaria obtained through microscopic examination was 120 (80%) considerably more than RDT 54 (36%). These findings confirmed that microscopy is the gold standard in malaria diagnosis due to its high sensitivity, which allows it to detect parasites even at low counts. However, RDT has a specificity of 93.3% and a sensitivity of 92.2%, indicating that it is also effective when the parasite load is high. However, when compared to RDT kits for malaria diagnosis, microscopic analysis showed a higher sensitivity (100%); nevertheless, RDT may be a useful tool for rapid intervention to avoid the dangers associated with delayed diagnosis. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/71Malaria,Prevalence,Diagnostic,Microscopic,RDT
spellingShingle Sherifat Tolulope Akindele
Jumoke Bukunola Bilesanmi–Awoderu
Opeyemi Olumuyiwa Otuewu
Adetutu Mariam Adetunji
Comparative Analysis of Malaria Diagnosis Using Microscopy and Rapid Diagnostic Test (RDT) in Ijebu-Igbo North Local Government, Southwest Nigeria
UMYU Journal of Microbiology Research
Malaria,
Prevalence,
Diagnostic,
Microscopic,
RDT
title Comparative Analysis of Malaria Diagnosis Using Microscopy and Rapid Diagnostic Test (RDT) in Ijebu-Igbo North Local Government, Southwest Nigeria
title_full Comparative Analysis of Malaria Diagnosis Using Microscopy and Rapid Diagnostic Test (RDT) in Ijebu-Igbo North Local Government, Southwest Nigeria
title_fullStr Comparative Analysis of Malaria Diagnosis Using Microscopy and Rapid Diagnostic Test (RDT) in Ijebu-Igbo North Local Government, Southwest Nigeria
title_full_unstemmed Comparative Analysis of Malaria Diagnosis Using Microscopy and Rapid Diagnostic Test (RDT) in Ijebu-Igbo North Local Government, Southwest Nigeria
title_short Comparative Analysis of Malaria Diagnosis Using Microscopy and Rapid Diagnostic Test (RDT) in Ijebu-Igbo North Local Government, Southwest Nigeria
title_sort comparative analysis of malaria diagnosis using microscopy and rapid diagnostic test rdt in ijebu igbo north local government southwest nigeria
topic Malaria,
Prevalence,
Diagnostic,
Microscopic,
RDT
url https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/71
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